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Product Info Summary
| SKU: | M04229-1 |
|---|---|
| Size: | 100 μl/vial |
| Reactive Species: | Human, Mouse, Rat |
| Host: | Rabbit |
| Application: | IF, IHC, ICC, WB |
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Product info
Product Name
Anti-p53 DINP1 Rabbit Monoclonal Antibody
SKU/Catalog Number
M04229-1
Size
100 μl/vial
Form
Liquid
Description
Boster Bio Anti-p53 DINP1 Rabbit Monoclonal Antibody catalog # M04229-1. Tested in WB, IHC, ICC/IF applications. This antibody reacts with Human, Mouse, Rat.
Storage & Handling
Store at -20°C for one year. For short term storage and frequent use, store at 4°C for up to one month. Avoid repeated freeze-thaw cycles.
Cite This Product
Anti-p53 DINP1 Rabbit Monoclonal Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # M04229-1)
Host
Rabbit
Contents
Rabbit IgG in stabilizing components, phosphate buffered saline, pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
*This antibody is supplied in a stabilized formulation.
Compatibility with conjugation reactions depends on the chemistry of the conjugation method used.
For conjugation methods that are not compatible with the stabilizing components present in this formulation, a carrier-free antibody format is required.
Clonality
Monoclonal
Clone Number
19T28
Isotype
IgG
Immunogen
A synthesized peptide derived from human p53 DINP1
Reactive Species
M04229-1 is reactive to TP53INP1 in Human, Mouse, Rat
Observed Molecular Weight
32 kDa
Calculated molecular weight
27.4 kDa
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
M04229-1 is guaranteed for IF, IHC, ICC, WB Boster Guarantee
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
WB 1:500-2000
IHC 1:50-200
ICC/IF 1:50-200
Positive Control
Suggested blocking solution with 5% non-fat milk or BSA; (*)Recommended protein loading: 20-40 µg per lane
Validation Images & Assay Conditions
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Western blot analysis of p53 DINP1 expression in (1) HepG2 cell lysate; (2) RAW 264.7 cell lysate; (3) PC12 cell lysate.
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(A–C) Cisatracurium (10 or 20 μM) increases the expression of p53 in CRC cells. (A) Assessment of p53 protein expression level in HTC116 cells. (B) Bar chart of quantified p53 protein expression in HTC 116 cells. (C) Bar chart of quantified p53 mRNA levels in HTC116 cells. Data are presented as Mean ± SEM ( n = 3). Statistical significant differences in protein and mRNA of colon cancer cells were observed [ ∗∗ p < 0.01 and ∗∗∗ p < 0.001 versus control (0)].
Index in PubMed under a CC BY license. PMID: 30108509
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(A–F) Cisatracurium inhibits metastatic ability of CRC in vivo . (A) Line graph of subcutaneous tumor volume. (B) Weight of subcutaneous tumors in grams. Data are expressed as mean tumor volume or weight ± SE. ∗ p < 0.05. (C–F) Representative densities of tumor viability and migration regulatory proteins (p53, p21 and CD1, SNAI-1, CALD1, E-Cadherin) in tumor tissue samples. β-Actin was used as internal control. The cluster bar chats in (D , F) indicates the levels of viability and migration regulatory proteins in the treatment group. Data are expressed as Mean ± SEM ( n = 3). ∗ p < 0.05, ∗∗ p < 0.01, ∗∗∗ p < 0.001 versus control.
Index in PubMed under a CC BY license. PMID: 30108509
Specific Publications For Anti-p53 DINP1 Rabbit Monoclonal Antibody (M04229-1)
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