|Validated Species:||Human, Rat|
Data & Images
|Product Name||Anti-SIRT3/Sirtuin 3 Picoband™ Antibody|
|Description||Rabbit IgG polyclonal antibody for NAD-dependent protein deacetylase sirtuin-3, mitochondrial(SIRT3) detection. Tested with WB in Human;Rat.|
|Cite This Product||Anti-SIRT3/Sirtuin 3 Picoband™ Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # PB9161)|
|Replacement Item||This antibody may replace the following items: sc-49744|sc-49743|sc-99143|sc-365175|sc-135796|sc-135795 from Santa Cruz Biotechnology.|
|Validated Species||Human, Rat|
*Our Boster Guarantee covers the use of this product in the above tested applications.
**For positive and negative control design, consult "Tissue specificity" under Protein Target Info.
|Recommended Detection Systems||Boster recommends Enhanced Chemiluminescent Kit with anti-Rabbit IgG (EK1002) for Western blot.
*Blocking peptide can be purchased at $50. Contact us for more information
**Boster also offers various secondary antibodies for Immunoflourescecne and IHC. Take advantage of the buy 1 primary antibody get 1 secondary antibody for free promotion for the entire year 2018!
|Immunogen||E.coli-derived human SIRT3 recombinant protein (Position: P66-K399). Human SIRT3 shares 86% amino acid (aa) sequence identity with mouse SIRT3.|
|Cross Reactivity||No cross reactivity with other proteins|
|Contents||Each vial contains 5mg BSA, 0.9mg NaCl, 0.2mg Na2HPO4, 0.05mg NaN3.|
|Concentration||Add 0.2ml of distilled water will yield a concentration of 500ug/ml.|
|Storage||At -20°C for one year. After reconstitution, at 4°C for one month. It can also be aliquotted and stored frozen at -20°C for a longer time.Avoid repeated freezing and thawing.|
|Purification||Immunogen affinity purified.|
Protein Target Info (Source: Uniprot.org)
You can check the tissue specificity below for information on selecting positive and negative control.
|Protein Name||NAD-dependent protein deacetylase sirtuin-3, mitochondrial|
|Molecular Weight||43573 MW|
|Protein Function||NAD-dependent protein deacetylase. Activates or deactivates mitochondrial target proteins by deacetylating key lysine residues. Known targets include ACSS1, IDH, GDH, SOD2, PDHA1, LCAD, SDHA and the ATP synthase subunit ATP5O. Contributes to the regulation of the cellular energy metabolism. Important for regulating tissue-specific ATP levels. .|
|Tissue Specificity||Widely expressed. .|
|Sequence Similarities||Belongs to the sirtuin family. Class I subfamily.|
|Subcellular Localization||Mitochondrion matrix .|
|Alternative Names||NAD-dependent protein deacetylase sirtuin-3, mitochondrial;hSIRT3;3.5.1.-;Regulatory protein SIR2 homolog 3;SIR2-like protein 3;SIRT3;SIR2L3;|
|Research Areas|||epigenetics and nuclear signaling|chromatin modifying enzymes|acetylation| cell biology|cell cycle|cell division|other cell division antibodies| epigenetics and nuclear signaling|hdacs|class iii / sir2 class| cancer|oncoproteins/suppressors|tumor suppressors| cardiovascular|heart|cardiac metabolism| metabolism|types of disease|obesity||
Background for NAD-dependent protein deacetylase sirtuin-3, mitochondrial
Dilution Ratios/Recommended Concentrations
At Boster we strive to provide the best Anti-SIRT3/Sirtuin 3 Picoband™ Antibody by testing all applications on non-spiked tissues and cell lines to ensure that the affinity of the antibody is enough to react to the endogenouse level of the target protein. Read more about our QC panel here.
|Recommended dilution ratios are listed below:|
Western blot, 0.1-0.5μg/ml, Human, Rat|
**Boster provides high sensitivity secondary antibody kits for Western blotting and IHC. For more info see Related Products below.
Anti-SIRT3/Sirtuin 3 Picoband™ Antibody Images
Click the images to enlarge.
All lanes: Anti SIRT3 (PB9161) at 0.5ug/ml
WB: Recombinant Human SIRT3 Protein 0.5ng
Predicted bind size: 39KD
Observed bind size: 39KD
All lanes: Anti SIRT3 (PB9161) at 0.5ug/ml
Lane 1: Rat Spleen Tissue Lysate at 50ug
Lane 2: Human Placenta Tissue Lysate at 50ug
Lane 3: HELA Whole Cell Lysate at 40ug
Lane 4: COLO320 Whole Cell Lysate at 40ug
Predicted bind size: 44KD
Observed bind size: 44KD
1. Post-translational modification:phosphorylation, methylation, glycosylation etc. These modifications prevent SDS molecules from binding to the target protein and thus make the band size appear larger than expected
2. Post-translational cleavage: this can cause smaller bands and or multiple bands
3. Alternative splicing: the same gene can have alternative splicing patterns generating different size proteins, all with reactivities to the antibody.
4. Amino Acid R chain charge: SDS binds to positive charges. The different size and charge of the Amino Acid side chains can affect the amount of SDS binding and thus affect the observed band size.
5. Multimers: Multimers are usually broken up in reducing conditions. However if the interactions between the multimers are strong, the band may appear higher.,