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Product Info Summary
| SKU: | A04132-1 |
|---|---|
| Size: | 100 μl/vial |
| Reactive Species: | Human |
| Host: | Rabbit |
| Application: | ELISA, IP, IF, IHC, ICC, WB |
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Product info
Product Name
Anti-p40/RABEPK Antibody
SKU/Catalog Number
A04132-1
Size
100 μl/vial
Form
Liquid
Description
Boster Bio Anti-p40/RABEPK Antibody catalog # A04132-1. Tested in WB, IHC, ICC, IF, IP, ELISA applications. This antibody reacts with Human.
Storage & Handling
12 months from date of receipt，-20℃ as supplied. 6 months 2 to 8℃ after reconstitution. Avoid repeated freezing and thawing.
Cite This Product
Anti-p40/RABEPK Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # A04132-1)
Host
Rabbit
Contents
500 μg/ml antibody with PBS, 0.02% NaN3, 1 mg stabilizing protein and 50% glycerol
*This antibody is supplied in a stabilized formulation.
Compatibility with conjugation reactions depends on the chemistry of the conjugation method used.
For conjugation methods that are not compatible with the stabilizing components present in this formulation, a carrier-free antibody format is required.
Clonality
Polyclonal
Immunogen
E.coli-derived human p40/RABEPK recombinant protein (Position: Q3-V370).
Reactive Species
A04132-1 is reactive to RABEPK in Human
Calculated molecular weight
40.6 kDa
Background of RABEPK
Rab9 GTPase is required for the transport of mannose 6-phosphate receptors from endosomes to the trans-Golgi network in living cells, and in an in vitro system that reconstitutes this process. P40 is an effector of Rab9 that interacts preferentially with the active form of Rab9. p40 does not interact with Rab7 or K-Ras; it also fails to bind Rab9 when it is bound to GDI. The protein is found in cytosol, yet a significant fraction (~30%) is associated with cellular membranes. P40 is a very potent transport factor in that the pure, recombinant protein can stimulate, significantly, an in vitro transport assay that measures transport of mannose 6-phosphate receptors from endosomes to the trans-Golgi network.
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
A04132-1 is guaranteed for ELISA, IP, IF, IHC, ICC, WB Boster Guarantee
Recommend Dilution
| Application | Dilution | Species |
|---|---|---|
| Western blot | 1:500-2000 | |
| Immunohistochemistry | 1:50-400 | |
| Immunocytochemistry/Immunofluorescence | 1:50-400 | |
| ImmunoPrecipitation | 1:250-300 | |
| ELISA | 1:100-1000 |
Tested application
Suggested blocking solution with 5% non-fat milk or BSA; (*)Recommended protein loading: 20-40 µg per lane
Validation Images & Assay Conditions
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Western blot analysis of p40/RABEPK using anti-p40/RABEPK antibody (A04132-1).
Electrophoresis was performed on a 10% SDS-PAGE gel at 80V (Stacking gel) / 120V (Resolving gel) for 2 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions.
Lane 1: human HepG2 whole cell lysates,
Lane 2: human Hela whole cell lysates,
Lane 3: human A549 whole cell lysates,
Lane 4: human U251 whole cell lysates,
Lane 5: mouse testis tissue lysates,
Lane 6: mouse brain tissue lysates.
After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-p40/RABEPK antigen affinity purified polyclonal antibody (A04132-1) at 1:1000 overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an ECL Plus Western Blotting Substrate (Catalog # AR1196-200) with Tanon 5200 system. A specific band was detected for p40/RABEPK at approximately 41 kDa. The expected band size for p40/RABEPK is at 41 kDa.
Specific Publications For Anti-p40/RABEPK Antibody (A04132-1)
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