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Product Info Summary
| SKU: | M03926 |
|---|---|
| Size: | 100 μl |
| Reactive Species: | Human, Mouse, Rat |
| Host: | Rabbit |
| Application: | IF, ICC, WB |
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Product info
Product Name
Anti-PERP Monoclonal Antibody
SKU/Catalog Number
M03926
BM5707 is an alternative SKU for this antibody, used in previous lots.
Size
100 μl
Form
Liquid
Description
Boster Bio Anti-PERP Monoclonal Antibody catalog # M03926. Tested in WB, ICC/IF applications. This antibody reacts with Human, Mouse, Rat.
Storage & Handling
Store at -20°C for one year. For short term storage and frequent use, store at 4°C for up to one month. Avoid repeated freeze-thaw cycles.
Cite This Product
Anti-PERP Monoclonal Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # M03926)
Host
Rabbit
Contents
Rabbit IgG in stabilizing components, phosphate buffered saline, pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
*This antibody is supplied in a stabilized formulation.
Compatibility with conjugation reactions depends on the chemistry of the conjugation method used.
For conjugation methods that are not compatible with the stabilizing components present in this formulation, a carrier-free antibody format is required.
Clonality
Monoclonal
Clone Number
AFDD-16
Isotype
Rabbit IgG
Immunogen
A synthesized peptide derived from human PERP
Reactive Species
M03926 is reactive to PERP in Human, Mouse, Rat
Observed Molecular Weight
21 kDa
Calculated molecular weight
21.4 kDa
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
M03926 is guaranteed for IF, ICC, WB Boster Guarantee
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
WB 1:500-2000
ICC/IF 1:50-200
Positive Control
WB: A431 cell
Validation Images & Assay Conditions
Click image to see more details
Western blot analysis of PERP expression in A431 cell lysate.
Specific Publications For Anti-PERP Monoclonal Antibody (M03926)
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Customer Q&As
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7 Customer Q&As for Anti-PERP Monoclonal Antibody
Question
Would anti-PERP Monoclonal antibody M03926 work for ICC with pancreas?
Verified Customer
Verified customer
Asked: 2020-03-12
Answer
According to the expression profile of pancreas, PERP is highly expressed in pancreas. So, it is likely that anti-PERP Monoclonal antibody M03926 will work for ICC with pancreas.
Boster Scientific Support
Answered: 2020-03-12
Question
What is the amount of the biotin or HRP secondary antibody for the M03926?
Verified customer
Asked: 2019-09-12
Answer
For the Anti-PERP Monoclonal Antibody (M03926) secondary antibody would be the smallest size 0.5ml.
Boster Scientific Support
Answered: 2019-09-12
Question
Would M03926 anti-PERP Monoclonal antibody work on parafin embedded sections? If so, which fixation method do you recommend we use (PFA, paraformaldehyde, other)?
Verified Customer
Verified customer
Asked: 2019-09-10
Answer
You can see on the product datasheet, M03926 anti-PERP Monoclonal antibody as been validated on ICC. It is best to use PFA for fixation because it has better tissue penetration ability. PFA needs to be prepared fresh before use. Long term stored PFA turns into formalin, as the PFA molecules congregate and become formalin.
Boster Scientific Support
Answered: 2019-09-10
Question
Thanks for helping with my inquiry over the phone. Here are the WB image, lot number and protocol we used for pancreas using anti-PERP Monoclonal antibody M03926. Let me know if you need anything else.
Verified Customer
Verified customer
Asked: 2019-09-03
Answer
We appreciate the data. You have provided everything we needed. Our lab team are working to resolve your inquiry as quickly as possible, and we appreciate your patience and understanding! Please let me know if there is anything you need in the meantime.
Boster Scientific Support
Answered: 2019-09-03
Question
I was wanting to use your anti-PERP Monoclonal antibody for ICC for human pancreas on frozen tissues, but I want to know if it has been tested for this particular application. Has this antibody been tested and is this antibody a good choice for human pancreas identification?
Z. Wu
Verified customer
Asked: 2017-10-30
Answer
It shows on the product datasheet, M03926 anti-PERP Monoclonal antibody has been validated for IF, ICC, WB on human, mouse, rat tissues. We have an innovator award program that if you test this antibody and show it works in human pancreas in IHC-frozen, you can get your next antibody for free.
Boster Scientific Support
Answered: 2017-10-30
Question
We are currently using anti-PERP Monoclonal antibody M03926 for mouse tissue, and we are satisfied with the IF results. The species of reactivity given in the datasheet says human, mouse, rat. Is it likely that the antibody can work on goat tissues as well?
B. Singh
Verified customer
Asked: 2016-07-15
Answer
The anti-PERP Monoclonal antibody (M03926) has not been tested for cross reactivity specifically with goat tissues, but there is a good chance of cross reactivity. We have an innovator award program that if you test this antibody and show it works in goat you can get your next antibody for free. Please contact me if I can help you with anything.
Boster Scientific Support
Answered: 2016-07-15
Question
Does anti-PERP Monoclonal antibody M03926 work on horse IF with keratinocyte?
C. Li
Verified customer
Asked: 2015-12-08
Answer
Our lab technicians have not validated anti-PERP Monoclonal antibody M03926 on horse. You can run a BLAST between horse and the immunogen sequence of anti-PERP Monoclonal antibody M03926 to see if they may cross-react. If the sequence homology is close, then you can perform a pilot test. Keep in mind that since we have not validated horse samples, this use of the antibody is not covered by our guarantee. However we have an innovator award program that if you test this antibody and show it works in horse keratinocyte in IF, you can get your next antibody for free.
Boster Scientific Support
Answered: 2015-12-08
