Anti-p53AIP1 TP53AIP1 Antibody

Product Name

Anti-p53AIP1 TP53AIP1 Antibody

View all TP53AIP1 Antibodies

SKU/Catalog Number

A13037-1

Size

0.1 mg

Form

Liquid

Description

Boster Bio Anti-p53AIP1 TP53AIP1 Antibody (Catalog # A13037-1). Tested in ELISA, WB, ICC, IF applications. This antibody reacts with Human.

Storage & Handling

p53AIP1 antibody can be stored at 4°C for three months and -20°C, stable for up to one year. Avoid repeated freeze-thaw cycles. Antibodies should not be exposed to prolonged high temperatures.

Cite This Product

Anti-p53AIP1 TP53AIP1 Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # A13037-1)

Host

Rabbit

Contents

p53AIP1 Antibody is supplied in PBS containing 0.02% sodium azide.

Clonality

Polyclonal

Isotype

IgG

Immunogen

p53AIP1 antibody was raised against a 15 amino acid synthetic peptide from near the amino terminus of human p53AIP1. The immunogen is located within the first 50 amino acids of p53AIP1.

Reactive Species

A13037-1 is reactive to TP53AIP1 in Human

Observed Molecular Weight

68 kDa

Calculated molecular weight

12.9 kDa

Background of TP53AIP1

The p53 tumor-suppressor protein can induce apoptosis through transcriptional activation of several genes. One such protein p53AIP was initially identified through direct cloning of p53 binding sequences from human genomic DNA. Its expression is inducible by p53 following p53 phosphorylation on Ser-46, and ectopic expression of p53AIP leads to apoptotic cell death. Both the phosphorylation of p53 and the induction of p53AIP were blocked by inhibiting the expression of p53DINP1 by the introduction of antisense oligonucleotides to p53DINP1, suggesting that the apoptosis associated with p53AIP expression is regulated by p53DINP1. Finally, as adenovirus-mediated introduction of p53AIP has been shown to suppress tumor growth in vivo, it has been suggested that p53AIP gene transfer may become a useful strategy for the treatment of p53-resistant cancers. Three isoforms of p53AIP are known to exist; this antibody will detect all three.

Antibody Validation

Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.

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Applications

A13037-1 is guaranteed for ELISA, IF, ICC, WB Boster Guarantee

Assay Dilutions Recommendation

The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.

p53AIP1 antibody can be used for detection of p53AIP1 by Western blot at 4 - 8 μg/mL. Antibody can also be used for immunocytochemistry starting at 10 μg/mL. For immunofluorescence start at 20 μg/mL.
Antibody validated: Western Blot in human samples; Immunocytochemistry in human samples and Immunofluorescence in human samples. All other applications and species not yet tested. Optimal dilutions for each application should be determined by the researcher.

Validation Images & Assay Conditions

See full target information TP53AIP1

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