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Product Info Summary
|Reactive Species:||Human, Mouse|
|Application:||Flow Cytometry, IF, IHC, ICC, WB|
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Anti-PDCD4 Rabbit Monoclonal Antibody
Boster Bio Anti-PDCD4 Rabbit Monoclonal Antibody catalog # M01105. Tested in Flow Cytometry, IF, IHC, ICC, WB applications. This antibody reacts with Human, Mouse.
Storage & Handling
Store at -20°C for one year. For short term storage and frequent use, store at 4°C for up to one month. Avoid repeated freeze-thaw cycles.
Cite This Product
Anti-PDCD4 Rabbit Monoclonal Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # M01105)
Rabbit IgG in phosphate buffered saline, pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol, 0.4-0.5mg/ml BSA.
A synthesized peptide derived from human PDCD4
*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.
M01105 is reactive to PDCD4 in Human, Mouse
M01105 is guaranteed for Flow Cytometry, IF, IHC, ICC, WB Boster Guarantee
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence and ELISA with known positive and negative samples to ensure specificity and high affinity.
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Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. Actual working concentration varies and should be decided by the user.
Validation Images & Assay Conditions
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Figure 1. Western blot analysis of PDCD4 expression in HeLa cell lysate using anti-PDCD4 antibody (M01105).
Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions.
After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-PDCD4 antigen affinity purified polyclonal antibody (Catalog # M01105) at 0.5 ug/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-Rabbit IgG IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # SA1022) with Tanon 5200 system. A specific band was detected for PDCD4.
Gene/Protein Information For PDCD4 (Source: Uniprot.Org, NCBI)
Programmed cell death protein 4
H731; H731nuclear antigen H731; MGC33046; MGC33047; Neoplastic transformation inhibitor protein; Nuclear antigen H731-like; PDCD4; programmed cell death 4 (neoplastic transformation inhibitor); programmed cell death protein 4; Protein 197/15a PDCD4 H731 programmed cell death 4 programmed cell death protein 4|neoplastic transformation inhibitor protein|nuclear antigen H731|programmed cell death 4 (neoplastic transformation inhibitor)|protein 197/15a*if product is indicated to react with multiple species, protein info is based on the gene entry specified above in "species".
For more info on PDCD4, check out the PDCD4 Infographic
We have 30,000+ of these available, one for each gene! check them out.
In this infographic you will see the following information for PDCD4: database IDs, super-family, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact us [email protected]
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