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PFAS antibody (M03795) performs well in IF, ICC, WB and shows reactivity with Human, Mouse, Rat.
- Validated for inter-lot consistency, specificity, and reproducibility
- Committed to quality since 1993
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IF analysis of PFAS using anti-PFAS antibody (M03795).
PFAS was detected in an immunocytochemical section of A549 cells. Enzyme antigen retrieval was performed using IHC enzyme antigen retrieval reagent (AR0022) for 15 mins. The cells were blocked with 10% goat serum. And then incubated with 2 μg/ml mouse anti-PFAS Antibody (M03795) overnight at 4°C. Fluoro594 Conjugated Goat Anti-Mouse IgG (BA1141) was used as secondary antibody at 1:500 dilution and incubated for 30 minutes at 37°C. Visualize using a fluorescence microscope and filter sets appropriate for the label used.
PFAS was detected in an immunocytochemical section of A549 cells. Enzyme antigen retrieval was performed using IHC enzyme antigen retrieval reagent (AR0022) for 15 mins. The cells were blocked with 10% goat serum. And then incubated with 2 μg/ml mouse anti-PFAS Antibody (M03795) overnight at 4°C. Fluoro594 Conjugated Goat Anti-Mouse IgG (BA1141) was used as secondary antibody at 1:500 dilution and incubated for 30 minutes at 37°C. Visualize using a fluorescence microscope and filter sets appropriate for the label used.
Designing a Western blot for PFAS? The guide covers the expected band size, antibodies backed by real blot images, the controls to run alongside, and protocols taken from published papers.
Open the PFAS Western Blot GuideBundle Save
Anti-PFAS Antibody Picoband® (monoclonal, 3D12D8)
PFAS antibody
| Reactive Species | Host | Application |
|---|---|---|
| Human, Mouse, Rat | Mouse | IF, ICC, WB |
Tested Applications
PFAS antibody was positively detected by WB in:
human A549 whole cell, human 293T whole cell, human Hela whole cell, human HepG2 whole cell, rat liver tissue, rat stomach tissue, mouse liver tissue
Suggested blocking solution with 5% non-fat milk or BSA; (*)Recommended protein loading: 20-40 ug per lane
PFAS antibody was positively detected by ICC/IF in:
A549 cell
Recommend Dilution
Application
Dilution
Species
Western blot
0.1-0.5 μg/ml
Human, Mouse, Rat
Immunohistochemistry(Paraffin-embedded Section)
2-5 μg/ml
Human
* It is recommended to optimize the amount of this reagent in each experimental system to achieve the best results.
Product Information
Product Name
Anti-PFAS Antibody Picoband® (monoclonal, 3D12D8)
View all PFAS Antibodies
View all PFAS Antibodies
Form
Lyophilized
Uniprot
Gene ID
Storage & Handling
At -20℃ for one year from date of receipt. After reconstitution, at 4℃ for one month. It can also be aliquotted and stored frozen at -20℃ for six months. Avoid repeated freezing and thawing.
Host
Mouse
Contents
Each vial contains 4 mg Trehalose, 0.9 mg NaCl and 0.2 mg Na2HPO4.
Clonality
Monoclonal
Clone Number
3D12D8
Isotype
IgG2b
Immunogen
E. coli-derived human PFAS recombinant protein (Position: R330-S569). Human PFAS shares 96.7% amino acid (aa) sequence identity with both mouse and rat PFAS.
Reconstitution
Adding 0.2 ml of distilled water will yield a concentration of 500 μg/ml.
Observed Molecular Weight
145 kDa
Calculated Molecular Weight
144.7 kDa
Background of PFAS
Purines are necessary for many cellular processes, including DNA replication, transcription, and energy metabolism. Ten enzymatic steps are required to synthesize inosine monophosphate (IMP) in the de novo pathway of purine biosynthesis. The enzyme encoded by this gene catalyzes the fourth step of IMP biosynthesis.
Cite This Product
Anti-PFAS Antibody Picoband® (monoclonal, 3D12D8) (Boster Biological Technology, Pleasanton CA, USA, Catalog # M03795)
Protocols
Western Blot protocol for PFAS antibody
Immunocytochemistry/Immunofluorescence (ICC/IF) protocol for PFAS antibody
Review
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Product Promise
We promise Anti-PFAS Antibody Picoband® (monoclonal, 3D12D8) performs excellently in relevant applications for IF, ICC, WB. If it doesn't perform as promised, we will refund the product. Learn more
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M03795
Buy one primary antibody get one 0.5ml HRP or Biotin secondary antibody for free.
*Sample sizes are prepared on demand and will take extra lead time. (cannot be conjugated)
$370.00
Troubleshooting
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We promise all of our products perform as described in datasheets.
This product is for Research Use Only. Not for use in diagnostic procedures.
Update date:
Oct 18, 2026