Product Info Summary
SKU: | M03637 |
---|---|
Size: | 100ul |
Reactive Species: | Human |
Host: | Mouse |
Application: | ELISA, IHC, WB |
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Product info
Product Name
Anti-PAR4 PAWR Monoclonal Antibody
SKU/Catalog Number
M03637
Size
100ul
Form
Liquid
Description
Boster Bio Anti-PAR4 PAWR Monoclonal Antibody catalog # M03637. Tested in ELISA, IHC, WB applications. This antibody reacts with Human.
Storage & Handling
Store at -20°C for one year. For short term storage and frequent use, store at 4°C for up to one month. Avoid repeated freeze-thaw cycles.
Cite This Product
Anti-PAR4 PAWR Monoclonal Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # M03637)
Host
Mouse
Contents
Ascitic fluid containing 0.03% sodium azide.
Clonality
Monoclonal
Clone Number
3G9H7 / 4H12E9
Isotype
IgG
Immunogen
Purified recombinant fragment of PAR4 (aa1-330) expressed in E. Coli.
*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.
Cross-reactivity
No cross reactivity with other proteins.
Reactive Species
M03637 is reactive to PAWR in Human
Applications
M03637 is guaranteed for ELISA, IHC, WB Boster Guarantee
Observed Molecular Weight
Calculated molecular weight
36.568kDa
Background of Pawr
Anti Mesothelin Antibody recognizes Mesothelin that is a glycosyl-phosphatidylinositol–anchored glycoprotein present on the cell surface of various human solid tumors. The mesothelin (MSLN) gene encodes a 71-kDa precursor protein that is processed to a 40-kDa glycosylphosphatidylinositol–anchored protein that composes the mature portion and an NH2 terminal 31-kDa fragment called megakaryocyte-potentiating factor that is released from the cell. Mesothelin is a tumor differentiation antigen present at low levels on a restricted set of normal adult tissues, such as mesothelium, but aberrantly over expressed in mesotheliomas, ovarian, and pancreatic cancers. The biological functions of mesothelin remain elusive. A recent study showed that mesothelin binds to MUC16/CA125, and that this interaction mediates cell adhesion, suggesting that there may be an important role for MUC16/CA125 and mesothelin in the metastatic spread of ovarian cancer.
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
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Assay dilution & Images
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
WB 1:500-1:2000
IHC 1:200-1:1000
ELISA 1:10000
Validation Images & Assay Conditions
Click image to see more details
Figure 2. Immunohistochemistry validation of PAWR using Anti-PAR4 PAWR Monoclonal Antibody (M03637).
Immunohistochemistry (IHC) analysis of paraffin-embedded Human Brain (left) and breast carcinoma (right)Click image to see more details
Figure 1. Western blotting validation for Anti-PAR4 PAWR Monoclonal Antibody M03637
Western Blot (WB) analysis using PAR4 Monoclonal Antibody against full-length Trx-PAR4 recombinant protein (1) and HeLa cell lysate (2).Electrophoresis was performed on a SDS-PAGE gel. To determine SDS-PAGE gel concentration
Protein Target Info & Infographic
Gene/Protein Information For PAWR (Source: Uniprot.org, NCBI)
Gene Name
PAWR
Full Name
PRKC apoptosis WT1 regulator protein
Weight
36.568kDa
Alternative Names
Par-4; PAR4prostate apoptosis response protein 4; PRKC apoptosis WT1 regulator protein; PRKC, apoptosis, WT1, regulator; Prostate apoptosis response 4 protein; prostate apoptosis response protein PAR-4; transcriptional repressor PAR4; WT1-interacting protein PAWR PAR4, Par-4 pro-apoptotic WT1 regulator PRKC apoptosis WT1 regulator protein|PRKC, apoptosis, WT1, regulator|WT1-interacting protein|prostate apoptosis response protein 4|prostate apoptosis response protein PAR-4|prostate apoptosis response-4|transcriptional repressor PAR4
*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".For more info on PAWR, check out the PAWR Infographic
We have 30,000+ of these available, one for each gene! Check them out.
In this infographic, you will see the following information for PAWR: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].
Specific Publications For Anti-PAR4 PAWR Monoclonal Antibody (M03637)
Hello CJ!
No publications found for M03637
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Customer Reviews
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