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Product Info Summary
| SKU: | M02382 |
|---|---|
| Size: | 100 μl |
| Reactive Species: | Human, Mouse, Rat |
| Host: | Rabbit |
| Application: | Flow Cytometry, IP, IF, IHC, ICC, WB |
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Product info
Product Name
Anti-PFKFB3 Monoclonal Antibody
SKU/Catalog Number
M02382
BM5472 is an alternative SKU for this antibody, used in previous lots.
Size
100 μl
Form
Liquid
Description
Boster Bio Anti-PFKFB3 Monoclonal Antibody catalog # M02382. Tested in WB, IHC, ICC/IF, IP, Flow Cytometry applications. This antibody reacts with Human, Mouse, Rat.
Storage & Handling
Store at -20°C for one year. For short term storage and frequent use, store at 4°C for up to one month. Avoid repeated freeze-thaw cycles.
Cite This Product
Anti-PFKFB3 Monoclonal Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # M02382)
Host
Rabbit
Contents
Rabbit IgG in stabilizing components, phosphate buffered saline, pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
*This antibody is supplied in a stabilized formulation.
Compatibility with conjugation reactions depends on the chemistry of the conjugation method used.
For conjugation methods that are not compatible with the stabilizing components present in this formulation, a carrier-free antibody format is required.
Clonality
Monoclonal
Clone Number
ADII-16
Isotype
Rabbit IgG
Immunogen
A synthesized peptide derived from human PFKFB3.
Reactive Species
M02382 is reactive to PFKFB3 in Human, Mouse, Rat
Observed Molecular Weight
58 kDa
Calculated molecular weight
59.6 kDa
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
M02382 is guaranteed for Flow Cytometry, IP, IF, IHC, ICC, WB Boster Guarantee
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
WB 1:500-2000
IHC 1:50-200
ICC/IF 1:50-200
IP 1:50
FC 1:50
Positive Control
WB:human Hela whole cell, human MCF-7 whole cell, human A549 whole cell, human U251 whole cell
Validation Images & Assay Conditions
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Immunofluorescent analysis using the Antibody at 1:150 dilution.
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Western blot analysis of PFKFB3 using anti-PFKFB3 antibody (M02382).
Electrophoresis was performed on a 10% SDS-PAGE gel at 80V (Stacking gel) / 120V (Resolving gel) for 2 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions.
Lane 1: human Hela whole cell lysates,
Lane 2: human MCF-7 whole cell lysates,
Lane 3: human A549 whole cell lysates,
Lane 4: human U251 whole cell lysates.
After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-PFKFB3 antigen affinity purified monoclonal antibody (M02382) at 1:500 overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an ECL Plus Western Blotting Substrate (Catalog # AR1196-200) with Tanon 5200 system. A specific band was detected for PFKFB3 at approximately 58 kDa. The expected band size for PFKFB3 is at 60 kDa.
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Immunofluorescent analysis using the Antibody at 1:50 dilution.
Specific Publications For Anti-PFKFB3 Monoclonal Antibody (M02382)
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2 Customer Q&As for Anti-PFKFB3 Monoclonal Antibody
Question
We are currently using anti-PFKFB3 Monoclonal antibody M02382 for rat tissue, and we are happy with the ICC results. The species of reactivity given in the datasheet says human, mouse, rat. Is it true that the antibody can work on monkey tissues as well?
Verified Customer
Verified customer
Asked: 2020-01-20
Answer
The anti-PFKFB3 Monoclonal antibody (M02382) has not been validated for cross reactivity specifically with monkey tissues, but there is a good chance of cross reactivity. We have an innovator award program that if you test this antibody and show it works in monkey you can get your next antibody for free. Please contact me if I can help you with anything.
Boster Scientific Support
Answered: 2020-01-20
Question
What sample was tested in WB and IHC applications with M02382?
Verified customer
Asked: 2020-01-09
Answer
Western blot analysis of PFKFB3 expression in Jurkat cell lysate.
Boster Scientific Support
Answered: 2020-01-09
