Anti-Perilipin 3/PLIN3 Antibody Picoband™

Product Name

Anti-Perilipin 3/PLIN3 Antibody Picoband™

View all Perilipin-3/TIP47 Antibodies

SKU/Catalog Number

PB10013

Size

100 μg/vial

Form

Lyophilized

Description

Boster Bio Anti-Perilipin 3/PLIN3 Antibody Picoband™ catalog # PB10013. Tested in IHC, WB applications. This antibody reacts with Human, Mouse, Rat.

Storage & Handling

Store at -20˚C for one year from date of receipt. After reconstitution, at 4˚C for one month. It can also be aliquotted and stored frozen at -20˚C for six months. Avoid repeated freeze-thaw cycles.

Cite This Product

Anti-Perilipin 3/PLIN3 Antibody Picoband™ (Boster Biological Technology, Pleasanton CA, USA, Catalog # PB10013)

Host

Rabbit

Contents

Each vial contains 5mg BSA, 0.9mg NaCl, 0.2mg Na2HPO4, 0.05mg NaN3.

Clonality

Polyclonal

Isotype

Rabbit IgG

Immunogen

A synthetic peptide corresponding to a sequence at the C-terminus of human Perilipin 3, different from the related mouse sequence by fourteen amino acids.

*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.

Cross-reactivity

No cross-reactivity with other proteins

Reactive Species

PB10013 is reactive to PLIN3 in Human, Mouse, Rat

Applications

PB10013 is guaranteed for IHC, WB Boster Guarantee

Observed Molecular Weight

47 kDa

Calculated molecular weight

47.075kDa

Background of Perilipin-3/TIP47

Mannose-6-phosphate receptor binding protein 1 (M6PRBP1), also known as Perilipin 3 (PLN3) or TIP47, is a protein which in humans is encoded by the M6PRBP1 gene. Mannose 6-phophate receptors (MPRs) deliver lysosomal hydrolase from the Golgi to endosomes and then return to the Golgi complex. The protein encoded by this gene interacts with the cytoplasmic domains of both cation-independent and cation-dependent MPRs, and is required for endosome-to-Golgi transport. This protein also binds directly to the GTPase RAB9 (RAB9A), a member of the RAS oncogene family. The interaction with RAB9 has been shown to increase the affinity of this protein for its cargo. Multiple transcript variants encoding different isoforms have been found for this gene.

Antibody Validation

Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.

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Reconsitution

Add 0.2ml of distilled water will yield a concentration of 500ug/ml.

Assay Dilutions Recommendation

The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.

Immunohistochemistry (Paraffin-embedded Section), 0.5-1μg/ml, Human, By Heat
Western blot, 0.1-0.5μg/ml, Human, Mouse, Rat

Validation Images & Assay Conditions

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Figure 2. IHC analysis of Perilipin 3 using anti-Perilipin 3 antibody (PB10013).
Perilipin 3 was detected in a paraffin-embedded section of human lung cancer tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1 μg/ml rabbit anti-Perilipin 3 Antibody (PB10013) overnight at 4°C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC) (Catalog # SA1022) with DAB as the chromogen.

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Figure 1. Western blot analysis of Perilipin 3 using anti-Perilipin 3 antibody (PB10013).
Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions.
Lane 1: rat liver tissue lysates,
Lane 2: mouse kidney tissue lysates,
Lane 3: HELA whole cell lysates.
After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-Perilipin 3 antigen affinity purified polyclonal antibody (Catalog # PB10013) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for Perilipin 3 at approximately 47 kDa. The expected band size for Perilipin 3 is at 47 kDa.

Gene/Protein Information For PLIN3 (Source: Uniprot.org, NCBI)

Alternative Names

Cargo selection protein TIP47; M6PRBP1; M6PRBP1MGC2012; Mannose-6-phosphate receptor-binding protein 1; perilipin 3,47 kDa MPR-binding protein; Perilipin3; Perilipin-3; Placental protein 17,47 kDa mannose 6-phosphate receptor-binding protein; PLIN3; PP17; PP17MGC11117; tail-interacting protein, 47 kD; TIP47; TIP47mannose-6-phosphate receptor binding protein 1 PLIN3 M6PRBP1, PP17, TIP47 perilipin 3 perilipin-3|47 kDa MPR-binding protein|cargo selection protein TIP47|mannose-6-phosphate receptor-binding protein 1|placental protein 17|tail-interacting protein, 47 kD|testicular tissue protein Li 114

For more info on PLIN3, check out the PLIN3 Infographic

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In this infographic, you will see the following information for PLIN3: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].

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