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Product Info Summary
| SKU: | A09252 |
|---|---|
| Size: | 100μl |
| Reactive Species: | Human, Mouse, Rat |
| Host: | Rabbit |
| Application: | ELISA, IF, WB |
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Product info
Product Name
Anti-P2Y4 P2RY4 Antibody
SKU/Catalog Number
A09252
Size
100μl
Form
Liquid
Description
Boster Bio Anti-P2Y4 P2RY4 Antibody catalog # A09252. Tested in WB, IF, ELISA applications. This antibody reacts with Human, Mouse, Rat.
Storage & Handling
Store at -20°C for one year. For short term storage and frequent use, store at 4°C for up to one month. Avoid repeated freeze-thaw cycles.
Cite This Product
Anti-P2Y4 P2RY4 Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # A09252)
Host
Rabbit
Contents
Liquid in PBS containing 50% glycerol, 0.5% stabilizing protein and 0.02% sodium azide.
This antibody is supplied in a stabilized formulation.
Compatibility with conjugation reactions depends on the chemistry of the conjugation method used.
For conjugation methods that are not compatible with the stabilizing components present in this formulation, a carrier-free antibody format is required.
Clonality
Polyclonal
Isotype
IgG
Immunogen
The antiserum was produced against synthesized peptide derived from human P2RY4. AA range:159-208
Cross-reactivity
No cross reactivity with other proteins.
Reactive Species
A09252 is reactive to P2RY4 in Human, Mouse, Rat
Observed Molecular Weight
39 kDa
Calculated molecular weight
41.0 kDa
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
A09252 is guaranteed for ELISA, IF, WB Boster Guarantee
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
WB 1:500-1:2000
IF 1:200-1:1000
ELISA 1:10000
Validation Images & Assay Conditions
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Western blot analysis of the lysates from COLO205 cells using P2RY4 antibody.
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Western Blot analysis of various cells using P2Y4 Polyclonal Antibody diluted at 1:500
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Western blot analysis of lysates from HeLa cells, using P2RY4 Antibody. The lane on the right is blocked with the synthesized peptide.
Specific Publications For Anti-P2Y4 P2RY4 Antibody (A09252)
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Customer Q&As
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4 Customer Q&As for Anti-P2Y4 P2RY4 Antibody
Question
Would anti-P2Y4 antibody A09252 work for WB with small intestine peyer's patch?
Verified Customer
Verified customer
Asked: 2020-04-29
Answer
According to the expression profile of small intestine peyer's patch, P2RY4 is highly expressed in small intestine peyer's patch. So, it is likely that anti-P2Y4 antibody A09252 will work for WB with small intestine peyer's patch.
Boster Scientific Support
Answered: 2020-04-29
Question
I see that the anti-P2Y4 antibody A09252 works with WB, what is the protocol used to produce the result images on the product page?
Verified Customer
Verified customer
Asked: 2019-12-19
Answer
You can find protocols for WB on the "support/technical resources" section of our navigation menu. If you have any further questions, please send an email to support@bosterbio.com
Boster Scientific Support
Answered: 2019-12-19
Question
We are currently using anti-P2Y4 antibody A09252 for human tissue, and we are happy with the WB results. The species of reactivity given in the datasheet says human, mouse, rat. Is it likely that the antibody can work on bovine tissues as well?
P. Li
Verified customer
Asked: 2017-01-25
Answer
The anti-P2Y4 antibody (A09252) has not been validated for cross reactivity specifically with bovine tissues, but there is a good chance of cross reactivity. We have an innovator award program that if you test this antibody and show it works in bovine you can get your next antibody for free. Please contact me if I can help you with anything.
Boster Scientific Support
Answered: 2017-01-25
Question
My question regarding product A09252, anti-P2Y4 antibody. I was wondering if it would be possible to conjugate this antibody with biotin. I would need it to be without BSA or sodium azide. I am planning on using a buffer exchange of sodium azide with PBS only. Would there be problems for me to conjugate the antibody and store it in -20 degrees in small aliquots?
S. Miller
Verified customer
Asked: 2013-01-21
Answer
It is not recommended storing this antibody with PBS buffer only in -20 degrees. If you want to store it in -20 degrees it is best to add some cryoprotectant like glycerol. If you want carrier free A09252 anti-P2Y4 antibody, we can provide it to you in a special formula with trehalose and/or glycerol. These molecules will not interfere with conjugation chemistry and provide a good level of protection for the antibody from degradation. Please be sure to specify this in your purchase order.
Boster Scientific Support
Answered: 2013-01-21
