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Product Info Summary
| SKU: | M03015 |
|---|---|
| Size: | 100 μl |
| Reactive Species: | Human, Mouse, Rat |
| Host: | Rabbit |
| Application: | Flow Cytometry, IF, IHC, ICC, WB |
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Product info
Product Name
Anti-PHD1/prolyl hydroxylase Rabbit Monoclonal Antibody
SKU/Catalog Number
M03015
BM3977 is an alternative SKU for this antibody, used in previous lots.
Size
100 μl
Form
Liquid
Description
Boster Bio Anti-PHD1/prolyl hydroxylase Rabbit Monoclonal Antibody catalog # M03015. Tested in WB, IHC, ICC/IF, Flow Cytometry applications. This antibody reacts with Human, Mouse, Rat.
Storage & Handling
Store at -20°C for one year. For short term storage and frequent use, store at 4°C for up to one month. Avoid repeated freeze-thaw cycles.
Cite This Product
Anti-PHD1/prolyl hydroxylase Rabbit Monoclonal Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # M03015)
Host
Rabbit
Contents
Rabbit IgG in stabilizing components, phosphate buffered saline, pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
*This antibody is supplied in a stabilized formulation.
Compatibility with conjugation reactions depends on the chemistry of the conjugation method used.
For conjugation methods that are not compatible with the stabilizing components present in this formulation, a carrier-free antibody format is required.
Clonality
Monoclonal
Clone Number
ABD-5
Isotype
Rabbit IgG
Immunogen
A synthesized peptide derived from human PHD1/prolyl hydroxylase
Reactive Species
M03015 is reactive to EGLN2 in Human, Mouse, Rat
Observed Molecular Weight
48 kDa
Calculated molecular weight
43.7 kDa
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
M03015 is guaranteed for Flow Cytometry, IF, IHC, ICC, WB Boster Guarantee
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
WB 1:500-2000
IHC 1:50-200
ICC/IF 1:50-200
FC 1:50
Positive Control
WB: HeLa cell, A549 cell
Validation Images & Assay Conditions
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Western blot analysis of PHD1/EGLN2 using anti-PHD1/EGLN2 antibody (M03015).
Electrophoresis was performed on a 10% SDS-PAGE gel at 80V (Stacking gel) / 120V (Resolving gel) for 2 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions.
Lane 1: human Hela whole cell lysates,
Lane 2: human A549 whole cell lysates,
Lane 3: human SIHA whole cell lysates,
Lane 4: human RT4 whole cell lysates,
Lane 5: rat testis tissue lysates,
Lane 6: rat C6 whole cell lysates,
Lane 7: mouse testis tissue lysates,
Lane 8: mouse Neuro-2a whole cell lysates.
After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-PHD1/EGLN2 antigen affinity purified monoclonal antibody (M03015) at 1:500 overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an ECL Plus Western Blotting Substrate (Catalog # AR1196-200) with Tanon 5200 system. A specific band was detected for PHD1/EGLN2 at approximately 48 kDa. The expected band size for PHD1/EGLN2 is at 44 kDa.
Specific Publications For Anti-PHD1/prolyl hydroxylase Rabbit Monoclonal Antibody (M03015)
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6 Customer Q&As for Anti-PHD1/prolyl hydroxylase Rabbit Monoclonal Antibody
Question
My question regards to test anti-PHD1/prolyl hydroxylase Rabbit Monoclonal antibody M03015 on mouse fetal brain for research purposes, then I may be interested in using anti-PHD1/prolyl hydroxylase Rabbit Monoclonal antibody M03015 for diagnostic purposes as well. Is the antibody suitable for diagnostic purposes?
Verified Customer
Verified customer
Asked: 2019-12-23
Answer
The products we sell, including anti-PHD1/prolyl hydroxylase Rabbit Monoclonal antibody M03015, are only intended for research use. They would not be suitable for use in diagnostic work. If you have the means to develop a product into diagnostic use, and are interested in collaborating with us and develop our product into an IVD product, please contact us for more discussions.
Boster Scientific Support
Answered: 2019-12-23
Question
Will M03015 anti-PHD1/prolyl hydroxylase Rabbit Monoclonal antibody work on parafin embedded sections? If so, which fixation method do you recommend we use (PFA, paraformaldehyde, other)?
Verified Customer
Verified customer
Asked: 2019-05-21
Answer
It shows on the product datasheet, M03015 anti-PHD1/prolyl hydroxylase Rabbit Monoclonal antibody as been validated on IF. It is best to use PFA for fixation because it has better tissue penetration ability. PFA needs to be prepared fresh before use. Long term stored PFA turns into formalin, as the PFA molecules congregate and become formalin.
Boster Scientific Support
Answered: 2019-05-21
Question
We are currently using anti-PHD1/prolyl hydroxylase Rabbit Monoclonal antibody M03015 for human tissue, and we are content with the IF results. The species of reactivity given in the datasheet says human, mouse, rat. Is it true that the antibody can work on primate tissues as well?
G. Krishna
Verified customer
Asked: 2018-11-19
Answer
The anti-PHD1/prolyl hydroxylase Rabbit Monoclonal antibody (M03015) has not been tested for cross reactivity specifically with primate tissues, but there is a good chance of cross reactivity. We have an innovator award program that if you test this antibody and show it works in primate you can get your next antibody for free. Please contact me if I can help you with anything.
Boster Scientific Support
Answered: 2018-11-19
Question
My question regarding product M03015, anti-PHD1/prolyl hydroxylase Rabbit Monoclonal antibody. I was wondering if it would be possible to conjugate this antibody with biotin. I would need it to be without BSA or sodium azide. I am planning on using a buffer exchange of sodium azide with PBS only. Would there be problems for me to conjugate the antibody and store it in -20 degrees in small aliquots?
S. Li
Verified customer
Asked: 2018-03-08
Answer
It is not recommended storing this antibody with PBS buffer only in -20 degrees. If you want to store it in -20 degrees it is best to add some cryoprotectant like glycerol. If you want carrier free M03015 anti-PHD1/prolyl hydroxylase Rabbit Monoclonal antibody, we can provide it to you in a special formula with trehalose and/or glycerol. These molecules will not interfere with conjugation chemistry and provide a good level of protection for the antibody from degradation. Please be sure to specify this in your purchase order.
Boster Scientific Support
Answered: 2018-03-08
Question
I see that the anti-PHD1/prolyl hydroxylase Rabbit Monoclonal antibody M03015 works with IF, what is the protocol used to produce the result images on the product page?
L. Wu
Verified customer
Asked: 2017-12-26
Answer
You can find protocols for IF on the "support/technical resources" section of our navigation menu. If you have any further questions, please send an email to support@bosterbio.com
Boster Scientific Support
Answered: 2017-12-26
Question
Is a blocking peptide available for product anti-PHD1/prolyl hydroxylase Rabbit Monoclonal antibody (M03015)?
Verified Customer
Verified customer
Asked: 2017-10-02
Answer
We do provide the blocking peptide for product anti-PHD1/prolyl hydroxylase Rabbit Monoclonal antibody (M03015). If you would like to place an order for it please contact support@bosterbio.com and make a special request.
Boster Scientific Support
Answered: 2017-10-02
