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Product Info Summary
| SKU: | A01992-2 |
|---|---|
| Size: | 100 μg/vial |
| Reactive Species: | Human |
| Host: | Rabbit |
| Application: | ELISA, IHC, WB |
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Product info
Product Name
Anti-PERK/EIF2AK3 Antibody Picoband®
SKU/Catalog Number
A01992-2
Size
100 μg/vial
Form
Lyophilized
Description
Boster Bio Anti-PERK/EIF2AK3 Antibody Picoband® catalog # A01992-2. Tested in ELISA, IHC, WB applications. This antibody reacts with Human. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.
Storage & Handling
Store at -20˚C for one year from date of receipt. After reconstitution, at 4˚C for one month. It can also be aliquotted and stored frozen at -20˚C for six months. Avoid repeated freeze-thaw cycles.
Cite This Product
Anti-PERK/EIF2AK3 Antibody Picoband® (Boster Biological Technology, Pleasanton CA, USA, Catalog # A01992-2)
Host
Rabbit
Contents
Each vial contains 4 mg Trehalose, 0.9 mg NaCl and 0.2 mg Na2HPO4.
Clonality
Polyclonal
Isotype
Rabbit IgG
Immunogen
E. coli-derived human PERK recombinant protein (Position: R222-Q334).
Cross-reactivity
No cross-reactivity with other proteins.
Reactive Species
A01992-2 is reactive to EIF2AK3 in Human
Observed Molecular Weight
150 kDa
Calculated molecular weight
125.2 kDa
Background of EIF2AK3
Eukaryotic translation initiation factor 2-alpha kinase 3, also known as protein kinase R (PKR)-like endoplasmic reticulum kinase (PERK), is an enzyme that in humans is encoded by the EIF2AK3 gene. The protein encoded by this gene phosphorylates the alpha subunit of eukaryotic translation-initiation factor 2, leading to its inactivation, and thus to a rapid reduction of translational initiation and repression of global protein synthesis. This protein is thought to modulate mitochondrial function. It is a type I membrane protein located in the endoplasmic reticulum (ER), where it is induced by ER stress caused by malfolded proteins. Mutations in this gene are associated with Wolcott-Rallison syndrome.
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
A01992-2 is guaranteed for ELISA, IHC, WB Boster Guarantee
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
Western blot, 0.1-0.5μg/ml, Human
Immunohistochemistry (Paraffin-embedded Section), 2-5μg/ml, Human
ELISA, 0.1-0.5μg/ml, -
Positive Control
WB: human K562 whole cell, human 293T whole cell, human MCF-7 whole cell, human HepG2 whole cell
IHC: human pancreas cancer tissue
Validation Images & Assay Conditions
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Western blot analysis of PERK/EIF2AK3 using anti-PERK/EIF2AK3 antibody (A01992-2).
Electrophoresis was performed on a 8% SDS-PAGE gel at 80V (Stacking gel) / 120V (Resolving gel) for 2 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions.
Lane 1: human K562 whole cell lysates,
Lane 2: human 293T whole cell lysates,
Lane 3: human MCF-7 whole cell lysates,
Lane 4: human HepG2 whole cell lysates.
After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-PERK/EIF2AK3 antigen affinity purified polyclonal antibody (A01992-2) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an ECL Plus Western Blotting Substrate (Catalog # AR1196-200) with Tanon 5200 system. A specific band was detected for PERK/EIF2AK3 at approximately 150 kDa. The expected band size for PERK/EIF2AK3 is at 125 kDa.
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IHC analysis of PERK/EIF2AK3 using anti-PERK/EIF2AK3 antibody (A01992-2).
PERK/EIF2AK3 was detected in a paraffin-embedded section of human pancreas cancer tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-PERK/EIF2AK3 Antibody (A01992-2) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.
