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Product Info Summary
| SKU: | M01105 |
|---|---|
| Size: | 100 μl |
| Reactive Species: | Human, Mouse |
| Host: | Rabbit |
| Application: | Flow Cytometry, IF, IHC, ICC, WB |
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Product info
Product Name
Anti-PDCD4 Rabbit Monoclonal Antibody
SKU/Catalog Number
M01105
BM5208 is an alternative SKU for this antibody, used in previous lots.
Size
100 μl
Form
Liquid
Description
Boster Bio Anti-PDCD4 Rabbit Monoclonal Antibody catalog # M01105. Tested in WB, IHC, ICC/IF, Flow Cytometry applications. This antibody reacts with Human, Mouse.
Storage & Handling
Store at -20°C for one year. For short term storage and frequent use, store at 4°C for up to one month. Avoid repeated freeze-thaw cycles.
Cite This Product
Anti-PDCD4 Rabbit Monoclonal Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # M01105)
Host
Rabbit
Contents
Rabbit IgG in stabilizing components, phosphate buffered saline, pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
*This antibody is supplied in a stabilized formulation.
Compatibility with conjugation reactions depends on the chemistry of the conjugation method used.
For conjugation methods that are not compatible with the stabilizing components present in this formulation, a carrier-free antibody format is required.
Clonality
Monoclonal
Clone Number
ACAE-16
Isotype
Rabbit IgG
Immunogen
A synthesized peptide derived from human PDCD4
Reactive Species
M01105 is reactive to PDCD4 in Human, Mouse
Observed Molecular Weight
52 kDa
Calculated molecular weight
51.7 kDa
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
M01105 is guaranteed for Flow Cytometry, IF, IHC, ICC, WB Boster Guarantee
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
WB 1:500-2000
IHC 1:50-200
ICC/IF 1:50-200
FC 1:20
Validation Images & Assay Conditions
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All lanes use the Antibody at 1:2K dilution for 1 hour at room temperature.
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Andrographolide (Andro) inhibits breast cancer cell proliferation, migration, and invasion via NF-κB/miR-21-5p/PDCD4 signaling. (A) Heat map of the qRT-PCR data of miRNA expression in MCF-7 cells with or without NF-κB inhibition. APDC: NF-κB inhibitor. (B) miR-21-5p expression in breast cancer tissue and MCF cells was also detected using qRT-PCR. miR-21-5p was markedly suppressed by Andro in MCF-7 cells and MMTV-PyMT mice. (C) Colony formation, wound healing, Transwell migration, and Matrigel invasion assay. Immunohistochemistry (D) and Immunoblotting assay (E) for PDCD4 expression in DMSO- and Andro-treated MMTV-PyMT mice. (F) Immunoblotting analysis for PDCD4 expression in MCF-7 cells. (G) Schematic of the mechanisms of Andro-mediated effect on luminal-like breast cancer growth and metastasis. Quantitative data are expressed as the mean ± SD, n = 3. Significant effect: * P < 0.05, ** P < 0.01, *** P < 0.005. ns , no significant effect. Scale bar , 100 μM.
Index in PubMed under a CC BY license. PMID: 34249905
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Western blot analysis of PDCD4 expression in HeLa cell lysate.
Specific Publications For Anti-PDCD4 Rabbit Monoclonal Antibody (M01105)
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5 Customer Q&As for Anti-PDCD4 Rabbit Monoclonal Antibody
Question
Will M01105 anti-PDCD4 Monoclonal antibody work on parafin embedded sections? If so, which fixation method do you recommend we use (PFA, paraformaldehyde, other)?
H. Williams
Verified customer
Asked: 2020-03-27
Answer
You can see on the product datasheet, M01105 anti-PDCD4 Monoclonal antibody as been tested on IF. It is best to use PFA for fixation because it has better tissue penetration ability. PFA needs to be prepared fresh before use. Long term stored PFA turns into formalin, as the PFA molecules congregate and become formalin.
Boster Scientific Support
Answered: 2020-03-27
Question
What sample was used in your WB analysis of M01105?
Verified customer
Asked: 2019-12-18
Answer
For the Anti-PDCD4 Rabbit Monoclonal Antibody (M01105), western blot analysis of PDCD4 expression in HeLa cell lysate.
Boster Scientific Support
Answered: 2019-12-20
Question
I was wanting to use your anti-PDCD4 Monoclonal antibody for IF for mouse body of pancreas on frozen tissues, but I want to know if it has been tested for this particular application. Has this antibody been tested and is this antibody a good choice for mouse body of pancreas identification?
Verified Customer
Verified customer
Asked: 2019-10-14
Answer
As indicated on the product datasheet, M01105 anti-PDCD4 Monoclonal antibody has been validated for IF on human, mouse tissues. We have an innovator award program that if you test this antibody and show it works in mouse body of pancreas in IHC-frozen, you can get your next antibody for free.
Boster Scientific Support
Answered: 2019-10-14
Question
Here is the WB image, lot number and protocol we used for body of pancreas using anti-PDCD4 Monoclonal antibody M01105. Please let me know if you require anything else.
D. Wu
Verified customer
Asked: 2019-02-15
Answer
Thank you very much for the data. Our lab team are working to resolve this as quickly as possible, and we appreciate your patience and understanding! You have provided everything we needed. Please let me know if there is anything you need in the meantime.
Boster Scientific Support
Answered: 2019-02-15
Question
Does anti-PDCD4 Monoclonal antibody M01105 work for IF with body of pancreas?
A. Brown
Verified customer
Asked: 2019-01-28
Answer
According to the expression profile of body of pancreas, PDCD4 is highly expressed in body of pancreas. So, it is likely that anti-PDCD4 Monoclonal antibody M01105 will work for IF with body of pancreas.
Boster Scientific Support
Answered: 2019-01-28
