Anti-PERK/EIF2AK3 Antibody

Boster Bio Anti-PERK/EIF2AK3 Antibody catalog # PA2026. Tested in ICC, WB applications. This antibody reacts with Human. Cited in 1 publication(s).

Product Info Summary

SKU: PA2026
Size: 100μg/vial
Reactive Species: Human
Host: Rabbit
Application: ICC, WB

Product Name

Anti-PERK/EIF2AK3 Antibody

See all PERK products

SKU/Catalog Number

PA2026

Size

100μg/vial

Form

Lyophilized

Description

Boster Bio Anti-PERK/EIF2AK3 Antibody catalog # PA2026. Tested in ICC, WB applications. This antibody reacts with Human.

Storage & Handling

Store at -20˚C for one year from date of receipt. After reconstitution, at 4˚C for one month. It can also be aliquotted and stored frozen at -20˚C for six months. Avoid repeated freeze-thaw cycles.

Cite This Product

Anti-PERK/EIF2AK3 Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # PA2026)

Host

Rabbit

Contents

Each vial contains 5mg BSA, 0.9mg NaCl, 0.2mg Na2HPO4, 0.05mg Thimerosal, 0.05mg NaN3.

Clonality

Polyclonal

Immunogen

A synthetic peptide corresponding to a sequence at the N-terminus of human PERK (163-176aa QWDQDRESMETVPF).

Cross Reactivity

No cross reactivity with other proteins

Reactive Species

PA2026 is reactive to EIF2AK3 in Human

Applications

PA2026 is guaranteed for ICC, WB Boster Guarantee

*Blocking peptide can be purchased at $150. Contact us for more information.

Antibody Validation

Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence and ELISA with known positive and negative samples to ensure specificity and high affinity.

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Innovating Scientists Reward

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Reconsitution

Add 0.2ml of distilled water will yield a concentration of 500ug/ml.

Assay Dilutions Recommendation

The recommendations below provide a starting point for assay optimization. Actual working concentration varies and should be decided by the user.

Western blot, 0.1-0.5μg/ml, Human, -
Immunocytochemistry , 0.5-1μg/ml, Human

Validation Images & Assay Conditions

Gene/Protein Information For EIF2AK3 (Source: Uniprot.Org, NCBI)

Uniprot ID

Q9NZJ5

Gene ID

9451

Gene Name

EIF2AK3

Full Name

Eukaryotic translation initiation factor 2-alpha kinase 3

Weight

125.216kDa

Superfamily

protein kinase superfamily

Alternative Names

DKFZp781H1925; EC 2.7.11.1; EIF2AK3; eukaryotic translation initiation factor 2 alpha kinase 3; eukaryotic translation initiation factor 2-alpha kinase 3; hsPEK; PEK; PEKPERKPancreatic eIF2-alpha kinase; PERK; PRKR-like endoplasmic reticulum kinase; WRS EIF2AK3 PEK, PERK, WRS eukaryotic translation initiation factor 2 alpha kinase 3 eukaryotic translation initiation factor 2-alpha kinase 3|PRKR-like endoplasmic reticulum kinase|pancreatic EIF2-alpha kinase|truncated eukaryotic translation initiation factor 2 alpha kinase 3

*if product is indicated to react with multiple species, protein info is based on the gene entry specified above in "species".

For more info on EIF2AK3, check out the EIF2AK3 Infographic

EIF2AK3 infographic

We have 30,000+ of these available, one for each gene! check them out.

In this infographic you will see the following information for EIF2AK3: database IDs, super-family, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact us [email protected]

PA2026 has been cited in 1 publications:

*The publications in this section are manually curated by our staff scientists. They may differ from Bioz's machine gathered results. Both are accurate. If you find a publication citing this product but is missing from this list, please let us know we will issue you a thank-you coupon.

Recombinant Newcastle disease virus (rL-RVG) triggers autophagy and apoptosis in gastric carcinoma cells by inducing ER stress

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4 Customer Q&As for Anti-PERK/EIF2AK3 Antibody

Question

We have observed staining in human brain. Are there any suggestions? Is anti-PERK/EIF2AK3 antibody supposed to stain brain positively?

Verified Customer

Verified customer

Asked: 2020-01-10

Answer

Based on literature brain does express EIF2AK3. Based on Uniprot.org, EIF2AK3 is expressed in nasal cavity mucosa, liver, pancreas testis, brain pancreas, brain, cervix carcinoma, among other tissues. Regarding which tissues have EIF2AK3 expression, here are a few articles citing expression in various tissues:
Brain, Pubmed ID: 14702039
Brain, and Pancreas, Pubmed ID: 10677345
Cervix carcinoma, Pubmed ID: 20068231
Liver, Pancreas, and Testis, Pubmed ID: 10026192

Boster Scientific Support

Answered: 2020-01-10

Question

Our team were well pleased with the WB result of your anti-PERK/EIF2AK3 antibody. However we have been able to see positive staining in liver endoplasmic reticulum membrane using this antibody. Is that expected? Could you tell me where is EIF2AK3 supposed to be expressed?

Verified Customer

Verified customer

Asked: 2019-10-21

Answer

From literature, liver does express EIF2AK3. Generally EIF2AK3 expresses in endoplasmic reticulum membrane. Regarding which tissues have EIF2AK3 expression, here are a few articles citing expression in various tissues:
Brain, Pubmed ID: 14702039
Brain, and Pancreas, Pubmed ID: 10677345
Cervix carcinoma, Pubmed ID: 20068231
Liver, Pancreas, and Testis, Pubmed ID: 10026192

Boster Scientific Support

Answered: 2019-10-21

Question

We purchased anti-PERK/EIF2AK3 antibody for WB on brain in the past. I am using human, and We intend to use the antibody for ICC next. I was wanting to use examining brain as well as cervix carcinoma in our next experiment. Do you have any suggestion on which antibody would work the best for ICC?

Verified Customer

Verified customer

Asked: 2019-07-30

Answer

I looked at the website and datasheets of our anti-PERK/EIF2AK3 antibody and it seems that PA2026 has been tested on human in both WB and ICC. Thus PA2026 should work for your application. Our Boster satisfaction guarantee will cover this product for ICC in human even if the specific tissue type has not been validated. We do have a comprehensive range of products for ICC detection and you can check out our website bosterbio.com to find out more information about them.

Boster Scientific Support

Answered: 2019-07-30

Question

We are currently using anti-PERK/EIF2AK3 antibody PA2026 for human tissue, and we are happy with the WB results. The species of reactivity given in the datasheet says human. Is it likely that the antibody can work on primate tissues as well?

C. Miller

Verified customer

Asked: 2016-11-28

Answer

The anti-PERK/EIF2AK3 antibody (PA2026) has not been tested for cross reactivity specifically with primate tissues, but there is a good chance of cross reactivity. We have an innovator award program that if you test this antibody and show it works in primate you can get your next antibody for free. Please contact me if I can help you with anything.

Boster Scientific Support

Answered: 2016-11-28

Size

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