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Product Info Summary
| SKU: | A09252 |
|---|---|
| Size: | 100μl |
| Reactive Species: | Human, Mouse, Rat |
| Host: | Rabbit |
| Application: | WB |
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Product info
Product Name
Anti-P2Y4 P2RY4 Antibody
View all P2Y4/P2RY4 Antibodies
SKU/Catalog Number
A09252
Size
100μl
Form
Liquid
Description
Boster Bio Anti-P2Y4 P2RY4 Antibody catalog # A09252. Tested in WB applications. This antibody reacts with Human, Mouse, Rat.
Storage & Handling
Store at -20°C for one year. For short term storage and frequent use, store at 4°C for up to one month. Avoid repeated freeze-thaw cycles.
Cite This Product
Anti-P2Y4 P2RY4 Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # A09252)
Host
Rabbit
Contents
Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.
Clonality
Polyclonal
Clone Number
3D5B1
Isotype
IgG
Immunogen
Synthesized peptide derived from the Internal region of human P2Y4. at AA rangle: 130-210
*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.
Cross-reactivity
No cross reactivity with other proteins.
Reactive Species
A09252 is reactive to P2RY4 in Human, Mouse, Rat
Applications
A09252 is guaranteed for WB Boster Guarantee
Observed Molecular Weight
39 kDa
Calculated molecular weight
40.963kDa
Background of P2Y4/P2RY4
APC11 is also known as Anaphase promoting complex subunit 11, APC11, Cyclosome subunit 11, Hepatocellular carcinoma associated RING finger protein, and HSPC214. APC11 is a component of the anaphase promoting complex/cyclosome (APC/C), a cell cycle-regulated E3 ubiquitin ligase that controls progression through mitosis and the G1 phase of the cell cycle. APC11 may function to recruit the E2 ubiquitin-conjugating enzymes to the complex. APC11 interacts with the cullin domain of ANAPC2 and also interacts with UBE2D2. APC11 shows both a cytoplasmic and nuclear localization. APC11 is expressed at high levels in skeletal muscle and heart; in moderate levels in brain, kidney, and liver; and at low levels in colon, thymus, spleen, small intestine, placenta, lung and peripheral blood leukocyte. APC11 is a member of the RING-type zinc finger family and is auto-ubiquitinylated.
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
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Assay dilution & Images
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
WB, 1:500-1:2000
Validation Images & Assay Conditions
Click image to see more details
Western Blot (WB) analysis of specific cells using P2Y4 Polyclonal antibody.
Protein Target Info & Infographic
Gene/Protein Information For P2RY4 (Source: Uniprot.org, NCBI)
Gene Name
P2RY4
Full Name
P2Y purinoceptor 4
Weight
40.963kDa
Superfamily
G-protein coupled receptor 1 family
Alternative Names
NRU; P2P; P2RY4; P2Y4; P2Y4P2Y purinoceptor 4; pyrimidinergic receptor P2Y, G-protein coupled, 4; UNRNRUUridine nucleotide receptor P2RY4 NRU, P2P, P2Y4, UNR pyrimidinergic receptor P2Y4 P2Y purinoceptor 4|pyrimidinergic receptor P2Y, G-protein coupled, 4|uridine nucleotide receptor
*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".For more info on P2RY4, check out the P2RY4 Infographic
We have 30,000+ of these available, one for each gene! Check them out.
In this infographic, you will see the following information for P2RY4: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].
Specific Publications For Anti-P2Y4 P2RY4 Antibody (A09252)
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No publications found for A09252
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Customer Reviews
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Customer Q&As
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Find answers in Q&As, reviews.
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4 Customer Q&As for Anti-P2Y4 P2RY4 Antibody
Question
Would anti-P2Y4 antibody A09252 work for WB with small intestine peyer's patch?
Verified Customer
Verified customer
Asked: 2020-04-29
Answer
According to the expression profile of small intestine peyer's patch, P2RY4 is highly expressed in small intestine peyer's patch. So, it is likely that anti-P2Y4 antibody A09252 will work for WB with small intestine peyer's patch.
Boster Scientific Support
Answered: 2020-04-29
Question
I see that the anti-P2Y4 antibody A09252 works with WB, what is the protocol used to produce the result images on the product page?
Verified Customer
Verified customer
Asked: 2019-12-19
Answer
You can find protocols for WB on the "support/technical resources" section of our navigation menu. If you have any further questions, please send an email to [email protected]
Boster Scientific Support
Answered: 2019-12-19
Question
We are currently using anti-P2Y4 antibody A09252 for human tissue, and we are happy with the WB results. The species of reactivity given in the datasheet says human, mouse, rat. Is it likely that the antibody can work on bovine tissues as well?
P. Li
Verified customer
Asked: 2017-01-25
Answer
The anti-P2Y4 antibody (A09252) has not been validated for cross reactivity specifically with bovine tissues, but there is a good chance of cross reactivity. We have an innovator award program that if you test this antibody and show it works in bovine you can get your next antibody for free. Please contact me if I can help you with anything.
Boster Scientific Support
Answered: 2017-01-25
Question
My question regarding product A09252, anti-P2Y4 antibody. I was wondering if it would be possible to conjugate this antibody with biotin. I would need it to be without BSA or sodium azide. I am planning on using a buffer exchange of sodium azide with PBS only. Would there be problems for me to conjugate the antibody and store it in -20 degrees in small aliquots?
S. Miller
Verified customer
Asked: 2013-01-21
Answer
It is not recommended storing this antibody with PBS buffer only in -20 degrees. If you want to store it in -20 degrees it is best to add some cryoprotectant like glycerol. If you want carrier free A09252 anti-P2Y4 antibody, we can provide it to you in a special formula with trehalose and/or glycerol. These molecules will not interfere with conjugation chemistry and provide a good level of protection for the antibody from degradation. Please be sure to specify this in your purchase order.
Boster Scientific Support
Answered: 2013-01-21
