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Product Info Summary
| SKU: | PA1949 |
|---|---|
| Size: | 100 μg/vial |
| Reactive Species: | Human, Mouse, Rat |
| Host: | Rabbit |
| Application: | WB |
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Product info
Product Name
Anti-OSBP1 Antibody
SKU/Catalog Number
PA1949
Size
100 μg/vial
Form
Lyophilized
Description
Boster Bio Anti-OSBP1 Antibody catalog # PA1949. Tested in WB applications. This antibody reacts with Human, Mouse, Rat.
Storage & Handling
Store at -20˚C for one year from date of receipt. After reconstitution, at 4˚C for one month. It can also be aliquotted and stored frozen at -20˚C for six months. Avoid repeated freeze-thaw cycles.
Cite This Product
Anti-OSBP1 Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # PA1949)
Host
Rabbit
Contents
Each vial contains 5mg BSA, 0.9mg NaCl, 0.2mg Na2HPO4, 0.05mg Thimerosal, 0.05mg NaN3.
Clonality
Polyclonal
Isotype
Rabbit IgG
Immunogen
A synthetic peptide corresponding to a sequence in the middle region of human OSBP1, identical to the related rat and mouse sequences.
*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.
Cross-reactivity
No cross-reactivity with other proteins
Reactive Species
PA1949 is reactive to OSBP in Human, Mouse, Rat
Applications
PA1949 is guaranteed for WB Boster Guarantee
Observed Molecular Weight
75 kDa
Calculated molecular weight
89.421kDa
Background of OSBP1
OSBP (Oxysterol-Binding Protein), also called OSBP1, is a protein that in humans is encoded by the OSBP gene. Im et al. (2005) reported the structure of the full-length yeast oxysterol-binding-protein Osh4, a member of the OSBP-related protein (ORP) family, at 1.5- to 1.9-angstrom resolution in complexes with ergosterol, cholesterol, and 7-, 20- and 25-hydroxy cholesterol. Moreira et al. (2001) refined the localization of the OSBP1 gene to chromosome 11q12.1 by radiation hybrid analysis. Wang et al. (2005) found that OSBP functions as a cholesterol-binding scaffolding protein coordinating the activity of 2 phosphatases to control the extracellular signal-regulated kinase (ERK) signaling pathway. Cytosolic OSBP formed an approximately 440-kD oligomer with a member of the PTPPBS family of tyrosine phosphatases, the serine/threonine phosphatase PP2A, and cholesterol.
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Innovating Scientists Reward
If you are the first to review this product, or if you have results for a special sample, species or application this product is not validated in, share your results with us and receive product credits you can use towards any Boster products! Applicable to all scientists worldwide.
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Assay dilution & Images
Reconsitution
Add 0.2ml of distilled water will yield a concentration of 500ug/ml.
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
Western blot, 0.1-0.5μg/ml, Human, Rat, Mouse
Validation Images & Assay Conditions
Click image to see more details
Anti-OSBP1 antibody, PA1949, Western blotting
Lane 1: Rat Kidney Tissue Lysate
Lane 2: Rat Spleen Tissue Lysate
Lane 3: Rat Lung Tissue Lysate
Lane 4: HELA Cell Lysate
Lane 5: A549 Cell Lysate
Protein Target Info & Infographic
Gene/Protein Information For OSBP (Source: Uniprot.org, NCBI)
Gene Name
OSBP
Full Name
Oxysterol-binding protein 1
Weight
89.421kDa
Superfamily
OSBP family
Alternative Names
OSBP1oxysterol-binding protein 1; oxysterol binding protein OSBP OSBP1 oxysterol binding protein oxysterol-binding protein 1
*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".For more info on OSBP, check out the OSBP Infographic
We have 30,000+ of these available, one for each gene! Check them out.
In this infographic, you will see the following information for OSBP: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].
Specific Publications For Anti-OSBP1 Antibody (PA1949)
Hello CJ!
No publications found for PA1949
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Recommended Resources
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Customer Reviews
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Customer Q&As
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3 Customer Q&As for Anti-OSBP1 Antibody
Question
We are currently using anti-OSBP1 antibody PA1949 for rat tissue, and we are happy with the WB results. The species of reactivity given in the datasheet says human, mouse, rat. Is it likely that the antibody can work on zebrafish tissues as well?
Verified Customer
Verified customer
Asked: 2020-01-03
Answer
The anti-OSBP1 antibody (PA1949) has not been validated for cross reactivity specifically with zebrafish tissues, but there is a good chance of cross reactivity. We have an innovator award program that if you test this antibody and show it works in zebrafish you can get your next antibody for free. Please contact me if I can help you with anything.
Boster Scientific Support
Answered: 2020-01-03
Question
I appreciate helping with my inquiry over the phone. Here are the WB image, lot number and protocol we used for leukemic t-cell using anti-OSBP1 antibody PA1949. Let me know if you need anything else.
K. Thomas
Verified customer
Asked: 2014-04-09
Answer
Thank you for the data. You have provided everything we needed. Our lab team are working to resolve your inquiry as quickly as possible, and we appreciate your patience and understanding! Please let me know if there is anything you need in the meantime.
Boster Scientific Support
Answered: 2014-04-09
Question
I was wanting to use to test anti-OSBP1 antibody PA1949 on mouse leukemic t-cell for research purposes, then I may be interested in using anti-OSBP1 antibody PA1949 for diagnostic purposes as well. Is the antibody suitable for diagnostic purposes?
M. Moore
Verified customer
Asked: 2013-01-07
Answer
The products we sell, including anti-OSBP1 antibody PA1949, are only intended for research use. They would not be suitable for use in diagnostic work. If you have the means to develop a product into diagnostic use, and are interested in collaborating with us and develop our product into an IVD product, please contact us for more discussions.
Boster Scientific Support
Answered: 2013-01-07
