Product Info Summary
SKU: | M09194 |
---|---|
Size: | 100 μl/vial |
Reactive Species: | Human, Mouse |
Host: | Rabbit |
Application: | Flow Cytometry, IF, IHC, ICC, WB |
Customers Who Bought This Also Bought
Product info
Product Name
Anti-p66 alpha Rabbit Monoclonal Antibody
SKU/Catalog Number
M09194
Size
100 μl/vial
Form
Liquid
Description
Boster Bio Anti-p66 alpha Rabbit Monoclonal Antibody catalog # M09194. Tested in WB, IHC, ICC/IF, Flow Cytometry applications. This antibody reacts with Human, Mouse.
Storage & Handling
Store at -20°C for one year. For short term storage and frequent use, store at 4°C for up to one month. Avoid repeated freeze-thaw cycles.
Cite This Product
Anti-p66 alpha Rabbit Monoclonal Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # M09194)
Host
Rabbit
Contents
Rabbit IgG in phosphate buffered saline, pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol, 0.4-0.5mg/ml BSA.
Clonality
Monoclonal
Clone Number
26G33
Isotype
IgG
Immunogen
A synthesized peptide derived from human p66 alpha
*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.
Reactive Species
M09194 is reactive to GATAD2A in Human, Mouse
Applications
M09194 is guaranteed for Flow Cytometry, IF, IHC, ICC, WB Boster Guarantee
Observed Molecular Weight
68 kDa
Calculated molecular weight
68.063kDa
Background of p66 alpha
Putative transcription factor involved in pancreas development and function.
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Innovating Scientists Reward
If you are the first to review this product, or if you have results for a special sample, species or application this product is not validated in, share your results with us and receive product credits you can use towards any Boster products! Applicable to all scientists worldwide.
Submit A Review
Assay dilution & Images
Reconsitution
Restore with deionized water (or equivalent) for reconstitution volume of 1.0 mL
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
WB 1:500-1:2000
IHC 1:50-1:200
ICC/IF 1:50-1:200
FC 1:50
Validation Images & Assay Conditions
Click image to see more details
Figure 1. Western blot analysis of p66 alpha using anti-p66 alpha antibody (M09194).
Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions.
Lane 1: human K562 whole cell lysates,
Lane 2: human Daudi whole cell lysates,
Lane 3: human HL-60 whole cell lysates,
Lane 7: mouse thymus tissue lysates.
After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with mouse anti-p66 alpha antigen affinity purified monoclonal antibody (Catalog # M09194) at 1:500 overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-mouse IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for p66 alpha at approximately 68 kDa. The expected band size for p66 alpha is at 68 kDa.
Protein Target Info & Infographic
Gene/Protein Information For GATAD2A (Source: Uniprot.org, NCBI)
Gene Name
GATAD2A
Full Name
Transcriptional repressor p66-alpha
Weight
68.063kDa
Alternative Names
FLJ20085; FLJ21017; GATA zinc finger domain containing 2A; GATA zinc finger domain-containing protein 2A; hp66alpha; p66 alpha; p66alpha; transcriptional repressor p66-alpha GATAD2A p66alpha GATA zinc finger domain containing 2A transcriptional repressor p66-alpha|GATA zinc finger domain-containing protein 2A
*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".For more info on GATAD2A, check out the GATAD2A Infographic
We have 30,000+ of these available, one for each gene! Check them out.
In this infographic, you will see the following information for GATAD2A: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].
Specific Publications For Anti-p66 alpha Rabbit Monoclonal Antibody (M09194)
Hello CJ!
No publications found for M09194
*Do you have publications using this product? Share with us and receive a reward. Ask us for more details.
Recommended Resources
Here are featured tools and databases that you might find useful.
- Boster's Pathways Library
- Protein Databases
- Bioscience Research Protocol Resources
- Data Processing & Analysis Software
- Photo Editing Software
- Scientific Literature Resources
- Research Paper Management Tools
- Molecular Biology Software
- Primer Design Tools
- Bioinformatics Tools
- Phylogenetic Tree Analysis
Customer Reviews
Have you used Anti-p66 alpha Rabbit Monoclonal Antibody?
Submit a review and receive an Amazon gift card.
- $30 for a review with an image
Be the first to review Anti-p66 alpha Rabbit Monoclonal Antibody
*The first user to submit a review for a product is eligible for Boster's Innovating Scientists Reward, which gives product credits. This is in addition to the gift card reward.
Customer Q&As
Have a question?
Find answers in Q&As, reviews.
Can't find your answer?
Submit your question