Specific Publications For Anti-PERK/EIF2AK3 Antibody Picoband® (A01992-2)
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Customer Q&As
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5 Customer Q&As for Anti-PERK/EIF2AK3 Antibody Picoband®
Question
We are currently using anti-PERK/EIF2AK3 antibody A01992-2 for mouse tissue, and we are satisfied with the Flow Cytometry results. The species of reactivity given in the datasheet says human, mouse, rat. Is it likely that the antibody can work on canine tissues as well?
Verified Customer
Verified customer
Asked: 2019-08-27
Answer
The anti-PERK/EIF2AK3 antibody (A01992-2) has not been tested for cross reactivity specifically with canine tissues, though there is a good chance of cross reactivity. We have an innovator award program that if you test this antibody and show it works in canine you can get your next antibody for free. Please contact me if I can help you with anything.
Boster Scientific Support
Answered: 2019-08-27
Question
We have observed staining in mouse nasal cavity mucosa. Any tips? Is anti-PERK/EIF2AK3 antibody supposed to stain nasal cavity mucosa positively?
K. Li
Verified customer
Asked: 2019-08-21
Answer
From literature nasal cavity mucosa does express EIF2AK3. From Uniprot.org, EIF2AK3 is expressed in nasal cavity mucosa, liver, pancreas testis, brain pancreas, brain, cervix carcinoma, among other tissues. Regarding which tissues have EIF2AK3 expression, here are a few articles citing expression in various tissues:
Brain, Pubmed ID: 14702039
Brain, and Pancreas, Pubmed ID: 10677345
Cervix carcinoma, Pubmed ID: 20068231
Liver, Pancreas, and Testis, Pubmed ID: 10026192
Boster Scientific Support
Answered: 2019-08-21
Question
I was wanting to use using your anti-PERK/EIF2AK3 antibody for bone mineralization studies. Has this antibody been tested with western blotting on hepg2 cells? We would like to see some validation images before ordering.
Verified Customer
Verified customer
Asked: 2019-06-03
Answer
Thank you for your inquiry. This A01992-2 anti-PERK/EIF2AK3 antibody is validated on human hela, hela whole cell lysates, a549 whole cell lysates, a431 whole cell lysates, rat brain tissue, mouse brain, hepg2 cells. It is guaranteed to work for ELISA, Flow Cytometry, WB in human, mouse, rat. Our Boster guarantee will cover your intended experiment even if the sample type has not been be directly tested.
Boster Scientific Support
Answered: 2019-06-03
Question
We bought anti-PERK/EIF2AK3 antibody for ELISA on nasal cavity mucosa in the past. I am using rat, and I plan to use the antibody for Flow Cytometry next. I am interested in examining nasal cavity mucosa as well as liver in our next experiment. Could give a recommendation on which antibody would work the best for Flow Cytometry?
Verified Customer
Verified customer
Asked: 2019-05-27
Answer
I looked at the website and datasheets of our anti-PERK/EIF2AK3 antibody and it seems that A01992-2 has been validated on rat in both ELISA and Flow Cytometry. Thus A01992-2 should work for your application. Our Boster satisfaction guarantee will cover this product for Flow Cytometry in rat even if the specific tissue type has not been validated. We do have a comprehensive range of products for Flow Cytometry detection and you can check out our website bosterbio.com to find out more information about them.
Boster Scientific Support
Answered: 2019-05-27
Question
Our team were well pleased with the WB result of your anti-PERK/EIF2AK3 antibody. However we have been able to see positive staining in nasal cavity mucosa endoplasmic reticulum membrane using this antibody. Is that expected? Could you tell me where is EIF2AK3 supposed to be expressed?
T. Wu
Verified customer
Asked: 2019-01-29
Answer
From literature, nasal cavity mucosa does express EIF2AK3. Generally EIF2AK3 expresses in endoplasmic reticulum membrane. Regarding which tissues have EIF2AK3 expression, here are a few articles citing expression in various tissues:
Brain, Pubmed ID: 14702039
Brain, and Pancreas, Pubmed ID: 10677345
Cervix carcinoma, Pubmed ID: 20068231
Liver, Pancreas, and Testis, Pubmed ID: 10026192
Boster Scientific Support
Answered: 2019-01-29
