Anti-PARD3/Par3 Antibody

Product Name

Anti-PARD3/Par3 Antibody

View all PARD3/Par3 Antibodies

SKU/Catalog Number

A01897

Size

100 µl

Form

Liquid

Description

Boster Bio Anti-PARD3/Par3 Antibody (Catalog # A01897). Tested in WB, IF applications. This antibody reacts with Human, Mouse, Rat.

Storage & Handling

Store at -20°C for one year. For short term storage and frequent use, store at 4°C for up to one month. Avoid repeated freeze-thaw cycles.

Cite This Product

Anti-PARD3/Par3 Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # A01897)

Host

Rabbit

Contents

Rabbit IgG in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.

Clonality

Polyclonal

Isotype

IgG

Immunogen

Synthesized peptide derived from internal of human PARD3.

*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.

Cross-reactivity

No cross reactivity with other proteins.

Reactive Species

A01897 is reactive to PARD3 in Human, Mouse, Rat

Applications

A01897 is guaranteed for IF, WB Boster Guarantee

Observed Molecular Weight

Calculated molecular weight

151.423kDa

Background of PARD3/Par3

Adapter protein involved in asymmetrical cell division and cell polarization processes. Seems to play a central role in the formation of epithelial tight junctions. Targets the phosphatase PTEN to cell junctions By similarity. Association with PARD6B may prevent the interaction of PARD3 with F11R/JAM1, thereby preventing tight junction assembly. The PARD6-PARD3 complex links GTP-bound Rho small GTPases to atypical protein kinase C proteins. Required for establishment of neuronal polarity and normal axon formation in cultured hippocampal neurons.

Joberty G., Nat. Cell Biol. 2:531-539(2000).
Fang C.M., Cell Res. 11:223-229(2001).
Kohjima M., Biochem. Biophys. Res. Commun. 299:641-646(2002).

Antibody Validation

Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.

View more details

Innovating Scientists Reward

If you are the first to review this product, or if you have results for a special sample, species or application this product is not validated in, share your results with us and receive product credits you can use towards any Boster products! Applicable to all scientists worldwide.

Submit A Review

Assay Dilutions Recommendation

The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.

Western blotting: 1:500~1:3000

Immunofluorescence: 1:100~1:500

Validation Images & Assay Conditions

Click image to see more details

Western blot analysis of extracts from COLO205 cells, using PARD3 antibody A01897.

Click image to see more details

Immunofluorescence analysis of HepG2 cells, using PARD3 antibody A01897.

Gene/Protein Information For PARD3 (Source: Uniprot.org, NCBI)

Alternative Names

ASIP; Baz; Bazooka; FLJ21015; par-3 partitioning defective 3 homolog (C. elegans); PAR3; PAR3A; PAR3C.elegans) homolog; PARD3; SE2-5L16; SE2-5LT1; SE2-5T2 PARD3 ASIP, Baz, PAR3, PAR3alpha, PARD-3A, PPP1R118, SE2-5L16, SE2-5LT1, SE2-5T2, PARD3 par-3 family cell polarity regulator partitioning defective 3 homolog|CTCL tumor antigen se2-5|PAR3-alpha|atypical PKC isotype-specific interacting protein|bazooka|par-3 family cell polarity regulator alpha|par-3 partitioning defective 3 homolog|protein phosphatase 1, regulatory subunit 118

For more info on PARD3, check out the PARD3 Infographic

We have 30,000+ of these available, one for each gene! Check them out.

In this infographic, you will see the following information for PARD3: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].

Take me to the PARD3 Infographic

Here are featured tools and databases that you might find useful.

• Boster's Pathways Library
• Protein Databases
• Bioscience Research Protocol Resources
• Data Processing & Analysis Software
• Photo Editing Software
• Scientific Literature Resources
• Research Paper Management Tools
• Molecular Biology Software
• Primer Design Tools
• Bioinformatics Tools
• Phylogenetic Tree Analysis