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Product Info Summary
| SKU: | A12489 |
|---|---|
| Size: | 100μl |
| Reactive Species: | Human, Mouse |
| Host: | Rabbit |
| Application: | ELISA, IF, IHC, WB |
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Product info
Product Name
Anti-PAF65 alpha Antibody
SKU/Catalog Number
A12489
Size
100μl
Form
Liquid
Description
Boster Bio Anti-PAF65 alpha Antibody catalog # A12489. Tested in WB, IHC, IF, ELISA applications. This antibody reacts with Human, Mouse.
Storage & Handling
Store at -20°C for one year. For short term storage and frequent use, store at 4°C for up to one month. Avoid repeated freeze-thaw cycles.
Cite This Product
Anti-PAF65 alpha Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # A12489)
Host
Rabbit
Contents
Liquid in PBS containing 50% glycerol, 0.5% stabilizing protein and 0.02% sodium azide.
This antibody is supplied in a stabilized formulation.
Compatibility with conjugation reactions depends on the chemistry of the conjugation method used.
For conjugation methods that are not compatible with the stabilizing components present in this formulation, a carrier-free antibody format is required.
Clonality
Polyclonal
Isotype
IgG
Immunogen
The antiserum was produced against synthesized peptide derived from human TAF6L. AA range:31-80
Reactive Species
A12489 is reactive to TAF6L in Human, Mouse
Calculated molecular weight
67.8 kDa
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
A12489 is guaranteed for ELISA, IF, IHC, WB Boster Guarantee
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
WB 1:500-1:2000
IHC 1:100-1:300
ELISA 1:40000
IF 1:50-200
Validation Images & Assay Conditions
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Immunohistochemical analysis of paraffin-embedded human Colon cancer. 1, Antibody was diluted at 1:200 (4° overnight). 2, Tris-EDTA,pH9.0 was used for antigen retrieval. 3,Secondary antibody was diluted at 1:200 (room temperature, 45min).
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Western Blot analysis of 293 cells using PAF65α Polyclonal Antibody diluted at 1:1000 cells nucleus extracted by Minute TM Cytoplasmic and Nuclear Fractionation kit .
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Western blot analysis of lysates from 293 cells, using TAF6L Antibody. The lane on the right is blocked with the synthesized peptide.
Specific Publications For Anti-PAF65 alpha Antibody (A12489)
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Customer Q&As
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7 Customer Q&As for Anti-PAF65 alpha Antibody
Question
Will A12489 anti-PAF65 alpha antibody work on parafin embedded sections? If so, which fixation method do you recommend we use (PFA, paraformaldehyde, other)?
Verified Customer
Verified customer
Asked: 2019-10-25
Answer
It shows on the product datasheet, A12489 anti-PAF65 alpha antibody as been validated on WB. It is best to use PFA for fixation because it has better tissue penetration ability. PFA needs to be prepared fresh before use. Long term stored PFA turns into formalin, as the PFA molecules congregate and become formalin.
Boster Scientific Support
Answered: 2019-10-25
Question
Is there a BSA free version of anti-PAF65 alpha antibody A12489 available?
Verified Customer
Verified customer
Asked: 2019-08-05
Answer
I appreciate your recent telephone inquiry. I can confirm that some lots of this anti-PAF65 alpha antibody A12489 are BSA free. For now, these lots are available and we can make a BSA free formula for you free of charge. It will take 3 extra days to prepare. If you require this antibody BSA free again in future, please do not hesitate to contact me and I will be pleased to check which lots we have in stock that are BSA free.
Boster Scientific Support
Answered: 2019-08-05
Question
Is a blocking peptide available for product anti-PAF65 alpha antibody (A12489)?
Verified Customer
Verified customer
Asked: 2019-07-22
Answer
We do provide the blocking peptide for product anti-PAF65 alpha antibody (A12489). If you would like to place an order for it please contact support@bosterbio.com and make a special request.
Boster Scientific Support
Answered: 2019-07-22
Question
We are currently using anti-PAF65 alpha antibody A12489 for mouse tissue, and we are satisfied with the WB results. The species of reactivity given in the datasheet says human, mouse. Is it true that the antibody can work on primate tissues as well?
Verified Customer
Verified customer
Asked: 2019-05-29
Answer
The anti-PAF65 alpha antibody (A12489) has not been tested for cross reactivity specifically with primate tissues, but there is a good chance of cross reactivity. We have an innovator award program that if you test this antibody and show it works in primate you can get your next antibody for free. Please contact me if I can help you with anything.
Boster Scientific Support
Answered: 2019-05-29
Question
Can you help my question with product A12489, anti-PAF65 alpha antibody. I was wondering if it would be possible to conjugate this antibody with biotin. I would need it to be without BSA or sodium azide. I am planning on using a buffer exchange of sodium azide with PBS only. Would there be problems for me to conjugate the antibody and store it in -20 degrees in small aliquots?
Verified Customer
Verified customer
Asked: 2019-04-18
Answer
We do not recommend storing this antibody with PBS buffer only in -20 degrees. If you want to store it in -20 degrees it is best to add some cryoprotectant like glycerol. If you want carrier free A12489 anti-PAF65 alpha antibody, we can provide it to you in a special formula with trehalose and/or glycerol. These molecules will not interfere with conjugation chemistry and provide a good level of protection for the antibody from degradation. Please be sure to specify this in your purchase order.
Boster Scientific Support
Answered: 2019-04-18
Question
Does anti-PAF65 alpha antibody A12489 work for WB with right lobe of liver?
Verified Customer
Verified customer
Asked: 2018-03-09
Answer
According to the expression profile of right lobe of liver, TAF6L is highly expressed in right lobe of liver. So, it is likely that anti-PAF65 alpha antibody A12489 will work for WB with right lobe of liver.
Boster Scientific Support
Answered: 2018-03-09
Question
I appreciate helping with my inquiry over the phone. Here are the WB image, lot number and protocol we used for right lobe of liver using anti-PAF65 alpha antibody A12489. Let me know if you need anything else.
Verified Customer
Verified customer
Asked: 2017-07-25
Answer
We appreciate the data. You have provided everything we needed. Our lab team are working to resolve your inquiry as quickly as possible, and we appreciate your patience and understanding! Please let me know if there is anything you need in the meantime.
Boster Scientific Support
Answered: 2017-07-25
