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CAV1 antibody (M00179-1) performs well in IF, IHC, IHC-F, WB and shows reactivity with Human.
- Validated for inter-lot consistency, specificity, and reproducibility
- Committed to quality since 1993
- Over 2 Publications
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IF analysis of Caveolin-1/CAV1 using anti-Caveolin-1/CAV1 antibody (M00179-1).
Caveolin-1/CAV1 was detected in paraffin-embedded section of human colorectal cancer tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2μg/mL mouse anti-Caveolin-1/CAV1 Antibody (M00179-1) overnight at 4°C. DyLight®488 Conjugated Goat Anti-Mouse IgG (BA1126) was used as secondary antibody at 1:100 dilution and incubated for 30 minutes at 37°C. Visualize using a fluorescence microscope and filter sets appropriate for the label used.
Caveolin-1/CAV1 was detected in paraffin-embedded section of human colorectal cancer tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2μg/mL mouse anti-Caveolin-1/CAV1 Antibody (M00179-1) overnight at 4°C. DyLight®488 Conjugated Goat Anti-Mouse IgG (BA1126) was used as secondary antibody at 1:100 dilution and incubated for 30 minutes at 37°C. Visualize using a fluorescence microscope and filter sets appropriate for the label used.
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Anti-Caveolin-1/CAV1 Antibody Picoband® (monoclonal, 12C7)
CAV1 antibody
| Reactive Species | Host | Application |
|---|---|---|
| Human | Mouse | IF, IHC, IHC-F, WB |
Tested Applications
CAV1 antibody was positively detected by WB in:
human Hela whole cell,, human A549 whole cell,, human placenta tissue,, human U-87 whole cell,, human PC-3 whole cell,, human U20S whole cell
Suggested blocking solution with 5% non-fat milk or BSA; (*)Recommended protein loading: 20-40 ug per lane
CAV1 antibody was positively detected by IHC in:
human intestinal cancer tissue, human lung cancer tissue, human melanoma tissue, human intestinal cancer tissue
Use TE buffer pH 9.0 for antigen retrieval; (*) citrate buffer pH 6.0 is an alternative.
CAV1 antibody was positively detected by IHC-F in:
human placenta tissue
CAV1 antibody was positively detected by IF in:
human colorectal cancer tissue, human lung cancer tissue, human lung cancer tissue
Recommend Dilution
Application
Dilution
Species
Western blot
0.1-0.5μg/ml
Immunohistochemistry (Paraffin-embedded Section)
0.5-1μg/ml
Immunohistochemistry (Frozen Section)
0.5-1μg/ml
Immunofluorescence
2μg/ml
* It is recommended to optimize the amount of this reagent in each experimental system to achieve the best results.
Reactive Data
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WB
IHC
IF
Human
Tested
Tested
Tested
Bovine
Predicted
Predicted
Predicted
Sheep
Predicted
Predicted
Predicted
Dog
Predicted
Predicted
Predicted
Monkey
Predicted
Predicted
Predicted
Rabbit
Predicted
Predicted
Predicted
Mouse
Predicted
Predicted
Predicted
Rat
Predicted
Predicted
Predicted
Product Information
Product Name
Anti-Caveolin-1/CAV1 Antibody Picoband® (monoclonal, 12C7)
View all CAV1 Antibodies
View all CAV1 Antibodies
Form
Lyophilized
Uniprot
Gene ID
Storage & Handling
Store at -20˚C for one year from date of receipt. After reconstitution, at 4˚C for one month. It can also be aliquotted and stored frozen at -20˚C for six months. Avoid repeated freeze-thaw cycles.
Host
Mouse
Contents
Each vial contains 4mg Trehalose, 0.9mg NaCl, 0.2mg Na2HPO4, 0.05mg NaN3.
Clonality
Monoclonal
Clone Number
12C7
Isotype
Mouse IgG2a
Immunogen
E.coli-derived human Caveolin-1 recombinant protein (Position: G4-I178). Human Caveolin-1 shares 95% and 94% amino acid (aa) sequence identity with mouse and rat Caveolin-1, respectively.
Cross-Reactivity
No cross-reactivity with other proteins.
Reconstitution
Add 0.2ml of distilled water will yield a concentration of 500μg/ml.
Observed Molecular Weight
22 kDa
Calculated Molecular Weight
20.5 kDa
Background of CAV1
CAV1 ( Caveolin-1) is a protein that in humans is encoded by the CAV1 gene. The CAV1 gene is mapped to 7q31.2. The scaffolding protein encoded by this gene is the main component of the caveolae plasma membranes found in most cell types. The protein links integrin subunits to the tyrosine kinase FYN, an initiating step in coupling integrins to the Ras-ERK pathway and promoting cell cycle progression. The gene is a tumor suppressor gene candidate and a negative regulator of the Ras-p42/44 MAP kinase cascade. CAV1 and CAV2 are located next to each other on chromosome 7 and express colocalizing proteins that form a stable hetero-oligomeric complex. By using alternative initiation codons in the same reading frame, two isoforms (alpha and beta) are encoded by a single transcript from this gene.
Cite This Product
Anti-Caveolin-1/CAV1 Antibody Picoband® (monoclonal, 12C7) (Boster Biological Technology, Pleasanton CA, USA, Catalog # M00179-1)
Antibody Homology Across Species
The results generated by this homology analysis tool for commonly used animal species are shown below. For unlisted species, please refer to the homology analysis between the immunogen sequence and the corresponding species.
A homology of ≥90% is recommended. Generally, higher homology indicates a greater potential for cross-species reactivity. However, protein expression levels may vary among different species, tissues, and cell types. Therefore, the homology analysis results are for reference only and do not guarantee antibody cross-reactivity or experimental performance.
Human
100%
High Homology
Cow
96%
High Homology
Sheep
96%
High Homology
Dog
96%
High Homology
Monkey
96%
High Homology
Rabbit
95%
High Homology
Mouse
95%
High Homology
Rat
95%
High Homology
Zebrafish
75%
Moderate Homology
Homology ≥ 90%High Homology
70% ≤ Homology < 90%Moderate Homology
Homology < 70%Low homology
Protocols
Western Blot protocol for CAV1 antibody
Immunohistochemistry (IHC) protocol for CAV1 antibody
Immunofluorescence (IF) protocol for CAV1 antibody
Publications
Application:
Species:
Browning effect of LncRNA00602 in Ad36-induced differentiated adipocytes
Published: 2025-08-22
Journal: MEDICINE
Application: WB
Impact Factor: 1.40
Species: Human
PMID: 40859510
Mechanistic Analysis of Taxol-induced Multidrug Resistance in an Ovarian Cancer Cell Line
Published: 2013-09-30
Journal: Asian Pacific Journal of Cancer Prevention
Application: WB
Impact Factor: 1.27
Species: Human
PMID: 24175763
Review
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Product Promise
We promise Anti-Caveolin-1/CAV1 Antibody Picoband® (monoclonal, 12C7) performs excellently in relevant applications for IF, IHC, IHC-F, WB. If it doesn't perform as promised, we will refund the product. Learn more
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M00179-1
Buy one primary antibody get one 0.5ml HRP or Biotin secondary antibody for free.
*Sample sizes are prepared on demand and will take extra lead time. (cannot be conjugated)
$370.00
Troubleshooting
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We promise all of our products perform as described in datasheets.
This product is for Research Use Only. Not for use in diagnostic procedures.
Update date:
Oct 11, 2026