Anti-PADI4/PAD4 Antibody

Product Name

Anti-PADI4/PAD4 Antibody

View all Padi4 Antibodies

SKU/Catalog Number

PA2043

Size

100 μg/vial

Form

Lyophilized

Description

Boster Bio Anti-PADI4/PAD4 Antibody catalog # PA2043. Tested in WB applications. This antibody reacts with Human.

Storage & Handling

Store at -20˚C for one year from date of receipt. After reconstitution, at 4˚C for one month. It can also be aliquotted and stored frozen at -20˚C for six months. Avoid repeated freeze-thaw cycles.

Cite This Product

Anti-PADI4/PAD4 Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # PA2043)

Host

Rabbit

Contents

Each vial contains 5mg BSA, 0.9mg NaCl, 0.2mg Na2HPO4, 0.05mg Thimerosal, 0.05mg NaN3.

Clonality

Polyclonal

Isotype

Rabbit IgG

Immunogen

A synthetic peptide corresponding to a sequence in the middle region of human PADI4.

*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.

Cross-reactivity

No cross-reactivity with other proteins

Reactive Species

PA2043 is reactive to Padi4 in Human

Applications

PA2043 is guaranteed for WB Boster Guarantee

Observed Molecular Weight

80 kDa

Calculated molecular weight

74.415kDa

Background of Padi4

PADI4 (Peptidylarginine Deiminase, Type IV), also known as PAD or PAD4, is a human protein which in humans is encoded by the PADI4 gene. Peptidylarginine deiminases, such as PADI4, make up a family of posttranslational modification enzymes that convert arginine residues to citrulline residues in the presence of calcium ion (Nakashima et al., 1999). Suzuki et al. (2003) found from sequence data that the PADI4 gene maps to 1p36. Cuthbert et al. (2004) described a process, deimination, that converts histone arginine to citrulline and antagonizes arginine methylation. They showed that PADI4 specifically deiminated arg2, arg8, arg17, and arg26 in the H3 tail. Deimination by PADI4 prevented arginine methylation by CARM1. Dimethylation of arginines prevented deimination by PADI4, although monomethylation still allowed deimination to take place. In vivo targeting experiments on an endogenous promoter demonstrated that PADI4 could repress hormone receptor-mediated gene induction. PADI4 was recruited to the pS2 promoter following hormone induction when the gene was transcriptionally downregulated, consistent with a repressive role for PADI4.

Antibody Validation

Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.

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Reconsitution

Add 0.2ml of distilled water will yield a concentration of 500ug/ml.

Assay Dilutions Recommendation

The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.

Western blot, 0.1-0.5μg/ml, Human

Validation Images & Assay Conditions

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Anti-PADI4/PAD4 antibody, PA2043, Western blotting
Lane 1: PANC Cell Lysate
Lane 2: 293T Cell Lysate

Gene/Protein Information For Padi4 (Source: Uniprot.org, NCBI)

Alternative Names

HL-60 PAD; PAD; PAD4; PADI4; PADI5PADI-H protein; PADPAD4; PDI4; PDI5EC 3.5.3.15; peptidyl arginine deiminase, type IV; peptidyl arginine deiminase, type V; Peptidylarginine deiminase IV; Protein-arginine deiminase type IV; protein-arginine deiminase type-4 Padi4|Pa, Pad4, Pd, Pdi4|peptidyl arginine deiminase, type IV|protein-arginine deiminase type-4|PAD type IV|peptidylarginine deiminase IV|protein-arginine deiminase type IV

For more info on Padi4, check out the Padi4 Infographic

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In this infographic, you will see the following information for Padi4: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].

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