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Product Info Summary
| SKU: | A01136-1 |
|---|---|
| Size: | 100 μg/vial |
| Reactive Species: | Mouse, Rat |
| Host: | Rabbit |
| Application: | Flow Cytometry, WB |
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Product info
Product Name
Anti-P2Y12/P2ry12 Antibody Picoband®
View all P2Y12/P2RY12 Antibodies
SKU/Catalog Number
A01136-1
Size
100 μg/vial
Form
Lyophilized
Description
Boster Bio Anti-P2Y12/P2ry12 Antibody Picoband® catalog # A01136-1. Tested in Flow Cytometry, WB applications. This antibody reacts with Mouse, Rat. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.
Storage & Handling
At -20°C for one year from date of receipt. After reconstitution, at 4°C for one month. It can also be aliquotted and stored frozen at -20°C for six months. Avoid repeated freezing and thawing.
Cite This Product
Anti-P2Y12/P2ry12 Antibody Picoband® (Boster Biological Technology, Pleasanton CA, USA, Catalog # A01136-1)
Host
Rabbit
Contents
Each vial contains 4 mg Trehalose, 0.9 mg NaCl, 0.2 mg Na2HPO4.
Clonality
Polyclonal
Isotype
Rabbit IgG
Immunogen
A synthetic peptide corresponding to a sequence in the middle region of mouse P2Y12/P2ry12, which shares 73.3% and 93.3% amino acid (aa) sequence identity with human and rat P2Y12/P2ry12, respectively.
*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.
Cross-reactivity
No cross-reactivity with other proteins.
Reactive Species
A01136-1 is reactive to P2ry12 in Mouse, Rat
Reconstitution
Adding 0.2 ml of distilled water will yield a concentration of 500 μg/ml.
Observed Molecular Weight
40 kDa
Calculated molecular weight
47643 MW
Background of P2Y12/P2RY12
P2Y12 is a chemoreceptor for adenosine diphosphate (ADP) that belongs to the Gi class of a group of G protein-coupled (GPCR) purinergic receptors. The product of this gene belongs to the family of G-protein coupled receptors. This family has several receptor subtypes with different pharmacological selectivity, which overlaps in some cases, for various adenosine and uridine nucleotides. This receptor is involved in platelet aggregation, and is a potential target for the treatment of thromboembolisms and other clotting disorders. Mutations in this gene are implicated in bleeding disorder, platelet type 8 (BDPLT8). Alternative splicing results in multiple transcript variants of this gene.
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
A01136-1 is guaranteed for Flow Cytometry, WB Boster Guarantee
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
Western blot, 0.25-0.5 μg/ml, Mouse, Rat
Flow Cytometry (Fixed), 1-3 μg/1x106 cells, Mouse
Positive Control
WB: rat brain tissue, rat brain tissue, mouse brain tissue, mouse brain tissue
FCM: HEPA1-6 cell
Validation Images & Assay Conditions
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Figure 1. Western blot analysis of P2Y12/P2ry12 using anti-P2Y12/P2ry12 antibody (A01136-1).
Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions.
Lane 1: rat brain tissue lysates,
Lane 2: rat brain tissue lysates,
Lane 3: mouse brain tissue lysates,
Lane 4: mouse brain tissue lysates.
After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-P2Y12/P2ry12 antigen affinity purified polyclonal antibody (Catalog # A01136-1) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for P2Y12/P2ry12 at approximately 40 kDa. The expected band size for P2Y12/P2ry12 is at 40 kDa.
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Figure 2. Flow Cytometry analysis of HEPA1-6 cells using anti-P2Y12/P2ry12 antibody (A01136-1).
Overlay histogram showing HEPA1-6 cells stained with A01136-1 (Blue line). The cells were fixed with 4% paraformaldehyde and blocked with 10% normal goat serum. And then incubated with rabbit anti-P2Y12/P2ry12 Antibody (A01136-1, 1 μg/1x106 cells) for 30 min at 20°C. DyLight®488 conjugated goat anti-rabbit IgG (BA1127, 5-10 μg/1x106 cells) was used as secondary antibody for 30 minutes at 20°C. Isotype control antibody (Green line) was rabbit IgG (1 μg/1x106) used under the same conditions. Unlabelled sample without incubation with primary antibody and secondary antibody (Red line) was used as a blank control.
Protein Target Info & Infographic
Gene/Protein Information For P2ry12 (Source: Uniprot.org, NCBI)
Gene Name
P2ry12
Full Name
P2Y purinoceptor 12
Weight
47643 MW
Superfamily
G-protein coupled receptor 1 family
Alternative Names
Alpha-galactosidase A;3.2.1.22 ;Alpha-D-galactosidase A;Alpha-D-galactoside galactohydrolase;Melibiase;Gla;Ags; P2ry12|P2y12|purinergic receptor P2Y12|P2Y purinoceptor 12|P2Y12 platelet ADP receptor|purinergic receptor P2Y, G-protein coupled, 12
*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".For more info on P2ry12, check out the P2ry12 Infographic
We have 30,000+ of these available, one for each gene! Check them out.
In this infographic, you will see the following information for P2ry12: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].
Specific Publications For Anti-P2Y12/P2ry12 Antibody Picoband® (A01136-1)
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