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- Table of Contents
TNFRSF11B antibody (A00863) performs well in ELISA, Flow Cytometry, IHC, WB and shows reactivity with Human, Monkey, Mouse, Rat.
- Validated for inter-lot consistency, specificity, and reproducibility
- Committed to quality since 1993
- Over 11 Publications
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Flow Cytometry analysis of U20S cells using anti-TNFRSF11B antibody (A00863).
Overlay histogram showing U20S cells stained with A00863 (Blue line). To facilitate intracellular staining, cells were fixed with 4% paraformaldehyde and permeabilized with permeabilization buffer. The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-TNFRSF11B Antibody (A00863,1μg/1x106 cells) for 30 min at 20°C. DyLight®488 conjugated goat anti-rabbit IgG (BA1127, 5-10μg/1x106 cells) was used as secondary antibody for 30 minutes at 20°C. Isotype control antibody (Green line) was rabbit IgG (1μg/1x106) used under the same conditions. Unlabelled sample without incubation with primary antibody and secondary antibody (Red line) was used as a blank control.
Overlay histogram showing U20S cells stained with A00863 (Blue line). To facilitate intracellular staining, cells were fixed with 4% paraformaldehyde and permeabilized with permeabilization buffer. The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-TNFRSF11B Antibody (A00863,1μg/1x106 cells) for 30 min at 20°C. DyLight®488 conjugated goat anti-rabbit IgG (BA1127, 5-10μg/1x106 cells) was used as secondary antibody for 30 minutes at 20°C. Isotype control antibody (Green line) was rabbit IgG (1μg/1x106) used under the same conditions. Unlabelled sample without incubation with primary antibody and secondary antibody (Red line) was used as a blank control.
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Anti-Osteoprotegerin/TNFRSF11B Antibody Picoband®
TNFRSF11B antibody
| Reactive Species | Host | Application |
|---|---|---|
| Human, Monkey, Mouse, Rat | Rabbit | ELISA, Flow Cytometry, IHC, WB |
Tested Applications
TNFRSF11B antibody was positively detected by WB in:
human HEK293 whole cell, monkey COS-7 whole cell, human Hela whole cell
Suggested blocking solution with 5% non-fat milk or BSA; (*)Recommended protein loading: 20-40 ug per lane
TNFRSF11B antibody was positively detected by IHC in:
human appendicitis tissue, human endometrial carcinoma tissue, human glioma tissue, human glioma tissue, human liver cancer tissue, human liver cancer tissue, human Lung cancer tissue, human Lung cancer tissue, human Lung cancer tissue, human oesophagus squama cancer tissue, rat brain tissue, rat brain tissue, human Cholangiocarcinoma tissue, human mammary cancer tissue, human mammary cancer tissue, human placenta tissue, human rectal cancer tissue, human rectal cancer tissue, human tonsil tissue, human tonsil tissue, mouse brain tissue, human appendicitis tissue
Use TE buffer pH 9.0 for antigen retrieval; (*) citrate buffer pH 6.0 is an alternative.
TNFRSF11B antibody was positively detected by FC in:
U20S cell
Recommend Dilution
Application
Dilution
Species
Western blot
0.25-0.5μg/ml
Human, Monkey, Mouse, Rat
Immunohistochemistry (Paraffin-embedded Section)
0.5-1μg/ml
Human, Mouse, Rat
Flow Cytometry (Fixed)
1-3μg/1x106 cells
Human
ELISA
0.1-0.5μg/ml
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* It is recommended to optimize the amount of this reagent in each experimental system to achieve the best results.
Note: For flow cytometry (FCM), validated for fixed cells only. Not validated for FACS or surface flow cytometry using live cells.
Reactive Data
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WB
IHC
IF
ELISA
Human
Tested
Tested
Recommend
Recommend
Monkey
Tested
Recommend
Rat
Tested
Recommend
Recommend
Mouse
Tested
Recommend
Recommend
Rabbit
Predicted
Predicted
Predicted
Predicted
Dog
Predicted
Predicted
Predicted
Predicted
Bovine
Predicted
Predicted
Predicted
Predicted
Sheep
Predicted
Predicted
Predicted
Predicted
Product Information
Product Name
Anti-Osteoprotegerin/TNFRSF11B Antibody Picoband®
View all TNFRSF11B Antibodies
View all TNFRSF11B Antibodies
Form
Lyophilized
Uniprot
Gene ID
Storage & Handling
Store at -20˚C for one year from date of receipt. After reconstitution, at 4˚C for one month. It can also be aliquotted and stored frozen at -20˚C for six months. Avoid repeated freeze-thaw cycles.
Host
Rabbit
Contents
Each vial contains 4mg Trehalose, 0.9mg NaCl, 0.2mg Na2HPO4, 0.05mg NaN3.
Clonality
Polyclonal
Isotype
Rabbit IgG
Immunogen
E.coli-derived human Osteoprotegerin/TNFRSF11B recombinant protein (Position: Q247-R296).
Cross-Reactivity
No cross-reactivity with other proteins.
Reconstitution
Add 0.2ml of distilled water will yield a concentration of 500ug/ml.
Observed Molecular Weight
55 kDa
Calculated Molecular Weight
46.0 kDa
Background of TNFRSF11B
Tumor necrosis factor receptor superfamily member 11B (TNFRSF11B), also known as OPG, is a protein that in humans is encoded by the TNFRSF11B gene. OPG is a cytokine receptor, and a member of the tumor necrosis factor (TNF) receptor superfamily. By analysis of radiation hybrids, TNFRSF11B gene was mapped to chromosome 8q24. OPG is a decoy receptor for the receptor activator of nuclear factor kappa B ligand (RANKL). By binding RANKL, OPG inhibits nuclear kappa B (NF-κB) which is a central and rapid acting transcription factor for immune-related genes, and a key regulator of inflammation, innate immunity, and cell survival and differentiation. OPG binding to RANKL on osteoblast/stromal cells, blocks the RANKL-RANK ligand interaction between osteoblast/stromal cells and ostepclast precursors.
