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- Table of Contents
EIF2AK3 antibody (A01992-2) performs well in ELISA, IHC, WB and shows reactivity with Human.
- Validated for inter-lot consistency, specificity, and reproducibility
- Committed to quality since 1993
- Over 8 Publications
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IHC analysis of PERK/EIF2AK3 using anti-PERK/EIF2AK3 antibody (A01992-2).
PERK/EIF2AK3 was detected in a paraffin-embedded section of human pancreas cancer tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-PERK/EIF2AK3 Antibody (A01992-2) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.
PERK/EIF2AK3 was detected in a paraffin-embedded section of human pancreas cancer tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-PERK/EIF2AK3 Antibody (A01992-2) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.
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Anti-PERK/EIF2AK3 Antibody Picoband®
EIF2AK3 antibody
| Reactive Species | Host | Application |
|---|---|---|
| Human | Rabbit | ELISA, IHC, WB |
Tested Applications
EIF2AK3 antibody was positively detected by WB in:
human K562 whole cell, human 293T whole cell, human MCF-7 whole cell, human HepG2 whole cell
Suggested blocking solution with 5% non-fat milk or BSA; (*)Recommended protein loading: 20-40 ug per lane
EIF2AK3 antibody was positively detected by IHC in:
human pancreas cancer tissue
Use TE buffer pH 9.0 for antigen retrieval; (*) citrate buffer pH 6.0 is an alternative.
Recommend Dilution
Application
Dilution
Species
Western blot
0.1-0.5μg/ml
Human
Immunohistochemistry (Paraffin-embedded Section)
2-5μg/ml
Human
ELISA
0.1-0.5μg/ml
-
* It is recommended to optimize the amount of this reagent in each experimental system to achieve the best results.
Reactive Data
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WB
IHC
IF
ELISA
Human
Tested
Tested
Recommend
Recommend
Monkey
Predicted
Predicted
Predicted
Predicted
Rabbit
Predicted
Predicted
Predicted
Predicted
Bovine
Predicted
Predicted
Predicted
Predicted
Sheep
Predicted
Predicted
Predicted
Predicted
Product Information
Product Name
Anti-PERK/EIF2AK3 Antibody Picoband®
View all EIF2AK3 Antibodies
View all EIF2AK3 Antibodies
Form
Lyophilized
Uniprot
Gene ID
Storage & Handling
Store at -20˚C for one year from date of receipt. After reconstitution, at 4˚C for one month. It can also be aliquotted and stored frozen at -20˚C for six months. Avoid repeated freeze-thaw cycles.
Host
Rabbit
Contents
Each vial contains 4 mg Trehalose, 0.9 mg NaCl and 0.2 mg Na2HPO4.
Clonality
Polyclonal
Isotype
Rabbit IgG
Immunogen
E. coli-derived human PERK recombinant protein (Position: R222-Q334).
Cross-Reactivity
No cross-reactivity with other proteins.
Reconstitution
Add 0.2ml of distilled water will yield a concentration of 500ug/ml.
Observed Molecular Weight
150 kDa
Calculated Molecular Weight
125.2 kDa
Background of EIF2AK3
Eukaryotic translation initiation factor 2-alpha kinase 3, also known as protein kinase R (PKR)-like endoplasmic reticulum kinase (PERK), is an enzyme that in humans is encoded by the EIF2AK3 gene. The protein encoded by this gene phosphorylates the alpha subunit of eukaryotic translation-initiation factor 2, leading to its inactivation, and thus to a rapid reduction of translational initiation and repression of global protein synthesis. This protein is thought to modulate mitochondrial function. It is a type I membrane protein located in the endoplasmic reticulum (ER), where it is induced by ER stress caused by malfolded proteins. Mutations in this gene are associated with Wolcott-Rallison syndrome.
Cite This Product
Anti-PERK/EIF2AK3 Antibody Picoband® (Boster Biological Technology, Pleasanton CA, USA, Catalog # A01992-2)
Antibody Homology Across Species
The results generated by this homology analysis tool for commonly used animal species are shown below. For unlisted species, please refer to the homology analysis between the immunogen sequence and the corresponding species.
