Anti-P2Y6/P2RY6 Antibody Picoband™

Product Name

Anti-P2Y6/P2RY6 Antibody Picoband™

View all P2Y6/P2RY6 Antibodies

SKU/Catalog Number

A10087-2

Size

100 μg/vial

Form

Lyophilized

Description

Boster Bio Anti-P2Y6/P2RY6 Antibody Picoband™ catalog # A10087-2. Tested in WB applications. This antibody reacts with Human.

Storage & Handling

At -20°C for one year from date of receipt. After reconstitution, at 4°C for one month. It can also be aliquotted and stored frozen at -20°C for six months. Avoid repeated freezing and thawing.

Cite This Product

Anti-P2Y6/P2RY6 Antibody Picoband™ (Boster Biological Technology, Pleasanton CA, USA, Catalog # A10087-2)

Host

Rabbit

Contents

Each vial contains 4 mg Trehalose, 0.9 mg NaCl, 0.2 mg Na2HPO4.

Clonality

Polyclonal

Isotype

Rabbit IgG

Immunogen

A synthetic peptide corresponding to a sequence at the C-terminusof human P2Y6/P2RY6, which shares 94.4% amino acid (aa) sequence identity with rat P2RY6.

*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.

Cross-reactivity

No cross-reactivity with other proteins

Reactive Species

A10087-2 is reactive to P2RY6 in Human

Applications

A10087-2 is guaranteed for WB Boster Guarantee

Observed Molecular Weight

45 kDa

Calculated molecular weight

36.429kDa

Background of P2Y6/P2RY6

P2Y purinoceptor 6 is a protein that in humans is encoded by the P2RY6 gene. The product of this gene belongs to the family of P2 receptors, which is activated by extracellular nucleotides and subdivided into P2X ligand-gated ion channels and P2Y G-protein coupled receptors. This family has several receptor subtypes with different pharmacological selectivity, which overlaps in some cases, for various adenosine and uridine nucleotides. This receptor, which is a G-protein coupled receptor, is responsive to UDP, partially responsive to UTP and ADP, and not responsive to ATP. It is proposed that this receptor mediates inflammatory responses. Alternative splicing results in multiple transcript variants that encode different protein isoforms.

Antibody Validation

Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.

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Reconsitution

Adding 0.2 ml of distilled water will yield a concentration of 500 μg/ml.

Assay Dilutions Recommendation

The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.

Western blot, 0.25-0.5 μg/ml, Human

Validation Images & Assay Conditions

Click image to see more details

Figure 1. Western blot analysis of P2Y6/P2RY6 using anti-P2Y6/P2RY6 antibody (A10087-2).
Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions.
Lane 1: human Hacat whole cell lysates,
Lane 2: human HepG2 whole cell lysates,
Lane 3: human MCF-7 whole cell lysates.
After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-P2Y6/P2RY6 antigen affinity purified polyclonal antibody (Catalog # A10087-2) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for P2Y6/P2RY6 at approximately 45 kDa. The expected band size for P2Y6/P2RY6 is at 36 kDa.

Gene/Protein Information For P2RY6 (Source: Uniprot.org, NCBI)

Alternative Names

G-coupled nucleotide receptor; MGC15335; P2 purinoceptor; P2RY6; P2Y6 receptor; P2Y6; P2Y6P2Y purinoceptor 6; pyrimidinergic receptor P2Y, G-protein coupled, 6 P2RY6 P2Y6 pyrimidinergic receptor P2Y6 P2Y purinoceptor 6|G-coupled nucleotide receptor|P2 purinoceptor|P2Y6 receptor|pyrimidinergic receptor P2Y, G-protein coupled, 6

For more info on P2RY6, check out the P2RY6 Infographic

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In this infographic, you will see the following information for P2RY6: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].

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