Product Info Summary
SKU: | A03781 |
---|---|
Size: | 100ul |
Reactive Species: | Human, Mouse, Rat |
Host: | Rabbit |
Application: | WB |
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Product info
Product Name
Anti-p22-phox CYBA Antibody
View all Cytochrome b245 alpha Antibodies
SKU/Catalog Number
A03781
Size
100ul
Form
Liquid
Description
Boster Bio Anti-p22-phox CYBA Antibody catalog # A03781. Tested in WB applications. This antibody reacts with Human,Mouse,Rat.
Storage & Handling
Store at -20°C for one year. For short term storage and frequent use, store at 4°C for up to one month. Avoid repeated freeze-thaw cycles.
Cite This Product
Anti-p22-phox CYBA Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # A03781)
Host
Rabbit
Contents
Rabbit IgG, 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2
Clonality
Polyclonal
Isotype
IgG
Immunogen
Synthesized peptide derived from human NU6M protein.
*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.
Reactive Species
A03781 is reactive to CYBA in Human, Mouse, Rat
Applications
A03781 is guaranteed for WB Boster Guarantee
Observed Molecular Weight
Calculated molecular weight
21.013kDa
Background of Cytochrome b245 alpha
May catalyze alpha-1,3 and alpha-1,4 glycosidic linkages involved in the expression of Vim-2, Lewis A, Lewis B, sialyl Lewis X and Lewis X/SSEA-1 antigens. May be involved in blood group Lewis determination; Lewis-positive (Le+) individuals have an active enzyme while Lewis-negative (Le-) individuals have an inactive enzyme. Also acts on the corresponding 1,4-galactosyl derivative, forming 1,3-L-fucosyl links.
Kukowska-Latallo J.F., Genes Dev. 4:1288-1303(1990).
Cameron H.S., J. Biol. Chem. 270:20112-20122(1995).
Rahim I., Submitted (FEB-1999) to the EMBL/GenBank/DDBJ databases.
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
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Assay dilution & Images
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
WB: 1:500-1:1000
Validation Images & Assay Conditions
Click image to see more details
Figure 1. Western blotting validation for Anti-p22-phox CYBA Antibody A03781
Western blot (WB) analysis of p22-phox polyclonal antibody at 1:500 dilutionLane1:MCF-7 whole cell lysate
Lane2:NIH-3T3 whole cell lysate
Lane3:PC12 whole cell lysate
Electrophoresis was performed on a SDS-PAGE gel. To determine SDS-PAGE gel concentration
Protein Target Info & Infographic
Gene/Protein Information For CYBA (Source: Uniprot.org, NCBI)
Gene Name
CYBA
Full Name
Cytochrome b-245 light chain
Weight
21.013kDa
Superfamily
p22phox family
Alternative Names
cytochrome b light chain; Cytochrome b(558) alpha chain; cytochrome b(558) alpha-subunit; cytochrome b, alpha polypeptide; cytochrome b-245 light chain; cytochrome b-245, alpha polypeptide; Cytochrome b558 subunit alpha; flavocytochrome b-558 alpha polypeptide; Neutrophil cytochrome b 22 kDa polypeptide; p22 phagocyte B-cytochrome; p22phox; p22-PHOX; Superoxide-generating NADPH oxidase light chain subunit CYBA CGD4, p22-PHOX cytochrome b-245 alpha chain cytochrome b-245 light chain|cytochrome b light chain|cytochrome b(558) alpha chain|cytochrome b(558) alpha-subunit|cytochrome b, alpha polypeptide|cytochrome b-245, alpha polypeptide|cytochrome b558 subunit alpha|flavocytochrome b-558 alpha polypeptide|neutrophil cytochrome b 22 kDa polypeptide|p22 phagocyte B-cytochrome|p22phox|superoxide-generating NADPH oxidase light chain subunit
*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".For more info on CYBA, check out the CYBA Infographic
We have 30,000+ of these available, one for each gene! Check them out.
In this infographic, you will see the following information for CYBA: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].
Specific Publications For Anti-p22-phox CYBA Antibody (A03781)
Hello CJ!
A03781 has been cited in 1 publications:
*The publications in this section are manually curated by our staff scientists. They may differ from Bioz's machine gathered results. Both are accurate. If you find a publication citing this product but is missing from this list, please let us know we will issue you a thank-you coupon.
NADPH oxidase 3-associated oxidative stress and caspase 3-dependent apoptosis in the cochleae of D-galactose-induced aged rats
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