Product Info Summary
SKU: | A10577-1 |
---|---|
Size: | 100 µg/vial |
Reactive Species: | Human, Mouse, Rat |
Host: | Rabbit |
Application: | ELISA, Flow Cytometry, IF, ICC, WB |
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Product info
Product Name
Anti-PCTP-L/STARD10 Antibody Picoband™
SKU/Catalog Number
A10577-1
Size
100 µg/vial
Form
Lyophilized
Description
Boster Bio Anti-PCTP-L/STARD10 Antibody Picoband™ catalog # A10577-1. Tested in ELISA, Flow Cytometry, IF, ICC, WB applications. This antibody reacts with Human, Mouse, Rat.
Storage & Handling
At -20°C for one year from date of receipt. After reconstitution, at 4°C for one month. It can also be aliquotted and stored frozen at -20°C for six months. Avoid repeated freezing and thawing.
Cite This Product
Anti-PCTP-L/STARD10 Antibody Picoband™ (Boster Biological Technology, Pleasanton CA, USA, Catalog # A10577-1)
Host
Rabbit
Contents
Each vial contains 4 mg Trehalose, 0.9 mg NaCl, 0.2 mg Na2HPO4.
Clonality
Polyclonal
Isotype
Rabbit IgG
Immunogen
E.coli-derived human PCTP-L/STARD10 recombinant protein (Position: M1-E273).
*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.
Cross-reactivity
No cross-reactivity with other proteins.
Reactive Species
A10577-1 is reactive to STARD10 in Human, Mouse, Rat
Applications
A10577-1 is guaranteed for ELISA, Flow Cytometry, IF, ICC, WB Boster Guarantee
Observed Molecular Weight
37 kDa
Calculated molecular weight
33.049kDa
Background of STARD10
StAR-related lipid transfer protein 10 (STARD10) or PCTP-like protein is a lipid transfer protein that in humans is encoded by the STARD10 gene. StarD10 is a dual-specificity lipid transfer protein capable of shuttling phosphatidylcholine (PC) and phosphatidylethanolamine (PE) between membranes in vitro; changes in membrane composition of PC and PE occur before the morphological tumorigenic events. Interestingly, STARD10 has been described to be highly expressed in 35-40% of ERBB2-positive breast cancers. StarD10 was initially discovered based on its cross-reactivity with a phosphoserine-specific antibody in mammary tumors from Neu/ErbB2 transgenic mice and subsequently isolated from SKBR3 human breast carcinoma cells using a multistep biochemical purification strategy. StarD10 contains a steroidogenic acute regulatory protein (StAR/StarD1)-related lipid transfer (START) domain that is thought to mediate binding of lipids. STARD10 is highly expressed in the liver and has been shown to transfer phosphatidylcholine.
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
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Assay dilution & Images
Reconsitution
Adding 0.2 ml of distilled water will yield a concentration of 500 µg/ml.
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
Western blot, 0.25-0.5 µg/ml, Human, Mouse, Rat
Immunocytochemistry/Immunofluorescence, 5 μg/ml, Human
Flow Cytometry(Fixed), 1-3 μg/1x106 cells, Human
Direct ELISA, 0.1-0.5 µg/ml, Human
Validation Images & Assay Conditions
Click image to see more details
Figure 1. Western blot analysis of PCTP-L/STARD10 using anti-PCTP-L/STARD10 antibody (A10577-1).
Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions.
Lane 1: human RT4 whole cell lysates,
Lane 2: human MCF-7 whole cell lysates,
Lane 3: human T47D whole cell lysates,
Lane 4: rat liver tissue lysates,
Lane 5: mouse liver tissue lysates,
Lane 6: mouse HEPA1-6 whole cell lysates.
After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-PCTP-L/STARD10 antigen affinity purified polyclonal antibody (Catalog # A10577-1) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for PCTP-L/STARD10 at approximately 37 kDa. The expected band size for PCTP-L/STARD10 is at 33 kDa.
Click image to see more details
Figure 2. IF analysis of PCTP-L/STARD10 using anti-PCTP-L/STARD10 antibody (A10577-1).
PCTP-L/STARD10 was detected in an immunocytochemical section of CACO-2 cells. Enzyme antigen retrieval was performed using IHC enzyme antigen retrieval reagent (AR0022) for 15 mins. The cells were blocked with 10% goat serum. And then incubated with 5 μg/mL rabbit anti-PCTP-L/STARD10 Antibody (A10577-1) overnight at 4°C. Cy3 Conjugated Goat Anti-Rabbit IgG (BA1032) was used as secondary antibody at 1:500 dilution and incubated for 30 minutes at 37°C. The section was counterstained with DAPI. Visualize using a fluorescence microscope and filter sets appropriate for the label used.
Click image to see more details
Figure 3. Flow Cytometry analysis of MCF-7 cells using anti-PCTP-L/STARD10 antibody (A10577-1).
Overlay histogram showing MCF-7 cells stained with A10577-1 (Blue line). To facilitate intracellular staining, cells were fixed with 4% paraformaldehyde and permeabilized with permeabilization buffer. The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-PCTP-L/STARD10 Antibody (A10577-1, 1 μg/1x106 cells) for 30 min at 20°C. DyLight®488 conjugated goat anti-rabbit IgG (BA1127, 5-10 μg/1x106 cells) was used as secondary antibody for 30 minutes at 20°C. Isotype control antibody (Green line) was rabbit IgG (1 μg/1x106) used under the same conditions. Unlabelled sample (Red line) was also used as a control.
Protein Target Info & Infographic
Gene/Protein Information For STARD10 (Source: Uniprot.org, NCBI)
Gene Name
STARD10
Full Name
START domain-containing protein 10
Weight
33.049kDa
Alternative Names
Antigen NY-CO-28; CGI-52; MGC14401; NY-CO-28; PCTP2; PCTP-L; PCTP-like protein; Serologically defined colon cancer antigen 28SDCCAG28; StARD10; StAR-related lipid transfer (START) domain containing 10; StAR-related lipid transfer protein 10; START domain containing 10; START domain-containing protein 10 STARD10 CGI-52, NY-CO-28, PCTP2, SDCCAG28 StAR related lipid transfer domain containing 10 START domain-containing protein 10|PCTP-L|PCTP-like protein|START domain containing 10|StAR-related lipid transfer (START) domain containing 10|antigen NY-CO-28|serologically defined colon cancer antigen 28|stAR-related lipid transfer protein 10
*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".For more info on STARD10, check out the STARD10 Infographic
We have 30,000+ of these available, one for each gene! Check them out.
In this infographic, you will see the following information for STARD10: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].
Specific Publications For Anti-PCTP-L/STARD10 Antibody Picoband™ (A10577-1)
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