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Product Info Summary
| SKU: | A04110-1 |
|---|---|
| Size: | 100 μg/vial |
| Reactive Species: | Human, Mouse, Rat |
| Host: | Rabbit |
| Application: | WB |
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Product info
Product Name
Anti-PHLPP2 Antibody Picoband®
SKU/Catalog Number
A04110-1
Size
100 μg/vial
Form
Lyophilized
Description
Boster Bio Anti-PHLPP2 Antibody Picoband® catalog # A04110-1. Tested in WB applications. This antibody reacts with Human, Mouse, Rat. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.
Storage & Handling
At -20°C for one year from date of receipt. After reconstitution, at 4°C for one month. It can also be aliquotted and stored frozen at -20°C for six months. Avoid repeated freezing and thawing.
Cite This Product
Anti-PHLPP2 Antibody Picoband® (Boster Biological Technology, Pleasanton CA, USA, Catalog # A04110-1)
Host
Rabbit
Contents
Each vial contains 4 mg Trehalose, 0.9 mg NaCl, 0.2 mg Na2HPO4.
Clonality
Polyclonal
Immunogen
A synthetic peptide corresponding to a sequence at the N-terminus of human PHLPP2. Human PHLPP2 shares 95.7% amino acid (aa) sequence identity with mouse PHLPP2.
Reactive Species
A04110-1 is reactive to PHLPP2 in Human, Mouse, Rat
Observed Molecular Weight
147 kDa
Calculated molecular weight
146.8 kDa
Background of PHLPP2
Predicted to enable metal ion binding activity and phosphoprotein phosphatase activity. Predicted to be involved in intracellular signal transduction. Located in several cellular components, including cilium; intercellular bridge; and mitotic spindle.
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
A04110-1 is guaranteed for WB Boster Guarantee
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
Western blot, 0.25-0.5 μg/ml, Human, Mouse, Rat
Validation Images & Assay Conditions
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Western blot analysis of PHLPP2 using anti-PHLPP2 antibody (A04110-1).
Electrophoresis was performed on a 8% SDS-PAGE gel at 80V (Stacking gel) / 120V (Resolving gel) for 2 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions.
Lane 1: human SIHA whole cell lysates,
Lane 2: human MCF-7 whole cell lysates,
Lane 3: human U251 whole cell lysates,
Lane 4: rat C6 whole cell lysates,
Lane 5: mouse small intestine tissue lysates,
Lane 6: mouse Neuro-2a whole cell lysates.
After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-PHLPP2 antigen affinity purified polyclonal antibody (A04110-1) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an ECL Plus Western Blotting Substrate (Catalog # AR1196-200) with Tanon 5200 system. A specific band was detected for PHLPP2 at approximately 147 kDa. The expected band size for PHLPP2 is at 147 kDa.
Specific Publications For Anti-PHLPP2 Antibody Picoband® (A04110-1)
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