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Product Info Summary
| SKU: | A13413 |
|---|---|
| Size: | 0.1 mg |
| Reactive Species: | Human, Mouse, Rat |
| Host: | Rabbit |
| Application: | ELISA, IF, WB |
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Product info
Product Name
Anti-PHD finger protein 14 PHF14 Antibody
SKU/Catalog Number
A13413
Size
0.1 mg
Form
Liquid
Description
Boster Bio Anti-PHD finger protein 14 PHF14 Antibody (Catalog # A13413). Tested in ELISA, WB, IF applications. This antibody reacts with Human, Mouse, Rat.
Storage & Handling
PHF14 antibody can be stored at 4°C for three months and -20°C, stable for up to one year.
Cite This Product
Anti-PHD finger protein 14 PHF14 Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # A13413)
Host
Rabbit
Contents
PHF14 Antibody is supplied in PBS containing 0.02% sodium azide.
Clonality
Polyclonal
Isotype
IgG
Immunogen
PHF14 antibody was raised against a 19 amino acid peptide near the amino terminus of human PHF14. The immunogen is located within amino acids 210 - 260 of PHF14 .
Cross-reactivity
PHF14 antibody is human, mouse and rat reactive. Multiple isoforms of PHF14 are known to exist.
Reactive Species
A13413 is reactive to PHF14 in Human, Mouse, Rat
Observed Molecular Weight
68 kDa
Calculated molecular weight
100.1 kDa
Background of PHF14
PHF14, a novel plant homology domain (PHD) transcription factor, controls the proliferation of mesenchymal cells by regulating PDGFR alpha expression in embryonic development (1). PHD finger transcription factors are concerned with transcriptional regulation by interacting with modified histones (2), and dysregulation of several genes encoding PHD finger transcription factors, such as ING1 and RBP2, has been found in human cancer diseases suggesting these factors have an important role for cancer cell proliferation. PHF14 is a potential target for new treatments of lung fibrosis (1, 3).
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
A13413 is guaranteed for ELISA, IF, WB Boster Guarantee
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
PHF14 antibody can be used for detection of PHF14 by Western blot at 1 - 2 μg/ml.
Antibody validated: Western Blot in human samples and Immunofluorescence in human samples. All other applications and species not yet tested. Optimal dilutions for each application should be determined by the researcher.
Validation Images & Assay Conditions
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Western blot analysis of PHF14 in 293 cell lysate with PHF14 antibody at 1 μg/ml.
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Immunofluorescence of PHF14 in 293 cells with PHF14 antibody at 5 μg/mL.
Specific Publications For Anti-PHD finger protein 14 PHF14 Antibody (A13413)
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