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Product Info Summary
| SKU: | M02034 |
|---|---|
| Size: | 100 μl |
| Reactive Species: | Human |
| Host: | Rabbit |
| Application: | Flow Cytometry, WB |
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Product info
Product Name
Anti-PEDF Monoclonal Antibody
SKU/Catalog Number
M02034
BM5733 is an alternative SKU for this antibody, used in previous lots.
Size
100 μl
Form
Liquid
Description
Boster Bio Anti-PEDF Monoclonal Antibody catalog # M02034. Tested in WB, Flow Cytometry applications. This antibody reacts with Human.
Storage & Handling
Store at -20°C for one year. For short term storage and frequent use, store at 4°C for up to one month. Avoid repeated freeze-thaw cycles.
Cite This Product
Anti-PEDF Monoclonal Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # M02034)
Host
Rabbit
Contents
Rabbit IgG in stabilizing components, phosphate buffered saline, pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
*This antibody is supplied in a stabilized formulation.
Compatibility with conjugation reactions depends on the chemistry of the conjugation method used.
For conjugation methods that are not compatible with the stabilizing components present in this formulation, a carrier-free antibody format is required.
Clonality
Monoclonal
Clone Number
AFED-19
Isotype
Rabbit IgG
Immunogen
A synthesized peptide derived from human PEDF Neurotrophic protein; induces extensive neuronal differentiation in retinoblastoma cells. Potent inhibitor of angiogenesis. As it does not undergo the S (stressed) to R (relaxed) conformational transition characteristic of active serpins, it exhibits no serine protease inhibitory activity.
Reactive Species
M02034 is reactive to SERPINF1 in Human
Observed Molecular Weight
50 kDa
Calculated molecular weight
46.3 kDa
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
M02034 is guaranteed for Flow Cytometry, WB Boster Guarantee
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
WB 1:500-2000
FC 1:100
Positive Control
WB: human K562 whole cell, human HepG2 whole cell, human SH-SY5Y whole cell
Validation Images & Assay Conditions
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Western blot analysis of PEDF using anti-PEDF antibody (M02034).
Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions.
Lane 1: human K562 whole cell lysates,
Lane 2: human HepG2 whole cell lysates,
Lane 3: human SH-SY5Y whole cell lysates.
After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-PEDF antigen affinity purified monoclonal antibody (Catalog # M02034) at 1:500 overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:500 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for PEDF at approximately 50 kDa. The expected band size for PEDF is at 46,50 kDa.
Specific Publications For Anti-PEDF Monoclonal Antibody (M02034)
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4 Customer Q&As for Anti-PEDF Monoclonal Antibody
Question
What sample was tested in WB and IHC applications with M02034?
Verified customer
Asked: 2020-01-09
Answer
Western blot analysis of PEDF expression in K562 cell lysate.
Boster Scientific Support
Answered: 2020-01-09
Question
Is there a BSA free version of anti-PEDF Monoclonal antibody M02034 available?
Verified Customer
Verified customer
Asked: 2018-04-06
Answer
I appreciate your recent telephone inquiry. I can confirm that some lots of this anti-PEDF Monoclonal antibody M02034 are BSA free. For now, these lots are available and we can make a BSA free formula for you free of charge. It will take 3 extra days to prepare. If you require this antibody BSA free again in future, please do not hesitate to contact me and I will be pleased to check which lots we have in stock that are BSA free.
Boster Scientific Support
Answered: 2018-04-06
Question
Will M02034 anti-PEDF Monoclonal antibody work on parafin embedded sections? If so, which fixation method do you recommend we use (PFA, paraformaldehyde, other)?
Verified Customer
Verified customer
Asked: 2018-02-06
Answer
As indicated on the product datasheet, M02034 anti-PEDF Monoclonal antibody as been validated on WB. It is best to use PFA for fixation because it has better tissue penetration ability. PFA needs to be prepared fresh before use. Long term stored PFA turns into formalin, as the PFA molecules congregate and become formalin.
Boster Scientific Support
Answered: 2018-02-06
Question
We are currently using anti-PEDF Monoclonal antibody M02034 for human tissue, and we are happy with the WB results. The species of reactivity given in the datasheet says human. Is it likely that the antibody can work on monkey tissues as well?
N. Parker
Verified customer
Asked: 2014-04-04
Answer
The anti-PEDF Monoclonal antibody (M02034) has not been tested for cross reactivity specifically with monkey tissues, but there is a good chance of cross reactivity. We have an innovator award program that if you test this antibody and show it works in monkey you can get your next antibody for free. Please contact me if I can help you with anything.
Boster Scientific Support
Answered: 2014-04-04
