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- Table of Contents
PDGFRB antibody (A00096-1) performs well in ELISA, Flow Cytometry, WB and shows reactivity with Human, Mouse, Rat.
- Validated for inter-lot consistency, specificity, and reproducibility
- Committed to quality since 1993
- Over 6 Publications
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Flow Cytometry analysis of SH-SY5Y cells using anti-PDGFRB antibody (A00096-1).
Overlay histogram showing SH-SY5Y cells stained with A00096-1 (Blue line). The cells were fixed with 4% paraformaldehyde and blocked with 10% normal goat serum. And then incubated with rabbit anti-PDGFRB Antibody (A00096-1, 1 μg/1x106 cells) for 30 min at 20°C. DyLight®488 conjugated goat anti-rabbit IgG (BA1127, 5-10 μg/1x106 cells) was used as secondary antibody for 30 minutes at 20°C. Isotype control antibody (Green line) was rabbit IgG (1 μg/1x106) used under the same conditions. Unlabelled sample without incubation with primary antibody and secondary antibody (Red line) was used as a blank control.
Overlay histogram showing SH-SY5Y cells stained with A00096-1 (Blue line). The cells were fixed with 4% paraformaldehyde and blocked with 10% normal goat serum. And then incubated with rabbit anti-PDGFRB Antibody (A00096-1, 1 μg/1x106 cells) for 30 min at 20°C. DyLight®488 conjugated goat anti-rabbit IgG (BA1127, 5-10 μg/1x106 cells) was used as secondary antibody for 30 minutes at 20°C. Isotype control antibody (Green line) was rabbit IgG (1 μg/1x106) used under the same conditions. Unlabelled sample without incubation with primary antibody and secondary antibody (Red line) was used as a blank control.
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Anti-PDGF Receptor beta/PDGFRB Antibody Picoband®
PDGFRB antibody
| Reactive Species | Host | Application |
|---|---|---|
| Human, Mouse, Rat | Rabbit | ELISA, Flow Cytometry, WB |
Tested Applications
PDGFRB antibody was positively detected by WB in:
human SH-SY5Y whole cell, human U2OS whole cell, rat ovary tissue, mouse lung tissue
Suggested blocking solution with 5% non-fat milk or BSA; (*)Recommended protein loading: 20-40 ug per lane
PDGFRB antibody was positively detected by FC in:
SH-SY5Y cell
Recommend Dilution
Application
Dilution
Species
Western blot
0.1-0.5μg/ml
Human, Mouse, Rat
Flow Cytometry(Fixed)
1-3 μg/1x106 cells
Human
ELISA
0.1-0.5μg/ml
* It is recommended to optimize the amount of this reagent in each experimental system to achieve the best results.
Note: For flow cytometry (FCM), suitable for fixed cells only. Not suitable for FACS or surface flow cytometry using live cells.
Reactive Data
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WB
ELISA
Human
Tested
Recommend
Rat
Tested
Recommend
Mouse
Tested
Recommend
Bovine
Predicted
Predicted
Sheep
Predicted
Predicted
Monkey
Predicted
Predicted
Rabbit
Predicted
Predicted
Dog
Predicted
Predicted
Product Information
Product Name
Anti-PDGF Receptor beta/PDGFRB Antibody Picoband®
View all PDGFRB Antibodies
View all PDGFRB Antibodies
Form
Lyophilized
Uniprot
Gene ID
Storage & Handling
Store at -20˚C for one year from date of receipt. After reconstitution, at 4˚C for one month. It can also be aliquotted and stored frozen at -20˚C for six months. Avoid repeated freeze-thaw cycles.
Host
Rabbit
Contents
Each vial contains 4 mg Trehalose, 0.9 mg NaCl and 0.2 mg Na2HPO4.
Clonality
Polyclonal
Isotype
Rabbit IgG
Immunogen
E. coli-derived human PDGFRB recombinant protein (Position: E911-L992).
Cross-Reactivity
No cross-reactivity with other proteins.
Reconstitution
Add 0.2ml of distilled water will yield a concentration of 500ug/ml.
