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Product Info Summary
| SKU: | A10000 |
|---|---|
| Size: | 0.1 mg |
| Reactive Species: | Human, Mouse, Rat |
| Host: | Rabbit |
| Application: | ELISA, IF, IHC-P, WB |
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Product info
Product Name
Anti-Phosducin-like protein 3 PDCL3 Antibody
SKU/Catalog Number
A10000
Size
0.1 mg
Form
Liquid
Description
Boster Bio Anti-Phosducin-like protein 3 PDCL3 Antibody (Catalog # A10000). Tested in ELISA, WB, IHC-P, IF applications. This antibody reacts with Human, Mouse, Rat.
Storage & Handling
PDCL3 antibody can be stored at 4°C for three months and -20°C, stable for up to one year.
Cite This Product
Anti-Phosducin-like protein 3 PDCL3 Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # A10000)
Host
Rabbit
Contents
PDCL3 antibody is supplied in PBS containing 0.02% sodium azide.
Clonality
Polyclonal
Clone Number
Clone: SK7
Isotype
IgG
Immunogen
PDCL3 antibody was raised against a 14 amino acid peptide near the carboxy terminus of human PDCL3. The immunogen is located within the last 50 amino acids of PDCL3.
*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.
Cross-reactivity
PDCL3 antibody is human, mouse and rat reactive. PDCL3 antibody is predicted to not cross-react with other members of the PDCL protein family.
Reactive Species
A10000 is reactive to Pdcl3 in Human, Mouse, Rat
Applications
A10000 is guaranteed for ELISA, IF, IHC-P, WB Boster Guarantee
Observed Molecular Weight
68 kDa
Calculated molecular weight
27.581kDa
Background of Pdcl3
Phosducin-like proteins (PhLPs) are a conserved family of proteins with thioredoxin-like domains that were initially identified as modulators of G protein signaling (1,2). PDCL3 is highly homologous to PDCL and shares an N-terminal helix domain and a C-terminal thioredoxin-fold (Trx-fold) domain (3). Along with the related protein PDCL2, PDCL3 interacts with the chaperonin CCT and modulates CCT-mediated actin and tubulin folding (4). Modulation of PDCL3 levels by MAPK phosphorylation and RhoA-dependent changes also promote cytoskeletal remodeling (5).
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
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Assay dilution & Images
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
PDCL3 antibody can be used for detection of PDCL by Western blot at 1 - 2 μg/ml. Antibody can also be used for Immunohistochemistry at 5 μg/mL. For Immunoflorescence start at 20 μg/mL.
Antibody validated: Western Blot in human samples; Immunohistochemistry in mouse samples and Immunofluorescence in mouse samples. All other applications and species not yet tested. Optimal dilutions for each application should be determined by the researcher.
Validation Images & Assay Conditions
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Western blot analysis of PDCL3 in human brain tissue lysate with PDCL3 antibody at 1 μg/ml.
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Immunohistochemistry of PDCL3 in mouse lver tissue with PDCL3 antibody at 5 μg/mL.
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Immunofluorescence of PDCL3 in mouse liver tissue with PDCL3 antibody at 20 μg/mL.
Protein Target Info & Infographic
Gene/Protein Information For Pdcl3 (Source: Uniprot.org, NCBI)
Gene Name
Pdcl3
Full Name
Phosducin-like protein 3
Weight
27.581kDa
Superfamily
phosducin family
Alternative Names
HTPHLP; IAP-associated factor VIAF1; MGC3062; PHLP3; phosducin-like 3; phosducin-like protein 3; phPL3; VIAF; VIAF-1; VIAF1PHLP2A; Viral IAP-associated factor 1
*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".For more info on Pdcl3, check out the Pdcl3 Infographic
We have 30,000+ of these available, one for each gene! Check them out.
In this infographic, you will see the following information for Pdcl3: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].
Specific Publications For Anti-Phosducin-like protein 3 PDCL3 Antibody (A10000)
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No publications found for A10000
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