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Product Info Summary
| SKU: | A30897 |
|---|---|
| Size: | 100ul |
| Reactive Species: | Human, Mouse, Rat |
| Host: | Rabbit |
| Application: | ELISA, IF, ICC, WB |
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Product info
Product Name
Anti-Olfactory receptor 5H15 OR5H15 Antibody
SKU/Catalog Number
A30897
Size
100ul
Form
Liquid
Description
Boster Bio Anti-Olfactory receptor 5H15 OR5H15 Antibody (Catalog# A30897). Tested in WB, ICC, IF, ELISA applications. This antibody reacts with Human, Mouse, Rat.
Storage & Handling
Store at -20°C for one year. For short-term storage and frequent use, store at 4°C for up to one month. Avoid repeated freeze-thaw cycles.
Cite This Product
Anti-Olfactory receptor 5H15 OR5H15 Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # A30897)
Host
Rabbit
Contents
PBS containing 50% glycerol, 0.5% stabilizing protein and 0.02% sodium azide.
This antibody is supplied in a stabilized formulation.
Compatibility with conjugation reactions depends on the chemistry of the conjugation method used.
For conjugation methods that are not compatible with the stabilizing components present in this formulation, a carrier-free antibody format is required.
Clonality
Polyclonal
Isotype
Rabbit IgG
Immunogen
A peptide derived from human OR5H15. Immunogen sequence location: 241 - 290
Reactive Species
A30897 is reactive to OR5H15 in Human, Mouse, Rat
Observed Molecular Weight
72 kDa
Calculated molecular weight
35.4 kDa
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
A30897 is guaranteed for ELISA, IF, ICC, WB Boster Guarantee
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
WB 1:500 - 1:2000.
ICC/IF 1:200 - 1:1000.
ELISA: 1:5000.
Not yet tested in other applications.
Validation Images & Assay Conditions
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Western Blot analysis of COLO cells using Olfactory receptor 5H15 Polyclonal Antibody
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Western blot analysis of the lysates from HeLa cells using OR5H15 antibody.
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Immunofluorescence analysis of MCF7 cells, using OR5H15 Antibody. The picture on the right is blocked with the synthesized peptide.
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Western blot analysis of lysates from COLO cells, using OR5H15 Antibody. The lane on the right is blocked with the synthesized peptide.
Specific Publications For Anti-Olfactory receptor 5H15 OR5H15 Antibody (A30897)
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Customer Reviews
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Customer Q&As
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5 Customer Q&As for Anti-Olfactory receptor 5H15 OR5H15 Antibody
Question
Is this A30897 anti-OR5H15 antibody reactive to the isotypes of OR5H15?
Verified Customer
Verified customer
Asked: 2020-03-27
Answer
The immunogen of A30897 anti-OR5H15 antibody is A peptide derived from human OR5H15. Immunogen sequence location: 241 - 290. Could you tell me which isotype you are interested in so I can help see if the immunogen is part of this isotype?
Boster Scientific Support
Answered: 2020-03-27
Question
We are currently using anti-OR5H15 antibody A30897 for human tissue, and we are happy with the IF results. The species of reactivity given in the datasheet says human. Is it true that the antibody can work on pig tissues as well?
Verified Customer
Verified customer
Asked: 2019-11-05
Answer
The anti-OR5H15 antibody (A30897) has not been validated for cross reactivity specifically with pig tissues, though there is a good chance of cross reactivity. We have an innovator award program that if you test this antibody and show it works in pig you can get your next antibody for free. Please contact me if I can help you with anything.
Boster Scientific Support
Answered: 2019-11-05
Question
I was wanting to use your anti-OR5H15 antibody for WB for human testis on frozen tissues, but I want to know if it has been validated for this particular application. Has this antibody been validated and is this antibody a good choice for human testis identification?
Verified Customer
Verified customer
Asked: 2019-10-15
Answer
As indicated on the product datasheet, A30897 anti-OR5H15 antibody has been validated for IF, WB on human tissues. We have an innovator award program that if you test this antibody and show it works in human testis in IHC-frozen, you can get your next antibody for free.
Boster Scientific Support
Answered: 2019-10-15
Question
Will A30897 anti-OR5H15 antibody work on parafin embedded sections? If so, which fixation method do you recommend we use (PFA, paraformaldehyde, other)?
Verified Customer
Verified customer
Asked: 2017-09-05
Answer
As indicated on the product datasheet, A30897 anti-OR5H15 antibody as been tested on WB. It is best to use PFA for fixation because it has better tissue penetration ability. PFA needs to be prepared fresh before use. Long term stored PFA turns into formalin, as the PFA molecules congregate and become formalin.
Boster Scientific Support
Answered: 2017-09-05
Question
I see that the anti-OR5H15 antibody A30897 works with WB, what is the protocol used to produce the result images on the product page?
Verified Customer
Verified customer
Asked: 2017-07-25
Answer
You can find protocols for WB on the "support/technical resources" section of our navigation menu. If you have any further questions, please send an email to support@bosterbio.com
Boster Scientific Support
Answered: 2017-07-25
