Anti-PAX5/Bsap Rabbit Monoclonal Antibody

Product Name

Anti-PAX5/Bsap Rabbit Monoclonal Antibody

View all Pax5/BSAP Antibodies

SKU/Catalog Number

M00669

Size

100 μl

Form

Liquid

Description

Boster Bio Anti-PAX5/Bsap Rabbit Monoclonal Antibody catalog # M00669. Tested in WB, IHC, ICC/IF, Flow Cytometry applications. This antibody reacts with Human, Mouse.

Storage & Handling

Store at -20°C for one year. For short term storage and frequent use, store at 4°C for up to one month. Avoid repeated freeze-thaw cycles.

Cite This Product

Anti-PAX5/Bsap Rabbit Monoclonal Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # M00669)

Host

Rabbit

Contents

Rabbit IgG in phosphate buffered saline, pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol, 0.4-0.5mg/ml BSA.

Clonality

Monoclonal

Clone Number

ICD-16

Isotype

Rabbit IgG

Immunogen

A synthesized peptide derived from human PAX5

*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.

Reactive Species

M00669 is reactive to PAX5 in Human, Mouse

Applications

M00669 is guaranteed for Flow Cytometry, IF, IHC, ICC, WB Boster Guarantee

Observed Molecular Weight

54 kDa

Calculated molecular weight

42.149kDa

Background of Pax5/BSAP

Dynamin-related GTPase required for mitochondrial fusion and regulation of apoptosis. May form a diffusion barrier for proteins stored in mitochondrial cristae. Proteolytic processing in response to intrinsic apoptotic signals may lead to disassembly of OPA1 oligomers and release of the caspase activator cytochrome C (CYCS) into the mitochondrial intermembrane space.

Antibody Validation

Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.

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Innovating Scientists Reward

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Reconsitution

Restore with deionized water (or equivalent) for reconstitution volume of 1.0 mL

Assay Dilutions Recommendation

The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.

WB 1:1000-1:2000
IHC 1:50-1:200
ICC/IF 1:50-1:200
FC 1:30

Validation Images & Assay Conditions

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All lanes use the Antibody at 1:6K dilution for 1 hour at room temperature.

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Western blot analysis of PAX5 expression in Ramos cell lysate.

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All lanes use the Antibody at 1:6K dilution for 1 hour at room temperature.

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Immunohistochemical analysis of paraffin-embedded human tonsil, using PAX5 Antibody.

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Immunofluorescent analysis using the Antibody at 1:50 dilution.

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Immunofluorescent analysis of Ramos cells, using PAX5 Antibody.

Gene/Protein Information For PAX5 (Source: Uniprot.org, NCBI)

Alternative Names

B cell specific activator protein; B-cell-specific transcription factor; BSAP; BSAPB-cell lineage specific activator; paired box 5; paired box gene 5 (B-cell lineage specific activator protein); paired box gene 5 (B-cell lineage specific activator); paired box homeotic gene 5; paired box protein Pax-5; Pax5; transcription factor PAX 5 PAX5 ALL3, BSAP paired box 5 paired box protein Pax-5|B-cell lineage specific activator|paired box homeotic gene 5|paired domain gene 5|transcription factor PAX 5

For more info on PAX5, check out the PAX5 Infographic

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In this infographic, you will see the following information for PAX5: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].

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