Anti-P2Y12/P2ry12 Antibody Picoband™

Product Name

Anti-P2Y12/P2ry12 Antibody Picoband™

View all P2Y12/P2RY12 Antibodies

SKU/Catalog Number

A01136-1

Size

100 μg/vial

Form

Lyophilized

Description

Boster Bio Anti-P2Y12/P2ry12 Antibody Picoband™ catalog # A01136-1. Tested in Flow Cytometry, WB applications. This antibody reacts with Mouse, Rat.

Storage & Handling

At -20°C for one year from date of receipt. After reconstitution, at 4°C for one month. It can also be aliquotted and stored frozen at -20°C for six months. Avoid repeated freezing and thawing.

Cite This Product

Anti-P2Y12/P2ry12 Antibody Picoband™ (Boster Biological Technology, Pleasanton CA, USA, Catalog # A01136-1)

Host

Rabbit

Contents

Each vial contains 4 mg Trehalose, 0.9 mg NaCl, 0.2 mg Na2HPO4.

Clonality

Polyclonal

Isotype

Rabbit IgG

Immunogen

A synthetic peptide corresponding to a sequence in the middle region of mouse P2Y12/P2ry12, which shares 73.3% and 93.3% amino acid (aa) sequence identity with human and rat P2Y12/P2ry12, respectively.

*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.

Cross-reactivity

No cross-reactivity with other proteins.

Reactive Species

A01136-1 is reactive to P2ry12 in Mouse, Rat

Applications

A01136-1 is guaranteed for Flow Cytometry, WB Boster Guarantee

Observed Molecular Weight

40 kDa

Calculated molecular weight

39.047kDa

Background of P2Y12/P2RY12

P2Y12 is a chemoreceptor for adenosine diphosphate (ADP) that belongs to the Gi class of a group of G protein-coupled (GPCR) purinergic receptors. The product of this gene belongs to the family of G-protein coupled receptors. This family has several receptor subtypes with different pharmacological selectivity, which overlaps in some cases, for various adenosine and uridine nucleotides. This receptor is involved in platelet aggregation, and is a potential target for the treatment of thromboembolisms and other clotting disorders. Mutations in this gene are implicated in bleeding disorder, platelet type 8 (BDPLT8). Alternative splicing results in multiple transcript variants of this gene.

Antibody Validation

Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.

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Reconsitution

Adding 0.2 ml of distilled water will yield a concentration of 500 μg/ml.

Assay Dilutions Recommendation

The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.

Western blot, 0.25-0.5 μg/ml, Mouse, Rat
Flow Cytometry, 1-3 μg/1x10⁶ cells, Mouse

Validation Images & Assay Conditions

Click image to see more details

Figure 1. Western blot analysis of P2Y12/P2ry12 using anti-P2Y12/P2ry12 antibody (A01136-1).
Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions.
Lane 1: rat brain tissue lysates,
Lane 2: rat brain tissue lysates,
Lane 3: mouse brain tissue lysates,
Lane 4: mouse brain tissue lysates.
After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-P2Y12/P2ry12 antigen affinity purified polyclonal antibody (Catalog # A01136-1) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for P2Y12/P2ry12 at approximately 40 kDa. The expected band size for P2Y12/P2ry12 is at 40 kDa.

Click image to see more details

Figure 2. Flow Cytometry analysis of HEPA1-6 cells using anti-P2Y12/P2ry12 antibody (A01136-1).
Overlay histogram showing HEPA1-6 cells stained with A01136-1 (Blue line). The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-P2Y12/P2ry12 Antibody (A01136-1, 1 μg/1x10⁶ cells) for 30 min at 20°C. DyLight®488 conjugated goat anti-rabbit IgG (BA1127, 5-10 μg/1x10⁶ cells) was used as secondary antibody for 30 minutes at 20°C. Isotype control antibody (Green line) was rabbit IgG (1 μg/1x10⁶) used under the same conditions. Unlabelled sample (Red line) was also used as a control.

Gene/Protein Information For P2ry12 (Source: Uniprot.org, NCBI)

Alternative Names

ADPG-R; Gi-coupled ADP receptor HORK3; HORK3; HORK3P2Y12 platelet ADP receptor; P2RY12; P2T(AC); P2Y purinoceptor 12; P2Y(AC); P2Y(ADP); P2Y(cyc); P2Y12; P2Y12ADP-glucose receptor; purinergic receptor P2RY12; purinergic receptor P2Y, G-protein coupled, 12; putative G-protein coupled receptor; SP1999; SP1999G-protein coupled receptor SP1999 P2ry12|P2y12|purinergic receptor P2Y12|P2Y purinoceptor 12|P2Y12 platelet ADP receptor|purinergic receptor P2Y, G-protein coupled, 12

For more info on P2ry12, check out the P2ry12 Infographic

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In this infographic, you will see the following information for P2ry12: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].

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