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Product Info Summary
| SKU: | A03055-1 |
|---|---|
| Size: | 0.1 mg |
| Reactive Species: | Human, Mouse, Rat |
| Host: | Rabbit |
| Application: | ELISA, IF, IHC-P, WB |
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Product info
Product Name
Anti-p53R2 RRM2B Antibody
SKU/Catalog Number
A03055-1
Size
0.1 mg
Form
Liquid
Description
Boster Bio Anti-p53R2 RRM2B Antibody (Catalog # A03055-1). Tested in ELISA, WB, IHC-P, IF applications. This antibody reacts with Human, Mouse, Rat.
Storage & Handling
p53R2 antibody can be stored at 4°C for three months and -20°C, stable for up to one year. Avoid repeated freeze-thaw cycles. Antibodies should not be exposed to prolonged high temperatures.
Cite This Product
Anti-p53R2 RRM2B Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # A03055-1)
Host
Rabbit
Contents
p53R2 Antibody is supplied in PBS containing 0.02% sodium azide.
Clonality
Polyclonal
Isotype
IgG
Immunogen
p53R2 antibody was raised against a 16 amino acid peptide near the amino terminus of human p53R2. The immunogen is located within the first 50 amino acids of p53R2.
Cross-reactivity
At least six isoforms of p53R2 are known to exist; this antibody will detect all but the largest isoform.
Reactive Species
A03055-1 is reactive to RRM2B in Human, Mouse, Rat
Observed Molecular Weight
68 kDa
Calculated molecular weight
40.7 kDa
Background of RRM2B
The p53 tumor-suppressor gene integrates numerous signals that control cell life and death. Several novel molecules involved in p53 signaling, including p53R2, Chk2, p53AIP1, Noxa, PIDD, and PID/MTA2, were recently discovered. p53R2 is a p53 inducible gene that contains a p53 binding sequence and encodes a subunit of the enzyme ribonucleotide reductase. p53R2 is induced by the reagents, ultraviolet and gamma-irradiation that cause DNA damages. The product of p53R2 gene is directly involved in the p53 checkpoint for repair of damaged DNA. The isoform of the p53 family member p73 also induces p53R2 expression. p53R2 is an important target of p53 for tumor suppression.
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
A03055-1 is guaranteed for ELISA, IF, IHC-P, WB Boster Guarantee
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
p53R2 antibody can be used for detection of p53R2 by Western blot at 1 μg/mL. Antibody can also be used for immunohistochemistry starting at 1 μg/mL. For immunofluorescence start at 20 μg/mL.
Antibody validated: Western Blot in mouse samples; Immunohistochemistry in human samples and Immunofluorescence in human samples. All other applications and species not yet tested. Optimal dilutions for each application should be determined by the researcher.
Validation Images & Assay Conditions
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Western blot analysis of p53R2 in 3T3 cell lysate with p53R2 antibody at 1 μg/mL in (A) the absence and (B) the presence of blocking peptide.
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Immunohistochemistry of p53R2 in human lung tissue with p53R2 antibody at 1 μg/mL.
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Immunofluorescence of p53R2 in Human Lung tissue with p53R2 antibody at 20 μg/mL.
Specific Publications For Anti-p53R2 RRM2B Antibody (A03055-1)
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