Anti-p21 WAF1 CDKN1A Monoclonal Antibody

Product Name

Anti-p21 WAF1 CDKN1A Monoclonal Antibody

View all CDKN1A Antibodies

SKU/Catalog Number

M00145

Size

100ug

Form

Liquid (sterile filtered)

Description

Boster Bio Anti-p21 WAF1 CDKN1A Monoclonal Antibody (Catalog # M00145). Tested in ELISA, IF, WB applications. This antibody reacts with Human.

Storage & Handling

Store vial at -20°C prior to opening. Aliquot contents and freeze at -20°C or below for extended storage. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4°C as an undiluted liquid. Dilute only prior to immediate use. Expiration date is one (1) year from date of opening. (Ship on dry ice.)

Cite This Product

Anti-p21 WAF1 CDKN1A Monoclonal Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # M00145)

Host

Mouse

Contents

0.02 M Potassium Phosphate, 0.5 M Sodium Chloride, pH 7.2, 0.01% (w/v) Sodium Azide

Clonality

Monoclonal

Clone Number

Clone: WA-1 IgG1 kappa

Isotype

IgG1 kappa

Immunogen

This protein A purified monoclonal antibody was produced by repeated immunizations with recombinant human p21 fusion protein.

Reactive Species

M00145 is reactive to CDKN1A in Human

Observed Molecular Weight

42 kDa

Calculated molecular weight

18.1 kDa

Background of CDKN1A

p21WAF1 is a tumor suppressor gene product critical to maintain proper regulatory control of cell division. p21WAF1 inhibits cyclin/cyclin-dependent kinase (cdk) complexes, causing cell cycle arrest by blocking cell division at the S phase. Various mutations of tumor suppressor genes have been associated with different types of cancers. In these cases, somatic gene therapy with wild-type versions of tumor suppressor genes have been contemplated as anti-cancer therapeutic approaches. p21WAF1 contains p53-binding sites in its promoter and expression of p21WAF1 is induced in WI38 fibroblasts with PMA. Anti-p21WAF1 antibody is useful for researches interested in studying tongue and gastrointestinal cancers, as well as, ubiquitin and cyclin binding proteins.

Antibody Validation

Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.

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Applications

M00145 is guaranteed for ELISA, IP, IF, IHC, WB Boster Guarantee

Assay Dilutions Recommendation

The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.

ELISA: 1:5,000 - 1:20,000
IHC: User optimized
IF Microscopy: 1:250
IP: 1:100
WB: 1:2,000 - 1:10,000
Anti-p21/WAF1 has been tested in ELISA, western blot, and immunofluorescence. This product is also suitable for immunohistochemistry. The antibody detects a 21 kDa band corresponding to the p21 protein.  MCF7 cells or UV treated fibroblasts are suggested as a positive control for immunoblotting and immunohistochemistry.   Methanol/acetone fixed tissue is reactive for immunohistochemistry. The antibody also reacts with breast carcinoma on frozen sections (methanol/acetone fixation) and on paraffin sections (formalin fixation and microwave treatment prior to staining).  In ELISA this monoclonal antibody is useful either as a capture antibody (attached to the solid phase) or for detection of p21 protein.  This antibody immunoprecipitates monomeric p21WAF1 and not p21 complexes with other proteins. High level expression of p21WAF1 requires induction of wild-type p53 expression (e.g. via exposure to DNA damaging agents).

Validation Images & Assay Conditions

See full target information CDKN1A

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