Click image to see more details
Product Info Summary
| SKU: | A03926 |
|---|---|
| Size: | 0.1 mg |
| Reactive Species: | Human |
| Host: | Rabbit |
| Application: | ELISA, ICC, WB |
Customers Who Bought This Also Bought
Product info
Product Name
Anti-PERP Antibody
SKU/Catalog Number
A03926
Size
0.1 mg
Form
Liquid
Description
Boster Bio Anti-PERP Antibody (Catalog # A03926). Tested in ELISA, WB, ICC applications. This antibody reacts with Human.
Storage & Handling
PERP antibody can be stored at 4°C for three months and -20°C, stable for up to one year. Avoid repeated freeze-thaw cycles. Antibodies should not be exposed to prolonged high temperatures.
Cite This Product
Anti-PERP Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # A03926)
Host
Rabbit
Contents
PERP Antibody is supplied in PBS containing 0.02% sodium azide.
Clonality
Polyclonal
Isotype
IgG
Immunogen
Anti-PERP antibody was raised against a peptide corresponding to 17 amino acids near the carboxy terminus of human PERP. The immunogen is located within the last 50 amino acids of PERP.
Reactive Species
A03926 is reactive to PERP in Human
Observed Molecular Weight
68 kDa
Calculated molecular weight
21.6 kDa
Background of PERP
The p53 tumor-suppressor gene integrates numerous signals that control cell life and death. Several novel molecules involved in p53 network, including Chk2, p53R2, p53AIP1, Noxa, PIDD, PID/MTA2, MTBP and PERP, were identified and their genes were cloned recently. PERP, also termed PIGPC1 and THW, is a plasma membrane protein. p53 binds to the promoter of PERP and transcriptionally activates PERP gene then the translated PERP protein mediates the p53 induced apoptosis. The expression of PERP causes cell death. PERP is a mediator of p53 induced apoptosis. PERP has sequence similarity to PMP-22/gas3 and is a new member of the PMP-22/gas3 family.
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
A03926 is guaranteed for ELISA, ICC, WB Boster Guarantee
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
WB: 1-2 μg/mL; ICC: 10 μg/mL.
Antibody validated: Western Blot in human samples and Immunocytochemistry in human samples. All other applications and species not yet tested. Optimal dilutions for each application should be determined by the researcher.
Validation Images & Assay Conditions
Click image to see more details
Independent Antibody Validation (IAV) via Protein Expression Profile in Human Cell Lines
Loading: 15 μg of lysates per lane.
Antibodies: PERP A03926 (1 μg/mL), PERP, 57-777 (2 μg/mL), beta-actin 3779 (1 μg/mL) and GAPDH (0.02 μg/mL), 1h incubation at RT in 5% NFDM/TBST.
Secondary: Goat anti-rabbit IgG HRP conjugate at 1:10000 dilution.
Click image to see more details
Western Blot Validation in Human Cell Lines
Loading: 15 μg of lysates per lane.
Antibodies: PERP A03926 (1 μg/mL), 1h incubation at RT in 5% NFDM/TBST.
Secondary: Goat anti-rabbit IgG HRP conjugate at 1:10000 dilution.
Click image to see more details
Western Blot Validation in Human A431 whole cell lysates in the Absence (A) and Presence (B) of Blocking Peptide
Loading: 15 μg of lysates per lane.
Antibodies: PERP A03926 (1 μg/mL), 1h incubation at RT in 5% NFDM/TBST.
Secondary: Goat anti-rabbit IgG HRP conjugate at 1:10000 dilution.
Click image to see more details
Immunocytochemistry Validation of PERP in A431 Cells
Immunocytochemical analysis of A431 cells using anti-PEPR antibody (A03926) at 10 μg/ml. Cells was fixed with formaldehyde and blocked with 10% serum for 1 h at RT; antigen retrieval was by heat mediation with a citrate buffer (pH6). Samples were incubated with primary antibody overnight at 4˚C. A goat anti-rabbit IgG H&L (HRP) at 1/250 was used as secondary. Counter stained with Hematoxylin.
Specific Publications For Anti-PERP Antibody (A03926)
Loading publications
Recommended Resources
Here are featured tools and databases that you might find useful.
- Boster's Pathways Library
- Protein Databases
- Bioscience Research Protocol Resources
- Data Processing & Analysis Software
- Photo Editing Software
- Scientific Literature Resources
- Research Paper Management Tools
- Molecular Biology Software
- Primer Design Tools
- Bioinformatics Tools
- Phylogenetic Tree Analysis
Customer Reviews
Have you used Anti-PERP Antibody?
Share your experimental results or join a short interview to earn up to $1,000 in product credits or other rewards.
0 Reviews For Anti-PERP Antibody
Customer Q&As
Have a question?
Find answers in Q&As, reviews.
Can't find your answer?
Submit your question