Cite This Product
Anti-Osteoprotegerin/TNFRSF11B Antibody Picoband® (Boster Biological Technology, Pleasanton CA, USA, Catalog # A00863)
Antibody Homology Across Species
The results generated by this homology analysis tool for commonly used animal species are shown below. For unlisted species, please refer to the homology analysis between the immunogen sequence and the corresponding species.
A homology of ≥90% is recommended. Generally, higher homology indicates a greater potential for cross-species reactivity. However, protein expression levels may vary among different species, tissues, and cell types. Therefore, the homology analysis results are for reference only and do not guarantee antibody cross-reactivity or experimental performance.
Human
100%
High Homology
Monkey
100%
High Homology
Rabbit
94%
High Homology
Dog
94%
High Homology
Rat
90%
High Homology
Cow
90%
High Homology
Sheep
90%
High Homology
Mouse
84%
Moderate Homology
Zebrafish
24%
Low homology
Homology ≥ 90%High Homology
70% ≤ Homology < 90%Moderate Homology
Homology < 70%Low homology
Protocols
Western Blot protocol for TNFRSF11B antibody
Immunohistochemistry (IHC) protocol for TNFRSF11B antibody
Flow Cytometry (FC) protocol for TNFRSF11B antibody
Publications
Application:
Species:
Chemical composition and nutritional properties of Gastrodia elata water extract and its effects in rats with streptozotocin–induced diabetic osteoporosis
Published: 2025-06-03
Journal: Frontiers in Nutrition
Application: IHC
Impact Factor: 5.10
Species: Rat
PMID: 40529421
Adiponectin receptors agonist alters microbiota to improve implant osseointegration in diabetic mice
Published: 2024-08-23
Journal: ORAL DISEASES
Application: IHC
Impact Factor: 2.90
Species: Mouse
PMID: 39177011
Arl13b promotes the proliferation, migration, osteogenesis, and mechanosensation of osteoblasts
Published: 2023-04-11
Journal: TISSUE & CELL
Application: WB
Impact Factor: 2.60
Species: Mouse
PMID: 37058812
Down-regulating of MFN2 promotes vascular calcification via regulating RAS-RAF-ERK1/2 pathway
Published: 2022-06-15
Journal: INTERNATIONAL JOURNAL OF CARDIOLOGY
Application: WB
Impact Factor: 4.04
Species: Mouse
PMID: 35716948
MALAT1 enhanced the proliferation of human osteoblasts treated with ultra‑high molecular weight polyethylene by targeting VEGF via miR‑22‑5p
Published: 2018-01-04
Journal: INTERNATIONAL JOURNAL OF MOLECULAR MEDICINE
Application: WB
Impact Factor: 2.78
Species: Human
PMID: 29328414
Alendronate-loaded hydroxyapatite-TiO2 nanotubes for improved bone formation in osteoporotic rabbits
Published: 2016-01-25
Journal: Journal of Materials Chemistry B
Application: WB
Impact Factor: 4.87
Species: Rat
PMID: 32263109
Anti-osteoporotic activity of puerarin 6″-O-xyloside on ovariectomized mice and its potential mechanism
Published: 2015-04-10
Journal: PHARMACEUTICAL BIOLOGY
Application: WB
Impact Factor: 1.24
Species: Rat
PMID: 25857587
Calcitonin Gene-Related Peptide Inhibits Osteolytic Factors Induced by Osteoblast In Co-Culture System with Breast Cancer
Published: 2014-05-23
Journal: CELL BIOCHEMISTRY AND BIOPHYSICS
Application: WB
Impact Factor: 2.38
Species: Human
PMID: 24853718
Long-term resveratrol treatment prevents ovariectomy-induced osteopenia in rats without hyperplastic effects on the uterus
Published: 2013-09-30
Journal: BRITISH JOURNAL OF NUTRITION
Application: IHC
Impact Factor: 3.30
Species: Rat
PMID: 24073920
Effects of Phytoestrogen α-ZAL and Mechanical Stimulation on Proliferation, Osteoblastic Differentiation, and OPG/RANKL Expression in MC3T3-E1 Pre-Osteoblasts
Published: 2012-08-10
Journal: Cellular and Molecular Bioengineering
Application: WB
Impact Factor: 1.95
Species: Mouse
Porphyromonas endodontalis Lipopolysaccharides Induce RANKL by Mouse Osteoblast in a Way Different from That of Escherichia coli Lipopolysaccharide
Published: 2011-10-02
Journal: JOURNAL OF ENDODONTICS
Application: WB
Impact Factor: 3.29
Species: Mouse
PMID: 22099899
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Product Promise
We promise Anti-Osteoprotegerin/TNFRSF11B Antibody Picoband® performs excellently in relevant applications for ELISA, Flow Cytometry, IHC, WB. If it doesn't perform as promised, we will refund the product. Learn more
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A00863
Buy one primary antibody get one 0.5ml HRP or Biotin secondary antibody for free.
*Sample sizes are prepared on demand and will take extra lead time. (cannot be conjugated)
$370.00
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We promise all of our products perform as described in datasheets.
This product is for Research Use Only. Not for use in diagnostic procedures.
Update date:
Oct 4, 2026