Anti-PDCD10 Antibody Picoband™

Product Name

Anti-PDCD10 Antibody Picoband™

View all PDCD10 Antibodies

SKU/Catalog Number

A01879-1

Size

100 μg/vial

Form

Lyophilized

Description

Boster Bio Anti-PDCD10 Antibody Picoband™ catalog # A01879-1. Tested in ELISA, WB applications. This antibody reacts with Human, Mouse, Rat.

Storage & Handling

Store at -20˚C for one year from date of receipt. After reconstitution, at 4˚C for one month. It can also be aliquotted and stored frozen at -20˚C for six months. Avoid repeated freeze-thaw cycles.

Cite This Product

Anti-PDCD10 Antibody Picoband™ (Boster Biological Technology, Pleasanton CA, USA, Catalog # A01879-1)

Host

Rabbit

Contents

Each vial contains 4mg Trehalose, 0.9mg NaCl, 0.2mg Na₂HPO₄, 0.05mg NaN₃.

Clonality

Polyclonal

Isotype

Rabbit IgG

Immunogen

E. coli-derived human PDCD10 recombinant protein (Position: M1-A212).

*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.

Cross-reactivity

No cross-reactivity with other proteins.

Reactive Species

A01879-1 is reactive to PDCD10 in Human, Mouse, Rat

Applications

A01879-1 is guaranteed for ELISA, WB Boster Guarantee

Observed Molecular Weight

29 kDa

Calculated molecular weight

24.702kDa

Background of PDCD10

Programmed cell death protein 10 is a protein that in humans is encoded by the PDCD10 gene. This gene encodes an evolutionarily conserved protein associated with cell apoptosis. The protein interacts with the serine/threonine protein kinase MST4 to modulate the extracellular signal-regulated kinase (ERK) pathway. It also interacts with and is phosphoryated by serine/threonine kinase 25, and is thought to function in a signaling pathway essential for vascular developent. Mutations in this gene are one cause of cerebral cavernous malformations, which are vascular malformations that cause seizures and cerebral hemorrhages. Multiple alternatively spliced variants, encoding the same protein, have been identified.

Antibody Validation

Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.

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Reconsitution

Add 0.2ml of distilled water will yield a concentration of 500ug/ml.

Assay Dilutions Recommendation

The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.

Western blot, 0.1-0.5μg/ml
Direct ELISA, 0.1-0.5μg/ml

Validation Images & Assay Conditions

Click image to see more details

Figure 1. Western blot analysis of PDCD10 using anti-PDCD10 antibody (A01879-1).
Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions.
Lane 1: rat smooth muscle tissue lysates,
Lane 2: mouse smooth muscle tissue lysates.
After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-PDCD10 antigen affinity purified polyclonal antibody (Catalog # A01879-1) at 0.5 μg/mL overnight at 4℃, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for PDCD10 at approximately 29KD. The expected band size for PDCD10 is at 25KD.

Gene/Protein Information For PDCD10 (Source: Uniprot.org, NCBI)

Alternative Names

apoptosis-related protein 15; cerebral cavernous malformations 3; MGC1212; MGC24477; programmed cell death 10; programmed cell death protein 10; TF-1 cell apoptosis-related protein 15; TFAR15CCM3Cerebral cavernous malformations 3 protein PDCD10 CCM3, TFAR15 programmed cell death 10 programmed cell death protein 10|TF-1 cell apoptosis-related protein 15|apoptosis-related protein 15|cerebral cavernous malformations 3 protein

For more info on PDCD10, check out the PDCD10 Infographic

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In this infographic, you will see the following information for PDCD10: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].

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