Click image to see more details
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Lane 1: K562 whole cell lysate
Lane 2: CEM whole cell lysate
Secondary
Goat Anti-mouse IgG, (H+L),Peroxidase conjugated at 1/10000 dilution.
Predicted band size : 46469 Da
Blocking/Dilution buffer: 5% NFDM/TBST."" alt="m02132 anti pdk4 antibody wb testing 3">
Product Info Summary
| SKU: | M02132 |
|---|---|
| Size: | 80 µl |
| Reactive Species: | Human, Mouse |
| Host: | Mouse |
| Application: | WB |
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Product info
Product Name
Anti-PDK4 Antibody
SKU/Catalog Number
M02132
Size
80 µl
Description
Boster Bio Anti-PDK4 Antibody (Catalog # M02132). Tested in WB application(s). This antibody reacts with Human, Mouse.
Storage & Handling
Maintain refrigerated at 2-8°C for up to 2 weeks. For long-term storage, store at -20°C in small aliquots to prevent freeze-thaw cycles.
Cite This Product
Anti-PDK4 Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # M02132)
Host
Mouse
Contents
Purified monoclonal antibody supplied in PBS with 0.09% (W/V) sodium azide.
Clonality
Monoclonal
Clone Number
345CT19.6.2
Isotype
IgG1
Immunogen
Purified His-tagged PDK4 protein (Fragment) was used to produced this monoclonal antibody.
Reactive Species
M02132 is reactive to PDK4 in Human, Mouse
Calculated molecular weight
46.5 kDa
Background of PDK4
This gene is a member of the PDK/BCKDK protein kinase family and encodes a mitochondrial protein with a histidine kinase domain. This protein is located in the matrix of the mitrochondria and inhibits the pyruvate dehydrogenase complex by phosphorylating one of its subunits, thereby contributing to the regulation of glucose metabolism. Expression of this gene is regulated by glucocorticoids, retinoic acid and insulin.
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
M02132 is guaranteed for WB Boster Guarantee
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
WB: 1:250
Validation Images & Assay Conditions
Click image to see more details
Anti-PDK4 Antibody at 1:1000 dilution + human heart lysate
Lysates/proteins at 20 µg per lane.
Secondary
Goat Anti-mouse IgG, (H+L), Peroxidase conjugated at 1/10000 dilution.
Predicted band size : 46 kDa
Blocking/Dilution buffer: 5% NFDM/TBST.
Click image to see more details
All lanes : Anti-PDK4 Antibody at 1:500-1:1000 dilution
Lane 1: K562 whole cell lysate
Lane 2: Human heart lysate
Lane 3: Mouse heart lysate
Lysates/proteins at 20 µg per lane.
Secondary
Goat Anti-mouse IgG, (H+L), Peroxidase conjugated at 1/10000 dilution.
Predicted band size : 46 kDa
Blocking/Dilution buffer: 5% NFDM/TBST.
Click image to see more details
"All lanes : Anti-PDK4 Antibody at 1:250 dilution
Lane 1: K562 whole cell lysate
Lane 2: CEM whole cell lysate
Secondary
Goat Anti-mouse IgG, (H+L),Peroxidase conjugated at 1/10000 dilution.
Predicted band size : 46469 Da
Blocking/Dilution buffer: 5% NFDM/TBST."
Specific Publications For Anti-PDK4 Antibody (M02132)
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