A homology of ≥90% is recommended. Generally, higher homology indicates a greater potential for cross-species reactivity. However, protein expression levels may vary among different species, tissues, and cell types. Therefore, the homology analysis results are for reference only and do not guarantee antibody cross-reactivity or experimental performance.
Human
100%
High Homology
Monkey
99%
High Homology
Rabbit
94%
High Homology
Cow
91%
High Homology
Sheep
90%
High Homology
Mouse
88%
Moderate Homology
Dog
88%
Moderate Homology
Rat
87%
Moderate Homology
Zebrafish
55%
Low homology
Drosophila
35%
Low homology
Homology ≥ 90%High Homology
70% ≤ Homology < 90%Moderate Homology
Homology < 70%Low homology
Protocols
Western Blot protocol for EIF2AK3 antibody
Immunohistochemistry (IHC) protocol for EIF2AK3 antibody
Publications
Application:
Species:
Neuroprotective effects of dexmedetomidine in spinal cord injury via modulating PERK/ATF4 pathway
Published: 2025-07-11
Journal: NEUROLOGICAL RESEARCH
Application: WB
Impact Factor: 1.50
Species: Rat
PMID: 40642804
CD38 in SLE CD4 T cells promotes Ca2+ flux and suppresses interleukin-2 production by enhancing the expression of GM2 on the surface membrane
Published: 2024-09-27
Journal: Nature Communications
Application: FC
Impact Factor: 14.70
Species: Human
PMID: 39333474
ASK1 Enhances Angiotensin II-Induced Liver Fibrosis In Vitro by Mediating Endoplasmic Reticulum Stress-Dependent Exosomes
Published: 2020-11-09
Journal: MEDIATORS OF INFLAMMATION
Application: WB
Impact Factor: 3.76
Species: Human
PMID: 33223956
Total Panax notoginseng saponin inhibits balloon injury-induced neointimal hyperplasia in rat carotid artery models by suppressing pERK/p38 MAPK pathways
Published: 2019-12-20
Journal: BRAZILIAN JOURNAL OF MEDICAL AND BIOLOGICAL RESEARCH
Application: WB,IHC
Impact Factor: 1.85
Species: Rat
PMID: 31859914
miR-9-5p attenuates ischemic stroke through targeting ERMP1-mediated endoplasmic reticulum stress
Published: 2019-09-07
Journal: ACTA HISTOCHEMICA
Application: WB
Impact Factor: 1.72
Species: Human,Rat
PMID: 31500865
Effects of 5‑hydroxy‑4'‑nitro‑7‑propionyloxy‑genistein on inhibiting proliferation and invasion via activating reactive oxygen species in human ovarian cancer A2780/DDP cells
Published: 2018-02-02
Journal: Oncology Letters
Application: WB
Impact Factor: 1.66
Species: Rat
PMID: 29552162
Recombinant Newcastle disease virus (rL-RVG) triggers autophagy and apoptosis in gastric carcinoma cells by inducing ER stress
Published: 2016-05-01
Journal: American Journal of Cancer Research
Application: WB
Impact Factor: 3.43
Species: Human
PMID: 27293989
Endoplasmic reticulum stress in diethylnitrosamine‑induced rat liver cancer
Published: 2013-10-29
Journal: Oncology Letters
Application: WB
Impact Factor: 0.24
Species: Rat
PMID: 24348814
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Product Promise
We promise Anti-PERK/EIF2AK3 Antibody Picoband® performs excellently in relevant applications for ELISA, IHC, WB. If it doesn't perform as promised, we will refund the product. Learn more
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Order Product
A01992-2
Buy one primary antibody get one 0.5ml HRP or Biotin secondary antibody for free.
*Sample sizes are prepared on demand and will take extra lead time. (cannot be conjugated)
$370.00
Troubleshooting
Boster Guarantee
Guaranteed product quality
We promise all of our products perform as described in datasheets.
This product is for Research Use Only. Not for use in diagnostic procedures.
Update date:
Oct 4, 2026