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Product Info Summary
| SKU: | A30533 |
|---|---|
| Size: | 100ul |
| Reactive Species: | Human, Mouse, Rat |
| Host: | Rabbit |
| Application: | ELISA, IF, IHC, ICC, WB |
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Product info
Product Name
Anti-ORAV1 ORAOV1 Antibody
SKU/Catalog Number
A30533
Size
100ul
Form
Liquid
Description
Boster Bio Anti-ORAV1 ORAOV1 Antibody (Catalog# A30533). Tested in WB, IHC, ICC, IF, ELISA applications. This antibody reacts with Human, Mouse, Rat.
Storage & Handling
Store at -20°C for one year. For short-term storage and frequent use, store at 4°C for up to one month. Avoid repeated freeze-thaw cycles.
Cite This Product
Anti-ORAV1 ORAOV1 Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # A30533)
Host
Rabbit
Contents
PBS containing 50% glycerol, 0.5% stabilizing protein and 0.02% sodium azide.
This antibody is supplied in a stabilized formulation.
Compatibility with conjugation reactions depends on the chemistry of the conjugation method used.
For conjugation methods that are not compatible with the stabilizing components present in this formulation, a carrier-free antibody format is required.
Clonality
Polyclonal
Isotype
Rabbit IgG
Immunogen
A peptide derived from human ORAV1. Immunogen sequence location: 81-130
Reactive Species
A30533 is reactive to ORAOV1 in Human, Mouse, Rat
Observed Molecular Weight
72 kDa
Calculated molecular weight
15.4 kDa
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
A30533 is guaranteed for ELISA, IF, IHC, ICC, WB Boster Guarantee
Recommend Dilution
WB 1:500 - 1:2000.
IHC 1:100 - 1:300.
ICC/IF 1:200 - 1:1000.
ELISA: 1:10000.
Not yet tested in other applications.
Validation Images & Assay Conditions
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Immunohistochemical analysis of paraffin-embedded human spleen. 1, Antibody was diluted at 1:200(4° overnight). 2, Tris-EDTA,pH9.0 was used for antigen retrieval. 3,Secondary antibody was diluted at 1:200(room temperature, 45min).
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Western Blot analysis of various cells using ORAOV1 Polyclonal Antibody diluted at 1:2000
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Western blot analysis of lysates from 293 and HeLa cells, using ORAV1 Antibody. The lane on the right is blocked with the synthesized peptide.
Specific Publications For Anti-ORAV1 ORAOV1 Antibody (A30533)
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Customer Reviews
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Customer Q&As
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7 Customer Q&As for Anti-ORAV1 ORAOV1 Antibody
Question
We are currently using anti-ORAV1 antibody A30533 for human tissue, and we are well pleased with the WB results. The species of reactivity given in the datasheet says human. Is it true that the antibody can work on dog tissues as well?
Verified Customer
Verified customer
Asked: 2020-02-11
Answer
The anti-ORAV1 antibody (A30533) has not been validated for cross reactivity specifically with dog tissues, though there is a good chance of cross reactivity. We have an innovator award program that if you test this antibody and show it works in dog you can get your next antibody for free. Please contact me if I can help you with anything.
Boster Scientific Support
Answered: 2020-02-11
Question
I have attached the WB image, lot number and protocol we used for vagina using anti-ORAV1 antibody A30533. Please let me know if you require anything else.
Verified Customer
Verified customer
Asked: 2019-12-03
Answer
Thank you very much for the data. Our lab team are working to resolve this as quickly as possible, and we appreciate your patience and understanding! You have provided everything we needed. Please let me know if there is anything you need in the meantime.
Boster Scientific Support
Answered: 2019-12-03
Question
Thanks for helping with my inquiry over the phone. Here are the WB image, lot number and protocol we used for vagina using anti-ORAV1 antibody A30533. Let me know if you need anything else.
Verified Customer
Verified customer
Asked: 2019-07-09
Answer
We appreciate the data. You have provided everything we needed. Our lab team are working to resolve your inquiry as quickly as possible, and we appreciate your patience and understanding! Please let me know if there is anything you need in the meantime.
Boster Scientific Support
Answered: 2019-07-09
Question
Is this A30533 anti-ORAV1 antibody reactive to the isotypes of ORAOV1?
Verified Customer
Verified customer
Asked: 2019-07-03
Answer
The immunogen of A30533 anti-ORAV1 antibody is A peptide derived from human ORAV1. Immunogen sequence location: 81-130. Could you tell me which isotype you are interested in so I can help see if the immunogen is part of this isotype?
Boster Scientific Support
Answered: 2019-07-03
Question
I would like to test anti-ORAV1 antibody A30533 on human vagina for research purposes, then I may be interested in using anti-ORAV1 antibody A30533 for diagnostic purposes as well. Is the antibody suitable for diagnostic purposes?
Verified Customer
Verified customer
Asked: 2019-06-28
Answer
The products we sell, including anti-ORAV1 antibody A30533, are only intended for research use. They would not be suitable for use in diagnostic work. If you have the means to develop a product into diagnostic use, and are interested in collaborating with us and develop our product into an IVD product, please contact us for more discussions.
Boster Scientific Support
Answered: 2019-06-28
Question
My question regarding product A30533, anti-ORAV1 antibody. I was wondering if it would be possible to conjugate this antibody with biotin. I would need it to be without BSA or sodium azide. I am planning on using a buffer exchange of sodium azide with PBS only. Would there be problems for me to conjugate the antibody and store it in -20 degrees in small aliquots?
Verified Customer
Verified customer
Asked: 2018-01-09
Answer
We suggest not storing this antibody with PBS buffer only in -20 degrees. If you want to store it in -20 degrees it is best to add some cryoprotectant like glycerol. If you want carrier free A30533 anti-ORAV1 antibody, we can provide it to you in a special formula with trehalose and/or glycerol. These molecules will not interfere with conjugation chemistry and provide a good level of protection for the antibody from degradation. Please be sure to specify this in your purchase order.
Boster Scientific Support
Answered: 2018-01-09
Question
I was wanting to use your anti-ORAV1 antibody for WB for human vagina on frozen tissues, but I want to know if it has been tested for this particular application. Has this antibody been tested and is this antibody a good choice for human vagina identification?
Verified Customer
Verified customer
Asked: 2017-08-09
Answer
You can see on the product datasheet, A30533 anti-ORAV1 antibody has been validated for WB on human tissues. We have an innovator award program that if you test this antibody and show it works in human vagina in IHC-frozen, you can get your next antibody for free.
Boster Scientific Support
Answered: 2017-08-09
