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Product Info Summary
| SKU: | A07632-2 |
|---|---|
| Size: | 100 μg/vial |
| Reactive Species: | Human |
| Host: | Rabbit |
| Application: | ELISA, Flow Cytometry, IHC, WB |
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Product info
Product Name
Anti-R-Cadherin-4 CDH4 Antibody Picoband®
SKU/Catalog Number
A07632-2
Size
100 μg/vial
Form
Lyophilized
Description
Boster Bio Anti-R-Cadherin-4 CDH4 Antibody Picoband® catalog # A07632-2. Tested in ELISA, Flow Cytometry, IHC, WB applications. This antibody reacts with Human. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.
Storage & Handling
Store at -20˚C for one year from date of receipt. After reconstitution, at 4˚C for one month. It can also be aliquotted and stored frozen at -20˚C for six months. Avoid repeated freeze-thaw cycles.
Cite This Product
Anti-R-Cadherin-4 CDH4 Antibody Picoband® (Boster Biological Technology, Pleasanton CA, USA, Catalog # A07632-2)
Host
Rabbit
Contents
Each vial contains 4mg Trehalose, 0.9mg NaCl, 0.2mg Na2HPO4, 0.05mg NaN3.
Clonality
Polyclonal
Isotype
Rabbit IgG
Immunogen
E.coli-derived human R Cadherin/CDH4 recombinant protein (Position: K458-D641).
Cross-reactivity
No cross-reactivity with other proteins.
Reactive Species
A07632-2 is reactive to CDH4 in Human
Observed Molecular Weight
125 kDa
Calculated molecular weight
100.3 kDa
Background of CDH4
Cadherin-4 is a protein that in humans is encoded by the CDH4 gene. It is mapped to 20q13.33. This gene is a classical cadherin from the cadherin superfamily. The encoded protein is a calcium-dependent cell-cell adhesion glycoprotein comprised of five extracellular cadherin repeats, a transmembrane region and a highly conserved cytoplasmic tail. Based on studies in chicken and mouse, this cadherin is thought to play an important role during brain segmentation and neuronal outgrowth. In addition, a role in kidney and muscle development is indicated. Of particular interest are studies showing stable cis-heterodimers of cadherins 2 and 4 in cotransfected cell lines. Previously thought to interact in an exclusively homophilic manner, this is the first evidence of cadherin heterodimerization. Three transcript variants encoding different isoforms have been found for this gene.
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
A07632-2 is guaranteed for ELISA, Flow Cytometry, IHC, WB Boster Guarantee
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
Western blot, 0.1-0.5μg/ml
Immunohistochemistry (Paraffin-embedded Section), 0.5-1μg/ml
Flow Cytometry (Fixed), 1-3μg/1x106 cells
ELISA, 0.1-0.5μg/ml
Positive Control
WB: human U20S whole cell, human U87 whole cell, human A549 whole cell, human CACO-2 whole cell, human HepG2 whole cell, human K562 whole cell, human HELA whole cell
IHC: human glioma tissue
FCM: U20S cell
Validation Images & Assay Conditions
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Western blot analysis of R Cadherin/CDH4 using anti-R Cadherin/CDH4 antibody (A07632-2).
Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions.
Lane 1: human U20S whole cell lysates,
Lane 2: human U87 whole cell lysates,
Lane 3: human A549 whole cell lysates,
Lane 4: human CACO-2 whole cell lysates,
Lane 5: human HepG2 whole cell lysates,
Lane 6: human K562 whole cell lysates,
Lane 7: human HELA whole cell lysates.
After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-R Cadherin/CDH4 antigen affinity purified polyclonal antibody (Catalog # A07632-2) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for R Cadherin/CDH4 at approximately 125KD. The expected band size for R Cadherin/CDH4 is at 125KD.
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IHC analysis of R Cadherin/CDH4 using anti-R Cadherin/CDH4 antibody (A07632-2).
R Cadherin/CDH4 was detected in paraffin-embedded section of human glioma tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1μg/ml rabbit anti-R Cadherin/CDH4 Antibody (A07632-2) overnight at 4°C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC) (Catalog # SA1022) with DAB as the chromogen.
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Flow Cytometry analysis of U20S cells using anti-R Cadherin/CDH4 antibody (A07632-2).
Overlay histogram showing U20S cells stained with A07632-2 (Blue line). To facilitate intracellular staining, cells were fixed with 4% paraformaldehyde and permeabilized with permeabilization buffer. The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-R Cadherin/CDH4 Antibody (A07632-2, 1μg/1x106 cells) for 30 min at 20°C. DyLight®488 conjugated goat anti-rabbit IgG (BA1127, 5-10μg/1x106 cells) was used as secondary antibody for 30 minutes at 20°C. Isotype control antibody (Green line) was rabbit IgG (1μg/1x106) used under the same conditions. Unlabelled sample without incubation with primary antibody and secondary antibody (Red line) was used as a blank control.
Specific Publications For Anti-R-Cadherin-4 CDH4 Antibody Picoband® (A07632-2)
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