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PDZK1 antibody (A03176-1) performs well in ELISA, Flow Cytometry, IHC, WB and shows reactivity with Human, Mouse, Rat.
- Validated for inter-lot consistency, specificity, and reproducibility
- Committed to quality since 1993
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Flow Cytometry analysis of RM-1 cells using anti-PDZK1 antibody (A03176-1).
Overlay histogram showing RM-1 cells stained with A03176-1 (Blue line). The cells were fixed with 4% paraformaldehyde and blocked with 10% normal goat serum. And then incubated with rabbit anti-PDZK1 Antibody (A03176-1, 1 μg/1x106 cells) for 30 min at 20°C. DyLight®488 conjugated goat anti-rabbit IgG (BA1127, 5-10 μg/1x106 cells) was used as secondary antibody for 30 minutes at 20°C. Isotype control antibody (Green line) was rabbit IgG (1 μg/1x106) used under the same conditions. Unlabelled sample without incubation with primary antibody and secondary antibody (Red line) was used as a blank control.
Overlay histogram showing RM-1 cells stained with A03176-1 (Blue line). The cells were fixed with 4% paraformaldehyde and blocked with 10% normal goat serum. And then incubated with rabbit anti-PDZK1 Antibody (A03176-1, 1 μg/1x106 cells) for 30 min at 20°C. DyLight®488 conjugated goat anti-rabbit IgG (BA1127, 5-10 μg/1x106 cells) was used as secondary antibody for 30 minutes at 20°C. Isotype control antibody (Green line) was rabbit IgG (1 μg/1x106) used under the same conditions. Unlabelled sample without incubation with primary antibody and secondary antibody (Red line) was used as a blank control.
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Anti-PDZK1 Antibody Picoband®
PDZK1 antibody
| Reactive Species | Host | Application |
|---|---|---|
| Human, Mouse, Rat | Rabbit | ELISA, Flow Cytometry, IHC, WB |
Tested Applications
PDZK1 antibody was positively detected by WB in:
human 293T whole cell, human Caco-2 whole cell, human HepG2 whole cell, human MCF-7 whole cell, rat kidney tissue, rat liver tissue, mouse kidney tissue, mouse liver tissue, mouse brain tissue
Suggested blocking solution with 5% non-fat milk or BSA; (*)Recommended protein loading: 20-40 ug per lane
PDZK1 antibody was positively detected by IHC in:
human liver tissue
Use EDTA pH 8.0 for antigen retrieval; (*) citrate buffer pH 6.0 is an alternative.
PDZK1 antibody was positively detected by FC in:
RM-1 cell, CACO-2 cell
Recommend Dilution
Application
Dilution
Species
Western blot
0.25-0.5 μg/ml
Human, Mouse, Rat
Immunohistochemistry (Paraffin-embedded Section)
2-5 μg/ml
Human
Flow Cytometry (Fixed)
1-3 μg/1x106 cells
Human, Mouse
ELISA
0.1-0.5 μg/ml
* It is recommended to optimize the amount of this reagent in each experimental system to achieve the best results.
Note: For flow cytometry (FCM), suitable for fixed cells only. Not suitable for FACS or surface flow cytometry using live cells.
Reactive Data
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WB
IHC
IF
ELISA
Human
Tested
Tested
Recommend
Recommend
Rat
Tested
Recommend
Mouse
Tested
Recommend
Product Information
Product Name
Anti-PDZK1 Antibody Picoband®
View all PDZK1 Antibodies
View all PDZK1 Antibodies
Form
Lyophilized
Uniprot
Gene ID
Storage & Handling
At -20°C for one year from date of receipt.
After reconstitution, at 4°C for one month. It can also be aliquotted and stored frozen at -20°C for six months.
Avoid repeated freezing and thawing.
Host
Rabbit
Contents
Each vial contains 4 mg Trehalose, 0.9 mg NaCl, 0.2 mg Na2HPO4.
Clonality
Polyclonal
Immunogen
E.coli-derived mouse PDZK1 recombinant protein (Position: R8-R502). Mouse PDZK1 shares 78% and 91.4% amino acid (aa) sequence identity with human and rat PDZK1, respectively.
Reconstitution
Adding 0.2 ml of distilled water will yield a concentration of 500 μg/ml.
Observed Molecular Weight
70 kDa
Calculated Molecular Weight
56.5 kDa
Background of PDZK1
Predicted to enable PDZ domain binding activity; protein-membrane adaptor activity; and scavenger receptor binding activity. Involved in positive regulation of protein targeting to membrane; protein localization to plasma membrane; and regulation of monoatomic anion transport. Acts upstream of or within in utero embryonic development. Located in brush border membrane. Is expressed in several structures, including genitourinary system; liver; lung; thymus; and white fat. Orthologous to several human genes including PDZK1 (PDZ domain containing 1).
Cite This Product
Anti-PDZK1 Antibody Picoband® (Boster Biological Technology, Pleasanton CA, USA, Catalog # A03176-1)
Antibody Homology Across Species
The results generated by this homology analysis tool for commonly used animal species are shown below. For unlisted species, please refer to the homology analysis between the immunogen sequence and the corresponding species.
A homology of ≥90% is recommended. Generally, higher homology indicates a greater potential for cross-species reactivity. However, protein expression levels may vary among different species, tissues, and cell types. Therefore, the homology analysis results are for reference only and do not guarantee antibody cross-reactivity or experimental performance.
Mouse
100%
High Homology
Rat
92%
High Homology
Sheep
78%
Moderate Homology
Human
78%
Moderate Homology
Monkey
78%
Moderate Homology
Cow
78%
Moderate Homology
Rabbit
77%
Moderate Homology
Dog
76%
Moderate Homology
Zebrafish
45%
Low homology
Drosophila
9%
Low homology
Homology ≥ 90%High Homology
70% ≤ Homology < 90%Moderate Homology
Homology < 70%Low homology
Protocols
Western Blot protocol for PDZK1 antibody
Immunohistochemistry (IHC) protocol for PDZK1 antibody
Flow Cytometry (FC) protocol for PDZK1 antibody
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Product Promise
We promise Anti-PDZK1 Antibody Picoband® performs excellently in relevant applications for ELISA, Flow Cytometry, IHC, WB. If it doesn't perform as promised, we will refund the product. Learn more
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A03176-1
Buy one primary antibody get one 0.5ml HRP or Biotin secondary antibody for free.
*Sample sizes are prepared on demand and will take extra lead time. (cannot be conjugated)
$370.00
Troubleshooting
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We promise all of our products perform as described in datasheets.
This product is for Research Use Only. Not for use in diagnostic procedures.
Update date:
Oct 5, 2026