Observed Molecular Weight
190 kDa
Calculated Molecular Weight
124.0 kDa
Background of PDGFRB
Beta-type platelet-derived growth factor receptor is a protein that in humans is encoded by the PDGFRB gene. The protein encoded by this gene is a cell surface tyrosine kinase receptor for members of the platelet-derived growth factor family. These growth factors are mitogens for cells of mesenchymal origin. The identity of the growth factor bound to a receptor monomer determines whether the functional receptor is a homodimer (PDGFB or PDGFD) or a heterodimer (PDGFA and PDGFB). This gene is essential for normal development of the cardiovascular system and aids in rearrangement of the actin cytoskeleton. This gene is flanked on chromosome 5 by the genes for granulocyte-macrophage colony-stimulating factor and macrophage-colony stimulating factor receptor; all three genes may be implicated in the 5-q syndrome. A translocation between chromosomes 5 and 12, that fuses this gene to that of the ETV6 gene, results in chronic myeloproliferative disorder with eosinophilia.
Cite This Product
Anti-PDGF Receptor beta/PDGFRB Antibody Picoband® (Boster Biological Technology, Pleasanton CA, USA, Catalog # A00096-1)
Antibody Homology Across Species
The results generated by this homology analysis tool for commonly used animal species are shown below. For unlisted species, please refer to the homology analysis between the immunogen sequence and the corresponding species.
A homology of ≥90% is recommended. Generally, higher homology indicates a greater potential for cross-species reactivity. However, protein expression levels may vary among different species, tissues, and cell types. Therefore, the homology analysis results are for reference only and do not guarantee antibody cross-reactivity or experimental performance.
Human
100%
High Homology
Cow
100%
High Homology
Sheep
100%
High Homology
Monkey
100%
High Homology
Rabbit
99%
High Homology
Dog
99%
High Homology
Rat
98%
High Homology
Mouse
96%
High Homology
Zebrafish
60%
Low homology
Drosophila
34%
Low homology
Homology ≥ 90%High Homology
70% ≤ Homology < 90%Moderate Homology
Homology < 70%Low homology
Protocols
Western Blot protocol for PDGFRB antibody
Flow Cytometry (FC) protocol for PDGFRB antibody
Publications
Application:
Species:
Electroacupuncture Prevents Against AD‐Like Phenotypes in APP/PS1 Mice: Investigation of the Mechanisms From Cerebral Microangiopathy
Published: 2025-12-09
Journal: CNS Neuroscience & Therapeutics
Application: WB
Impact Factor: 5.00
Species: Mouse
PMID: 41367129
Pan-cancer analysis of PDGFRB: Laying the foundation for the development of targeted immunotherapy drugs
Published: 2025-03-21
Journal: MEDICINE
Application: IHC
Impact Factor: 1.40
Species: Human
PMID: 40128057
GLI2 induces PDGFRB expression and modulates cancer stem cell properties of gastric cancer
Published: 2017-10-01
Journal: European Review for Medical and Pharmacological Sciences
Application: WB
Impact Factor: 1.78
Species: Human
PMID: 28975979
Protective effects of glutamine in a rat model of endotoxemia
Published: 2012-07-26
Journal: Molecular Medicine Reports
Application: WB,IHC
Impact Factor: 0.42
Species: Rat
PMID: 22842774
RNA interference targeting the platelet-derived growth factor receptor β subunit ameliorates experimental hepatic fibrosis in rats
Published: 2008-10-10
Journal: LIVER INTERNATIONAL
Application: IHC
Impact Factor: 2.56
Species: Rat
PMID: 18466260
Effects of isoliensinine on angiotensin II-induced proliferation of porcine coronary arterial smooth muscle cells
Published: 2006-09-04
Journal: JOURNAL OF ASIAN NATURAL PRODUCTS RESEARCH
Impact Factor: 1.07
Species: Pig
PMID: 16864426
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Product Promise
We promise Anti-PDGF Receptor beta/PDGFRB Antibody Picoband® performs excellently in relevant applications for ELISA, Flow Cytometry, WB. If it doesn't perform as promised, we will refund the product. Learn more
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A00096-1
Buy one primary antibody get one 0.5ml HRP or Biotin secondary antibody for free.
*Sample sizes are prepared on demand and will take extra lead time. (cannot be conjugated)
$370.00
Troubleshooting
Boster Guarantee
Guaranteed product quality
We promise all of our products perform as described in datasheets.
This product is for Research Use Only. Not for use in diagnostic procedures.
Update date:
Oct 22, 2026