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Product Info Summary
| SKU: | PB9954 |
|---|---|
| Size: | 100 μg/vial |
| Reactive Species: | Human |
| Host: | Rabbit |
| Application: | IHC, WB |
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Product info
Product Name
Anti-MGP Antibody Picoband®
SKU/Catalog Number
PB9954
Size
100 μg/vial
Form
Lyophilized
Description
Boster Bio Anti-MGP Antibody Picoband® catalog # PB9954. Tested in IHC, WB applications. This antibody reacts with Human. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.
Storage & Handling
Store at -20˚C for one year from date of receipt. After reconstitution, at 4˚C for one month. It can also be aliquotted and stored frozen at -20˚C for six months. Avoid repeated freeze-thaw cycles.
Cite This Product
Anti-MGP Antibody Picoband® (Boster Biological Technology, Pleasanton CA, USA, Catalog # PB9954)
Host
Rabbit
Contents
Each vial contains antibody formulated with stabilizing components, 0.9 mg NaCl, 0.2 mg Na2HPO4, and 0.05 mg NaN3.
*This antibody is supplied in a stabilized formulation.
Compatibility with conjugation reactions depends on the chemistry of the conjugation method used.
For conjugation methods that are not compatible with the stabilizing components present in this formulation, a carrier-free antibody format is required.
Clonality
Polyclonal
Isotype
Rabbit IgG
Immunogen
E.coli-derived human MGP recombinant protein (Position: Y20-F96). Human MGP shares 84.4% and 80.5% amino acid (aa) sequence identity with mouse and rat MGP, respectively.
Cross-reactivity
No cross-reactivity with other proteins
Reactive Species
PB9954 is reactive to MGP in Human
Observed Molecular Weight
12 kDa
Calculated molecular weight
12.4 kDa
Background of MGP
Matrix Gla protein (MGP) is an 84-residue vitamin K-dependent protein initially isolated from bovine bone. In addition, MGP is a 10-kD protein produced and secreted by vascular smooth muscle cells and chondrocytes and significantly accumulated in bone, cartilage, and dentin. It is also expressed at high levels in heart, kidney, and lung and is upregulated by vitamin D in bone cells. MGP has a high affinity binding to calcium ions, similar to other Gla-containing proteins. The protein acts as an inhibitor of vascular mineralization and plays a role in bone organization. And this gene is mapped to 12p12.3.
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
PB9954 is guaranteed for IHC, WB Boster Guarantee
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
Immunohistochemistry (Paraffin-embedded Section), 0.5-1μg/ml, Human
Western blot, 0.1-0.5μg/ml, Human
Positive Control
WB: JURKAT whole cell
IHC: human lung cancer tissue
Validation Images & Assay Conditions
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Western blot analysis of MGP using anti-MGP antibody (PB9954).
Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions.
Lane 1: JURKAT whole cell lysates.
After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-MGP antigen affinity purified polyclonal antibody (Catalog # PB9954) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for MGP at approximately 12 kDa. The expected band size for MGP is at 12 kDa.
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IHC analysis of MGP using anti-MGP antibody (PB9954).
MGP was detected in a paraffin-embedded section of human lung cancer tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1 μg/ml rabbit anti-MGP Antibody (PB9954) overnight at 4°C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC) (Catalog # SA1022) with DAB as the chromogen.
Specific Publications For Anti-MGP Antibody Picoband® (PB9954)
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Customer Q&As
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6 Customer Q&As for Anti-MGP Antibody Picoband®
Question
Is a blocking peptide available for product anti-MGP antibody (PB9954)?
Verified Customer
Verified customer
Asked: 2019-09-02
Answer
We do provide the blocking peptide for product anti-MGP antibody (PB9954). If you would like to place an order for it please contact support@bosterbio.com and make a special request.
Boster Scientific Support
Answered: 2019-09-02
Question
I see that the anti-MGP antibody PB9954 works with WB, what is the protocol used to produce the result images on the product page?
Verified Customer
Verified customer
Asked: 2018-11-27
Answer
You can find protocols for WB on the "support/technical resources" section of our navigation menu. If you have any further questions, please send an email to support@bosterbio.com
Boster Scientific Support
Answered: 2018-11-27
Question
We are currently using anti-MGP antibody PB9954 for human tissue, and we are content with the IHC results. The species of reactivity given in the datasheet says human. Is it possible that the antibody can work on goat tissues as well?
Verified Customer
Verified customer
Asked: 2018-08-09
Answer
The anti-MGP antibody (PB9954) has not been tested for cross reactivity specifically with goat tissues, but there is a good chance of cross reactivity. We have an innovator award program that if you test this antibody and show it works in goat you can get your next antibody for free. Please contact me if I can help you with anything.
Boster Scientific Support
Answered: 2018-08-09
Question
Will anti-MGP antibody PB9954 work on canine WB with brain?
Verified Customer
Verified customer
Asked: 2018-08-06
Answer
Our lab technicians have not tested anti-MGP antibody PB9954 on canine. You can run a BLAST between canine and the immunogen sequence of anti-MGP antibody PB9954 to see if they may cross-react. If the sequence homology is close, then you can perform a pilot test. Keep in mind that since we have not validated canine samples, this use of the antibody is not covered by our guarantee. However we have an innovator award program that if you test this antibody and show it works in canine brain in WB, you can get your next antibody for free.
Boster Scientific Support
Answered: 2018-08-06
Question
I have a question about product PB9954, anti-MGP antibody. I was wondering if it would be possible to conjugate this antibody with biotin. I would need it to be without BSA or sodium azide. I am planning on using a buffer exchange of sodium azide with PBS only. Would there be problems for me to conjugate the antibody and store it in -20 degrees in small aliquots?
M. Jones
Verified customer
Asked: 2018-06-05
Answer
It is not recommended storing this antibody with PBS buffer only in -20 degrees. If you want to store it in -20 degrees it is best to add some cryoprotectant like glycerol. If you want carrier free PB9954 anti-MGP antibody, we can provide it to you in a special formula with trehalose and/or glycerol. These molecules will not interfere with conjugation chemistry and provide a good level of protection for the antibody from degradation. Please be sure to specify this in your purchase order.
Boster Scientific Support
Answered: 2018-06-05
Question
Will anti-MGP antibody PB9954 work for WB with cervix?
Verified Customer
Verified customer
Asked: 2018-03-07
Answer
According to the expression profile of cervix, MGP is highly expressed in cervix. So, it is likely that anti-MGP antibody PB9954 will work for WB with cervix.
Boster Scientific Support
Answered: 2018-03-07
