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Product Info Summary
| SKU: | A30548 |
|---|---|
| Size: | 100ul |
| Reactive Species: | Human, Mouse, Rat |
| Host: | Rabbit |
| Application: | ELISA, IF, ICC, WB |
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Product info
Product Name
Anti-ORCTL-2 Antibody
SKU/Catalog Number
A30548
Size
100ul
Form
Liquid
Description
Boster Bio Anti-ORCTL-2 Antibody (Catalog# A30548). Tested in WB, ICC, IF, ELISA applications. This antibody reacts with Human, Mouse, Rat.
Storage & Handling
Store at -20°C for one year. For short-term storage and frequent use, store at 4°C for up to one month. Avoid repeated freeze-thaw cycles.
Cite This Product
Anti-ORCTL-2 Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # A30548)
Host
Rabbit
Contents
PBS containing 50% glycerol, 0.5% stabilizing protein and 0.02% sodium azide.
This antibody is supplied in a stabilized formulation.
Compatibility with conjugation reactions depends on the chemistry of the conjugation method used.
For conjugation methods that are not compatible with the stabilizing components present in this formulation, a carrier-free antibody format is required.
Clonality
Polyclonal
Isotype
Rabbit IgG
Immunogen
A peptide derived from human ORCTL-2. Immunogen sequence location: 359-408
Reactive Species
A30548 is reactive to SLC22A18 in Human, Mouse, Rat
Observed Molecular Weight
72 kDa
Calculated molecular weight
44.8 kDa
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
A30548 is guaranteed for ELISA, IF, ICC, WB Boster Guarantee
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
WB 1:500 - 1:2000.
ICC/IF 1:200 - 1:1000.
ELISA: 1:10000.
Not yet tested in other applications.
Validation Images & Assay Conditions
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Western Blot analysis of various cells using ORCTL2 Polyclonal Antibody
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Western blot analysis of lysates from COLO205 cells, using ORCTL-2 Antibody. The lane on the right is blocked with the synthesized peptide.
Specific Publications For Anti-ORCTL-2 Antibody (A30548)
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5 Customer Q&As for Anti-ORCTL-2 Antibody
Question
I was wanting to use your anti-ORCTL-2 antibody for WB for human placenta on frozen tissues, but I want to know if it has been validated for this particular application. Has this antibody been validated and is this antibody a good choice for human placenta identification?
Verified Customer
Verified customer
Asked: 2020-05-04
Answer
You can see on the product datasheet, A30548 anti-ORCTL-2 antibody has been validated for WB on human tissues. We have an innovator award program that if you test this antibody and show it works in human placenta in IHC-frozen, you can get your next antibody for free.
Boster Scientific Support
Answered: 2020-05-04
Question
We are currently using anti-ORCTL-2 antibody A30548 for human tissue, and we are happy with the WB results. The species of reactivity given in the datasheet says human. Is it possible that the antibody can work on bovine tissues as well?
Verified Customer
Verified customer
Asked: 2019-10-18
Answer
The anti-ORCTL-2 antibody (A30548) has not been tested for cross reactivity specifically with bovine tissues, but there is a good chance of cross reactivity. We have an innovator award program that if you test this antibody and show it works in bovine you can get your next antibody for free. Please contact me if I can help you with anything.
Boster Scientific Support
Answered: 2019-10-18
Question
Would A30548 anti-ORCTL-2 antibody work on parafin embedded sections? If so, which fixation method do you recommend we use (PFA, paraformaldehyde, other)?
Verified Customer
Verified customer
Asked: 2019-08-09
Answer
As indicated on the product datasheet, A30548 anti-ORCTL-2 antibody as been tested on WB. It is best to use PFA for fixation because it has better tissue penetration ability. PFA needs to be prepared fresh before use. Long term stored PFA turns into formalin, as the PFA molecules congregate and become formalin.
Boster Scientific Support
Answered: 2019-08-09
Question
Would anti-ORCTL-2 antibody A30548 work on canine WB with placenta?
Verified Customer
Verified customer
Asked: 2019-06-18
Answer
Our lab technicians have not validated anti-ORCTL-2 antibody A30548 on canine. You can run a BLAST between canine and the immunogen sequence of anti-ORCTL-2 antibody A30548 to see if they may cross-react. If the sequence homology is close, then you can perform a pilot test. Keep in mind that since we have not validated canine samples, this use of the antibody is not covered by our guarantee. However we have an innovator award program that if you test this antibody and show it works in canine placenta in WB, you can get your next antibody for free.
Boster Scientific Support
Answered: 2019-06-18
Question
My lab would like to test anti-ORCTL-2 antibody A30548 on human placenta for research purposes, then I may be interested in using anti-ORCTL-2 antibody A30548 for diagnostic purposes as well. Is the antibody suitable for diagnostic purposes?
J. Rodriguez
Verified customer
Asked: 2016-02-03
Answer
The products we sell, including anti-ORCTL-2 antibody A30548, are only intended for research use. They would not be suitable for use in diagnostic work. If you have the means to develop a product into diagnostic use, and are interested in collaborating with us and develop our product into an IVD product, please contact us for more discussions.
Boster Scientific Support
Answered: 2016-02-03
