Anti-OTUD5 Antibody

Product Name

Anti-OTUD5 Antibody

View all OTUD5 Antibodies

SKU/Catalog Number

A09744

Size

0.1 mg

Form

Liquid

Description

Boster Bio Anti-OTUD5 Antibody (Catalog # A09744). Tested in ELISA, WB, IHC-P, IF applications. This antibody reacts with Human, Mouse, Rat.

Storage & Handling

OTUD5 antibody can be stored at 4°C for three months and -20°C, stable for up to one year. Avoid repeated freeze-thaw cycles. Antibodies should not be exposed to prolonged high temperatures.

Cite This Product

Anti-OTUD5 Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # A09744)

Host

Rabbit

Contents

OTUD5 Antibody is supplied in PBS containing 0.02% sodium azide.

Clonality

Polyclonal

Isotype

IgG

Immunogen

OTUD5 antibody was raised against a 13 amino acid synthetic peptide near the carboxy terminus of the human OTUD5. The immunogen is located within amino acids 400 - 450 of OTUD5.

Reactive Species

A09744 is reactive to OTUD5 in Human, Mouse, Rat

Observed Molecular Weight

68 kDa

Calculated molecular weight

60.6 kDa

Background of OTUD5

OTUD5 is a member of the OTU (ovarian tumor) domain-containing cysteine protease superfamily. The OTU domain confers deubiquitinase activity and OTUD5 has been shown to suppress the type I interferon (IFN-I)-dependent innate immune response by cleaving the polyubiquitin chain from TRAF3, an essential type I interferon adaptor protein. Cleavage results in disassociation of TRAF3 from a downstream signaling complex containing TBK1 and the disruption of the IFN-I signaling cascade, indicating that OTUD5 acts as a negative regulator of innate immune responses. It has been suggested that by suppressing IFN-I production, OTUD5 may function to inhibit the emergence of certain autoimmune disorders such as systemic lupus erythematosus. Multiple isoforms of OTUD5 are known to exist.

Antibody Validation

Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.

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Applications

A09744 is guaranteed for ELISA, IF, IHC-P, WB Boster Guarantee

Assay Dilutions Recommendation

The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.

OTUD5 antibody can be used for detection of OTUD5 by Western blot at 1 - 2 μg/mL. Antibody can also be used for immunohistochemistry starting at 2.5 μg/mL. For immunofluorescence start at 20 μg/mL.
Antibody validated: Western Blot in human samples; Immunohistochemistry in mouse samples and Immunofluorescence in human samples. All other applications and species not yet tested. Optimal dilutions for each application should be determined by the researcher.

Validation Images & Assay Conditions

See full target information OTUD5

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