Anti-p22-phox CYBA Antibody

Product Name

Anti-p22-phox CYBA Antibody

View all Cytochrome b245 alpha Antibodies

SKU/Catalog Number

A03781

Size

100ul

Form

Liquid

Description

Boster Bio Anti-p22-phox CYBA Antibody catalog # A03781. Tested in WB applications. This antibody reacts with Human,Mouse,Rat.

Storage & Handling

Store at -20°C for one year. For short term storage and frequent use, store at 4°C for up to one month. Avoid repeated freeze-thaw cycles.

Cite This Product

Anti-p22-phox CYBA Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # A03781)

Host

Rabbit

Contents

Rabbit IgG, 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2

Clonality

Polyclonal

Isotype

IgG

Immunogen

Synthesized peptide derived from human NU6M protein.

*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.

Reactive Species

A03781 is reactive to CYBA in Human, Mouse, Rat

Applications

A03781 is guaranteed for WB Boster Guarantee

Observed Molecular Weight

Calculated molecular weight

21.013kDa

Background of Cytochrome b245 alpha

May catalyze alpha-1,3 and alpha-1,4 glycosidic linkages involved in the expression of Vim-2, Lewis A, Lewis B, sialyl Lewis X and Lewis X/SSEA-1 antigens. May be involved in blood group Lewis determination; Lewis-positive (Le+) individuals have an active enzyme while Lewis-negative (Le-) individuals have an inactive enzyme. Also acts on the corresponding 1,4-galactosyl derivative, forming 1,3-L-fucosyl links.

Kukowska-Latallo J.F., Genes Dev. 4:1288-1303(1990).
Cameron H.S., J. Biol. Chem. 270:20112-20122(1995).
Rahim I., Submitted (FEB-1999) to the EMBL/GenBank/DDBJ databases.

Antibody Validation

Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.

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Assay Dilutions Recommendation

The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.

WB: 1:500-1:1000

Validation Images & Assay Conditions

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Figure 1. Western blotting validation for Anti-p22-phox CYBA Antibody A03781

Western blot (WB) analysis of p22-phox polyclonal antibody at 1:500 dilution
Lane1:MCF-7 whole cell lysate
Lane2:NIH-3T3 whole cell lysate
Lane3:PC12 whole cell lysate
Electrophoresis was performed on a SDS-PAGE gel. To determine SDS-PAGE gel concentration

Gene/Protein Information For CYBA (Source: Uniprot.org, NCBI)

Alternative Names

cytochrome b light chain; Cytochrome b(558) alpha chain; cytochrome b(558) alpha-subunit; cytochrome b, alpha polypeptide; cytochrome b-245 light chain; cytochrome b-245, alpha polypeptide; Cytochrome b558 subunit alpha; flavocytochrome b-558 alpha polypeptide; Neutrophil cytochrome b 22 kDa polypeptide; p22 phagocyte B-cytochrome; p22phox; p22-PHOX; Superoxide-generating NADPH oxidase light chain subunit CYBA CGD4, p22-PHOX cytochrome b-245 alpha chain cytochrome b-245 light chain|cytochrome b light chain|cytochrome b(558) alpha chain|cytochrome b(558) alpha-subunit|cytochrome b, alpha polypeptide|cytochrome b-245, alpha polypeptide|cytochrome b558 subunit alpha|flavocytochrome b-558 alpha polypeptide|neutrophil cytochrome b 22 kDa polypeptide|p22 phagocyte B-cytochrome|p22phox|superoxide-generating NADPH oxidase light chain subunit

For more info on CYBA, check out the CYBA Infographic

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In this infographic, you will see the following information for CYBA: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].

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