Anti-PHF8 Monoclonal Antibody

PHF8 antibody

Boster Bio Anti-PHF8 Monoclonal Antibody catalog # A03288. Tested in WB applications. This antibody reacts with Human, Mouse, Pig, Rat.

Product Info Summary

SKU: A03288
Size: 100μl
Reactive Species: Human, Mouse, Pig, Rat
Host: Mouse
Application: WB

Product Name

Anti-PHF8 Monoclonal Antibody

View all PHF8 Antibodies

SKU/Catalog Number

A03288

Size

100μl

Form

Liquid

Description

Boster Bio Anti-PHF8 Monoclonal Antibody catalog # A03288. Tested in WB applications. This antibody reacts with Human, Mouse, Pig, Rat.

Storage & Handling

Store at -20°C for one year. For short term storage and frequent use, store at 4°C for up to one month. Avoid repeated freeze-thaw cycles.

Cite This Product

Anti-PHF8 Monoclonal Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # A03288)

Host

Mouse

Contents

Purified mouse monoclonal in buffer containing 0.1M Tris-Glycine (pH 7.4, 150 mM NaCl) with 0.2% sodium azide, 50% glycerol.

Clonality

Monoclonal

Isotype

IgG

Immunogen

Purified recombinant human PHF8 (C-terminal) protein fragments expressed in E.coli.

*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.

Reactive Species

A03288 is reactive to PHF8 in Human, Mouse, Pig, Rat

Applications

A03288 is guaranteed for WB Boster Guarantee

Observed Molecular Weight

39 kDa

Calculated molecular weight

117.864kDa

Antibody Validation

Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.

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Assay Dilutions Recommendation

The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.

WB, 1:1000-1:2000

Validation Images & Assay Conditions

Gene/Protein Information For PHF8 (Source: Uniprot.org, NCBI)

Gene Name

PHF8

Full Name

Histone lysine demethylase PHF8

Weight

117.864kDa

Superfamily

JHDM1 histone demethylase family

Alternative Names

EC 1.14.11.27; histone lysine demethylase PHF8; jumonji C domain-containing histone demethylase 1F; KIAA1111ZNF422JHDM1F; MRXSSD; PHD finger protein 8DKFZp686E0868 PHF8 JHDM1F, KDM7B, MRXSSD, ZNF422 PHD finger protein 8 histone lysine demethylase PHF8|[histone H3]-dimethyl-L-lysine(36) demethylase PHF8|[histone H3]-dimethyl-L-lysine(9) demethylase PHF8|jumonji C domain-containing histone demethylase 1F

*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".

For more info on PHF8, check out the PHF8 Infographic

PHF8 infographic

We have 30,000+ of these available, one for each gene! Check them out.

In this infographic, you will see the following information for PHF8: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].

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5 Customer Q&As for Anti-PHF8 Monoclonal Antibody

Question

Is this A03288 anti-PHF8 Monoclonal antibody reactive to the isotypes of PHF8?

Verified Customer

Verified customer

Asked: 2019-10-10

Answer

The immunogen of A03288 anti-PHF8 Monoclonal antibody is Purified recombinant human PHF8 (C-terminal) protein fragments expressed in E.coli. Could you tell me which isotype you are interested in so I can help see if the immunogen is part of this isotype?

Boster Scientific Support

Answered: 2019-10-10

Question

I was wanting to use your anti-PHF8 Monoclonal antibody for WB for mouse cervix carcinoma erythroleukemia on frozen tissues, but I want to know if it has been tested for this particular application. Has this antibody been tested and is this antibody a good choice for mouse cervix carcinoma erythroleukemia identification?

C. Miller

Verified customer

Asked: 2019-07-17

Answer

You can see on the product datasheet, A03288 anti-PHF8 Monoclonal antibody has been validated for WB on human, mouse, pig, rat tissues. We have an innovator award program that if you test this antibody and show it works in mouse cervix carcinoma erythroleukemia in IHC-frozen, you can get your next antibody for free.

Boster Scientific Support

Answered: 2019-07-17

Question

I have a question about product A03288, anti-PHF8 Monoclonal antibody . I was wondering if it would be possible to conjugate this antibody with biotin. I would need it to be without BSA or sodium azide. I am planning on using a buffer exchange of sodium azide with PBS only. Would there be problems for me to conjugate the antibody and store it in -20 degrees in small aliquots?

D. Miller

Verified customer

Asked: 2019-05-22

Answer

We do not advise storing this antibody with PBS buffer only in -20 degrees. If you want to store it in -20 degrees it is best to add some cryoprotectant like glycerol. If you want carrier free A03288 anti-PHF8 Monoclonal antibody , we can provide it to you in a special formula with trehalose and/or glycerol. These molecules will not interfere with conjugation chemistry and provide a good level of protection for the antibody from degradation. Please be sure to specify this in your purchase order.

Boster Scientific Support

Answered: 2019-05-22

Question

Will anti-PHF8 Monoclonal antibody A03288 work for WB with cervix carcinoma erythroleukemia?

Verified Customer

Verified customer

Asked: 2019-01-25

Answer

According to the expression profile of cervix carcinoma erythroleukemia, PHF8 is highly expressed in cervix carcinoma erythroleukemia. So, it is likely that anti-PHF8 Monoclonal antibody A03288 will work for WB with cervix carcinoma erythroleukemia.

Boster Scientific Support

Answered: 2019-01-25

Question

Do you have a BSA free version of anti-PHF8 Monoclonal antibody A03288 available?

Verified Customer

Verified customer

Asked: 2018-05-03

Answer

We appreciate your recent telephone inquiry. I can confirm that some lots of this anti-PHF8 Monoclonal antibody A03288 are BSA free. For now, these lots are available and we can make a BSA free formula for you free of charge. It will take 3 extra days to prepare. If you require this antibody BSA free again in future, please do not hesitate to contact me and I will be pleased to check which lots we have in stock that are BSA free.

Boster Scientific Support

Answered: 2018-05-03

Order DetailsPrice
A03288

100uL

$399

Free Secondary Antibody

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A03288
Buy one primary antibody get one 0.5ml HRP or Biotin secondary antibody for free.
*Sample sizes are prepared on demand and will take extra lead time. (cannot be conjugated)
$399.00

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