Anti-PDCD4 Rabbit Monoclonal Antibody

Pdcd4 antibody

Boster Bio Anti-PDCD4 Rabbit Monoclonal Antibody catalog # M01105. Tested in WB, IHC, ICC/IF, Flow Cytometry applications. This antibody reacts with Human, Mouse.

Product Info Summary

SKU: M01105
Size: 100 μl
Reactive Species: Human, Mouse
Host: Rabbit
Application: Flow Cytometry, IF, IHC, ICC, WB

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Product Name

Anti-PDCD4 Rabbit Monoclonal Antibody

View all Pdcd4 Antibodies

SKU/Catalog Number

M01105

Size

100 μl

Form

Liquid

Description

Boster Bio Anti-PDCD4 Rabbit Monoclonal Antibody catalog # M01105. Tested in WB, IHC, ICC/IF, Flow Cytometry applications. This antibody reacts with Human, Mouse.

Storage & Handling

Store at -20°C for one year. For short term storage and frequent use, store at 4°C for up to one month. Avoid repeated freeze-thaw cycles.

Cite This Product

Anti-PDCD4 Rabbit Monoclonal Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # M01105)

Host

Rabbit

Contents

Rabbit IgG in phosphate buffered saline, pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol, 0.4-0.5mg/ml BSA.

Clonality

Monoclonal

Clone Number

ACAE-16

Isotype

Rabbit IgG

Immunogen

A synthesized peptide derived from human PDCD4

*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.

Cross-reactivity

Detects ~20kDa. Does not cross-react with αB-crystallin, βL-crystallin, ΒH- crystallin, γ-crystallin, HSP25, HSP27 or HSP47 proteins.

Reactive Species

M01105 is reactive to Pdcd4 in Human, Mouse

Applications

M01105 is guaranteed for Flow Cytometry, IF, IHC, ICC, WB Boster Guarantee

Observed Molecular Weight

80-100 kDa

Calculated molecular weight

51.702kDa

Background of Pdcd4

Activated by icilin, eucalyptol, menthol, cold and modulation of intracellular pH. Involved in menthol sensation. Permeable for monovalent cations sodium, potassium, and cesium and divalent cation calcium.

Antibody Validation

Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.

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Reconsitution

Restore with deionized water (or equivalent) for reconstitution volume of 1.0 mL

Assay Dilutions Recommendation

The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.

WB 1:500-1:2000
IHC 1:100-1:500
ICC/IF 1:50-1:200
FC 1:50

Validation Images & Assay Conditions

Gene/Protein Information For Pdcd4 (Source: Uniprot.org, NCBI)

Gene Name

Pdcd4

Full Name

Programmed cell death protein 4

Weight

51.702kDa

Superfamily

PDCD4 family

Alternative Names

H731; H731nuclear antigen H731; MGC33046; MGC33047; Neoplastic transformation inhibitor protein; Nuclear antigen H731-like; PDCD4; programmed cell death 4 (neoplastic transformation inhibitor); programmed cell death protein 4; Protein 197/15a Pdcd4|D19Ucl, D19Ucla1, MA-, Ma3, T, Tis|programmed cell death 4|programmed cell death protein 4|protein MA-3|topoisomerase-inhibitor suppressed protein

*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".

For more info on Pdcd4, check out the Pdcd4 Infographic

Pdcd4 infographic

We have 30,000+ of these available, one for each gene! Check them out.

In this infographic, you will see the following information for Pdcd4: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].

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5 Customer Q&As for Anti-PDCD4 Rabbit Monoclonal Antibody

Question

Will M01105 anti-PDCD4 Monoclonal antibody work on parafin embedded sections? If so, which fixation method do you recommend we use (PFA, paraformaldehyde, other)?

H. Williams

Verified customer

Asked: 2020-03-27

Answer

You can see on the product datasheet, M01105 anti-PDCD4 Monoclonal antibody as been tested on IF. It is best to use PFA for fixation because it has better tissue penetration ability. PFA needs to be prepared fresh before use. Long term stored PFA turns into formalin, as the PFA molecules congregate and become formalin.

Boster Scientific Support

Answered: 2020-03-27

Question

What sample was used in your WB analysis of M01105?

Verified customer

Asked: 2019-12-18

Answer

For the Anti-PDCD4 Rabbit Monoclonal Antibody (M01105), western blot analysis of PDCD4 expression in HeLa cell lysate.

Boster Scientific Support

Answered: 2019-12-20

Question

I was wanting to use your anti-PDCD4 Monoclonal antibody for IF for mouse body of pancreas on frozen tissues, but I want to know if it has been tested for this particular application. Has this antibody been tested and is this antibody a good choice for mouse body of pancreas identification?

Verified Customer

Verified customer

Asked: 2019-10-14

Answer

As indicated on the product datasheet, M01105 anti-PDCD4 Monoclonal antibody has been validated for IF on human, mouse tissues. We have an innovator award program that if you test this antibody and show it works in mouse body of pancreas in IHC-frozen, you can get your next antibody for free.

Boster Scientific Support

Answered: 2019-10-14

Question

Here is the WB image, lot number and protocol we used for body of pancreas using anti-PDCD4 Monoclonal antibody M01105. Please let me know if you require anything else.

D. Wu

Verified customer

Asked: 2019-02-15

Answer

Thank you very much for the data. Our lab team are working to resolve this as quickly as possible, and we appreciate your patience and understanding! You have provided everything we needed. Please let me know if there is anything you need in the meantime.

Boster Scientific Support

Answered: 2019-02-15

Question

Does anti-PDCD4 Monoclonal antibody M01105 work for IF with body of pancreas?

A. Brown

Verified customer

Asked: 2019-01-28

Answer

According to the expression profile of body of pancreas, PDCD4 is highly expressed in body of pancreas. So, it is likely that anti-PDCD4 Monoclonal antibody M01105 will work for IF with body of pancreas.

Boster Scientific Support

Answered: 2019-01-28

Order DetailsPrice
M01105

100μL

$370
M01105-30ul

30μL sample

$99
M01105-carrier-free

Carrier Free

$370

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*Sample sizes are prepared on demand and will take extra lead time. (cannot be conjugated)
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