Anti-Platelet factor 4 PF4 Antibody

Product Name

Anti-Platelet factor 4 PF4 Antibody

View all CXCL4/PF4 Antibodies

SKU/Catalog Number

PA1446

Size

100 μg/vial

Form

Lyophilized

Description

Boster Bio Anti-Platelet factor 4 PF4 Antibody catalog # PA1446. Tested in IHC, WB applications. This antibody reacts with Human.

Storage & Handling

Store at -20˚C for one year from date of receipt. After reconstitution, at 4˚C for one month. It can also be aliquotted and stored frozen at -20˚C for six months. Avoid repeated freeze-thaw cycles.

Cite This Product

Anti-Platelet factor 4 PF4 Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # PA1446)

Host

Rabbit

Contents

Each vial contains 5mg BSA, 0.9mg NaCl, 0.2mg Na2HPO4, 0.05mg Thimerosal, 0.05mg NaN3.

Clonality

Polyclonal

Isotype

Rabbit IgG

Immunogen

A synthetic peptide corresponding to a sequence at the C-terminus of human PF4.

*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.

Cross-reactivity

No cross-reactivity with other proteins

Reactive Species

PA1446 is reactive to PF4 in Human

Applications

PA1446 is guaranteed for IHC, WB Boster Guarantee

Observed Molecular Weight

50 kDa

Calculated molecular weight

10.845kDa

Background of CXCL4/PF4

CXCL4, Chemokine (C-X-C motif) ligand 4, is a 70-amino acid protein that is released from the alpha-granules of activated platelets and binds with high affinity to heparin. CXCL4 belongs to the CXC chemokine family. The CXCL4 gene contains 3 exons spanning approximately 1 kb. The gene is encoded on a 10-kb EcoRI fragment of genomic DNA. By in situ hybridization, the CXCL4 gene is mapped to chromosome 4q12-q21. Its major physiologic role appears to be neutralization of heparin-like molecules on the endothelial surface of blood vessels, thereby inhibiting local antithrombin III activity and promoting coagulation. As a strong chemoattractant for neutrophils and fibroblasts, PF4 probably has a role in inflammation and wound repair.

Antibody Validation

Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.

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Reconsitution

Add 0.2ml of distilled water will yield a concentration of 500ug/ml.

Assay Dilutions Recommendation

The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.

Immunohistochemistry (Paraffin-embedded Section), 0.5-1μg/ml, Human, By Heat
Western blot, 0.1-0.5μg/ml, Human

Validation Images & Assay Conditions

Click image to see more details

Anti-PF4 antibody, PA1446, Western blotting
Lane 1: Recombinant Human CXCL4 Protein 10ng
Lane 2: Recombinant Human CXCL4 Protein 5ng
Lane 3: Recombinant Human CXCL4 Protein 2.5ng

Click image to see more details

Anti-PF4 antibody, PA1446, IHC(P)
IHC(P): Human Lung Cancer Tissue

Gene/Protein Information For PF4 (Source: Uniprot.org, NCBI)

Alternative Names

chemokine (C-X-C motif) ligand 4; C-X-C motif chemokine 4; CXCL4; CXCL4iroplact; Iroplact; MGC138298; Oncostatin-A; PF4; PF-4; platelet factor 4; SCYB4oncostatin-A PF4 CXCL4, PF-4, SCYB4 platelet factor 4 platelet factor 4|C-X-C motif chemokine 4|chemokine (C-X-C motif) ligand 4|iroplact|oncostatin-A

For more info on PF4, check out the PF4 Infographic

We have 30,000+ of these available, one for each gene! Check them out.

In this infographic, you will see the following information for PF4: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].

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