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Product Info Summary
| SKU: | M02182 |
|---|---|
| Size: | 100 μl/vial |
| Reactive Species: | Human, Mouse, Rat |
| Host: | Rabbit |
| Application: | IP, IF, ICC, WB |
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Product info
Product Name
Anti-PHD3 Rabbit Monoclonal Antibody
View all EGLN3/PHD3 Antibodies
SKU/Catalog Number
M02182
Size
100 μl/vial
Form
Liquid
Description
Boster Bio Anti-PHD3 Rabbit Monoclonal Antibody catalog # M02182. Tested in WB, ICC/IF, IP applications. This antibody reacts with Human, Mouse, Rat.
Storage & Handling
Store at -20°C for one year. For short term storage and frequent use, store at 4°C for up to one month. Avoid repeated freeze-thaw cycles.
Cite This Product
Anti-PHD3 Rabbit Monoclonal Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # M02182)
Host
Rabbit
Contents
Rabbit IgG in phosphate buffered saline, pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol, 0.4-0.5mg/ml BSA.
Clonality
Monoclonal
Clone Number
27E10
Isotype
IgG
Immunogen
A synthesized peptide derived from human PHD3
*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.
Reactive Species
M02182 is reactive to Egln3 in Human, Mouse, Rat
Applications
M02182 is guaranteed for IP, IF, ICC, WB Boster Guarantee
Observed Molecular Weight
27 kDa
Calculated molecular weight
27.242kDa
Background of EGLN3/PHD3
F-actin cross-linking protein which is thought to anchor actin to a variety of intracellular structures. This is a bundling protein. Probably involved in vesicular trafficking via its association with the CART complex. The CART complex is necessary for efficient transferrin receptor recycling but not for EGFR degradation.
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
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Assay dilution & Images
Reconsitution
Restore with deionized water (or equivalent) for reconstitution volume of 1.0 mL
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
WB 1:500-1:2000
ICC/IF 1:50-1:200
IP 1:50
Validation Images & Assay Conditions
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Western blot analysis of PHD3 expression in (1) A549 cell lysate; (2) NIH/3T3 cell lysate.
Protein Target Info & Infographic
Gene/Protein Information For Egln3 (Source: Uniprot.org, NCBI)
Gene Name
Egln3
Full Name
Egl nine homolog 3
Weight
27.242kDa
Alternative Names
EC 1.14.11; EC 1.14.11.-; EGL nine (C.elegans) homolog 3; egl nine homolog 3 (C. elegans); egl nine homolog 3; egl nine-like protein 3 isoform; EGLN3; FLJ21620; HIF prolyl hydroxylase 3; HIFPH3 MGC125998; HIFPH3; HIF-PH3; HIF-prolyl hydroxylase 3; HPH-1; HPH-3; Hypoxia-inducible factor prolyl hydroxylase 3; MGC125999; pdh3; PHD3; Prolyl hydroxylase domain-containing protein 3 Egln3|PHD-3, PHD3, SM-20|egl-9 family hypoxia-inducible factor 3|prolyl hydroxylase EGLN3|HIF-PH3|HIF-prolyl hydroxylase 3|HPH-3|egl nine homolog 3, mitochondrial|factor-responsive smooth muscle protein|hypoxia-inducible factor prolyl hydroxylase 3|prolyl hydroxylase domain-containing protein 3
*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".For more info on Egln3, check out the Egln3 Infographic
We have 30,000+ of these available, one for each gene! Check them out.
In this infographic, you will see the following information for Egln3: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].
Specific Publications For Anti-PHD3 Rabbit Monoclonal Antibody (M02182)
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No publications found for M02182
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Customer Reviews
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