Click image to see more details
Product Info Summary
| SKU: | A30501 |
|---|---|
| Size: | 100ul |
| Reactive Species: | Human, Mouse, Rat |
| Host: | Rabbit |
| Application: | ELISA, IHC, WB |
Customers Who Bought This Also Bought
Product info
Product Name
Anti-PFKFB1/4 Antibody
SKU/Catalog Number
A30501
Size
100ul
Form
Liquid
Description
Boster Bio Anti-PFKFB1/4 Antibody (Catalog# A30501). Tested in WB, ELISA, IHC applications. This antibody reacts with Human, Mouse, Rat.
Storage & Handling
Store at -20°C for one year. For short-term storage and frequent use, store at 4°C for up to one month. Avoid repeated freeze-thaw cycles.
Cite This Product
Anti-PFKFB1/4 Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # A30501)
Host
Rabbit
Contents
PBS containing 50% glycerol, 0.5% stabilizing protein and 0.02% sodium azide.
This antibody is supplied in a stabilized formulation.
Compatibility with conjugation reactions depends on the chemistry of the conjugation method used.
For conjugation methods that are not compatible with the stabilizing components present in this formulation, a carrier-free antibody format is required.
Clonality
Polyclonal
Isotype
Rabbit IgG
Immunogen
A peptide derived from human PFKFB1/4. Immunogen sequence location: 331-380
Reactive Species
A30501 is reactive to PFKFB1 in Human, Mouse, Rat
Observed Molecular Weight
72 kDa
Calculated molecular weight
54.7 kDa
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
A30501 is guaranteed for ELISA, IHC, WB Boster Guarantee
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
WB 1:500-2000
IHC 1:50-300
ELISA 2000-20000
Validation Images & Assay Conditions
Click image to see more details
Immunohistochemical analysis of paraffin-embedded human Breast cancer. 1, Antibody was diluted at 1:200(4° overnight). 2, Tris-EDTA,pH9.0 was used for antigen retrieval. 3,Secondary antibody was diluted at 1:200(room temperature, 45min).
Click image to see more details
Western Blot analysis of various cells using PFK-2 liv/tes Polyclonal Antibody
Click image to see more details
Western blot analysis of lysates from HeLa, HepG2, COLO205, and 293 cells, using PFKFB1/4 Antibody. The lane on the right is blocked with the synthesized peptide.
Specific Publications For Anti-PFKFB1/4 Antibody (A30501)
Loading publications
Recommended Resources
Here are featured tools and databases that you might find useful.
- Boster's Pathways Library
- Protein Databases
- Bioscience Research Protocol Resources
- Data Processing & Analysis Software
- Photo Editing Software
- Scientific Literature Resources
- Research Paper Management Tools
- Molecular Biology Software
- Primer Design Tools
- Bioinformatics Tools
- Phylogenetic Tree Analysis
Customer Reviews
Have you used Anti-PFKFB1/4 Antibody?
Share your experimental results or join a short interview to earn up to $1,000 in product credits or other rewards.
0 Reviews For Anti-PFKFB1/4 Antibody
Customer Q&As
Have a question?
Find answers in Q&As, reviews.
Can't find your answer?
Submit your question
5 Customer Q&As for Anti-PFKFB1/4 Antibody
Question
Is a blocking peptide available for product anti-PFKFB1/4 antibody (A30501)?
Verified Customer
Verified customer
Asked: 2020-01-15
Answer
We do provide the blocking peptide for product anti-PFKFB1/4 antibody (A30501). If you would like to place an order for it please contact support@bosterbio.com and make a special request.
Boster Scientific Support
Answered: 2020-01-15
Question
We are currently using anti-PFKFB1/4 antibody A30501 for rat tissue, and we are happy with the WB results. The species of reactivity given in the datasheet says human, mouse, rat. Is it true that the antibody can work on horse tissues as well?
Verified Customer
Verified customer
Asked: 2019-06-03
Answer
The anti-PFKFB1/4 antibody (A30501) has not been tested for cross reactivity specifically with horse tissues, though there is a good chance of cross reactivity. We have an innovator award program that if you test this antibody and show it works in horse you can get your next antibody for free. Please contact me if I can help you with anything.
Boster Scientific Support
Answered: 2019-06-03
Question
I was wanting to use your anti-PFKFB1/4 antibody for WB for mouse liver on frozen tissues, but I want to know if it has been tested for this particular application. Has this antibody been tested and is this antibody a good choice for mouse liver identification?
Verified Customer
Verified customer
Asked: 2019-05-01
Answer
As indicated on the product datasheet, A30501 anti-PFKFB1/4 antibody has been tested for WB on human, mouse, rat tissues. We have an innovator award program that if you test this antibody and show it works in mouse liver in IHC-frozen, you can get your next antibody for free.
Boster Scientific Support
Answered: 2019-05-01
Question
Thanks for helping with my inquiry over the phone. Here are the WB image, lot number and protocol we used for liver using anti-PFKFB1/4 antibody A30501. Let me know if you need anything else.
Verified Customer
Verified customer
Asked: 2019-04-26
Answer
Thank you for the data. You have provided everything we needed. Our lab team are working to resolve your inquiry as quickly as possible, and we appreciate your patience and understanding! Please let me know if there is anything you need in the meantime.
Boster Scientific Support
Answered: 2019-04-26
Question
I see that the anti-PFKFB1/4 antibody A30501 works with WB, what is the protocol used to produce the result images on the product page?
H. Bhatt
Verified customer
Asked: 2016-03-07
Answer
You can find protocols for WB on the "support/technical resources" section of our navigation menu. If you have any further questions, please send an email to support@bosterbio.com
Boster Scientific Support
Answered: 2016-03-07
