Product Info Summary
SKU: | M00732 |
---|---|
Size: | 100 μl |
Reactive Species: | Human, Mouse, Rat |
Host: | Rabbit |
Application: | Flow Cytometry, IP, IF, IHC, ICC, WB |
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Product info
Product Name
Anti-p95/NBS1 NBN Rabbit Monoclonal Antibody
SKU/Catalog Number
M00732
Size
100 μl
Form
Liquid
Description
Boster Bio Anti-p95/NBS1 NBN Rabbit Monoclonal Antibody catalog # M00732. Tested in WB, IHC, ICC/IF, IP, Flow Cytometry applications. This antibody reacts with Human, Mouse, Rat.
Storage & Handling
Store at -20°C for one year. For short term storage and frequent use, store at 4°C for up to one month. Avoid repeated freeze-thaw cycles.
Cite This Product
Anti-p95/NBS1 NBN Rabbit Monoclonal Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # M00732)
Host
Rabbit
Contents
Rabbit IgG in phosphate buffered saline, pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol, 0.4-0.5mg/ml BSA.
Clonality
Monoclonal
Clone Number
COF-14
Isotype
Rabbit IgG
Immunogen
A synthesized peptide derived from human p95/NBS1
*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.
Reactive Species
M00732 is reactive to NBN in Human, Mouse, Rat
Applications
M00732 is guaranteed for Flow Cytometry, IP, IF, IHC, ICC, WB Boster Guarantee
Observed Molecular Weight
95 kDa
Calculated molecular weight
84.959kDa
Background of Nbs1
Dynamin-related GTPase required for mitochondrial fusion and regulation of apoptosis. May form a diffusion barrier for proteins stored in mitochondrial cristae. Proteolytic processing in response to intrinsic apoptotic signals may lead to disassembly of OPA1 oligomers and release of the caspase activator cytochrome C (CYCS) into the mitochondrial intermembrane space.
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
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Assay dilution & Images
Reconsitution
Restore with deionized water (or equivalent) for reconstitution volume of 1.0 mL
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
WB 1:1000-1:2000
IHC 1:50-1:200
ICC/IF 1:50-1:200
IP 1:50
FC 1:50
Validation Images & Assay Conditions
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Figure 1. Western blot analysis of NBN using anti-NBN antibody (M00732).
Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions.
Lane 1: human Hela whole cell lysates,
Lane 2: human 293T whole cell lysates,
Lane 3: human K562 whole cell lysates.
After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-NBN antigen affinity purified monoclonal antibody (Catalog # M00732) at 1:1000 overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for NBN at approximately 95 kDa. The expected band size for NBN is at 85 kDa.
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Immunohistochemical analysis of paraffin-embedded Human glioblastoma, using the Antibody at 1:100 dilution.
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Immunohistochemical analysis of paraffin-embedded Rat kidney, using the Antibody at 1:100 dilution.
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Immunohistochemical analysis of paraffin-embedded Mouse heart, using the Antibody at 1:100 dilution.
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Immunohistochemical analysis of paraffin-embedded Rat heart, using the Antibody at 1:100 dilution.
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Immunohistochemical analysis of paraffin-embedded rat heart, using p95/NBS1 Antibody.
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Immunohistochemical analysis of paraffin-embedded Human lung large cell cancer, using the Antibody at 1:100 dilution.
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Immunofluorescent analysis using the Antibody at 1:50 dilution.
Protein Target Info & Infographic
Gene/Protein Information For NBN (Source: Uniprot.org, NCBI)
Gene Name
NBN
Full Name
Nibrin
Weight
84.959kDa
Alternative Names
ATV; AT-V1; AT-V2; Cell cycle regulatory protein p95; FLJ10155; MGC87362; NBN; Nbs1; NBS1P95; NBSNijmegen breakage syndrome protein 1; Nibrin; Nijmegen breakage syndrome 1 (nibrin); p95 protein of the MRE11/RAD50 complex; p95 NBN AT-V1, AT-V2, ATV, NBS, NBS1, P95 nibrin nibrin|Nijmegen breakage syndrome 1 (nibrin)|cell cycle regulatory protein p95|p95 protein of the MRE11/RAD50 complex
*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".For more info on NBN, check out the NBN Infographic
We have 30,000+ of these available, one for each gene! Check them out.
In this infographic, you will see the following information for NBN: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].
Specific Publications For Anti-p95/NBS1 NBN Rabbit Monoclonal Antibody (M00732)
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No publications found for M00732
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